牡荆提取物对肉鸡坏死性肠炎的预防作用

doi:10.16431/j.cnki.1671-7236.2025.06.026

Abstract

Abstract: 【Objective】 This study was aimed to evaluate the preventive effects of Vitex negundo L.var.cannabifolia (Sieb.et Zucc.) Hand.-Mazz.extract (VNE) on the growth performance,serum immunoglobulin content,immune organ index,intestinal tissue structure,and inflammatory factors of broilers with necrotic enteritis (NE) induced by Clostridium perfringen (CP). 【Method】 150 male Lingnan Yellow-feather broilers with 7-day-old were randomly divided into control group (CON),infection group (INF),and low (L-VNE),medium (M-VNE),and high (H-VNE) VNE groups,with 3 replicates in each group and 10 broilers in each replicate.Broilers in CON group were fed with basal diet,and broilers in INF,L-VNE,M-VNE,and H-VNE groups were fed with high fishmeal diet.During 7-13 days of age,broilers in L-VNE,M-VNE,and H-VNE groups were orally administered with VNE at a dose of 4,8,and 12 g/(kg·d),respectively.Continuous gavage of 5×10⁹ CFU/d CP was administered to broilers in INF,L-VNE,M-VNE,and H-VNE groups during 10-13 days of age.Broilers were weighed at 14 and 21 days of age,and the average daily feed intake (ADFI),average daily gain (ADG),feed/gain (F/G) were calculated.After weighing,3 broilers were randomly selected for blood collection in each replicate,and the contents of IgA and IgG in serum were detected.After euthanized,the spleen,thymus,and bursa of Fabricius in broilers were separated and weighed,and the immune organ indexes were calculated.The pathological and morphological changes of duodenum,jejunum,and ileum in broilers were detected.Small intestine mucosa was scraped to analyze the expression of tumor necrosis factor-α (TNF-α),interleukin-8 (IL-8),IL-17A,and IL-1β.【Result】 ①At 14 days of age,compared with INF group,the ADFI and ADG of broilers in L-VNE and H-VNE groups were extremely significantly or significantly increased (P＜0.01 or P＜0.05),the content of serum IgG in H-VNE group was extremely significantly increased (P＜0.01).②At 21 days of age,the spleen index of broilers in M-VNE and H-VNE groups were extremely significantly higher than that in INF group (P＜0.01).③At 14 days of age,compared with INF group,the duodenum villus height (VH) of broilers in M-VNE and H-VNE groups was extremely significantly increased (P＜0.01),the VH of jejunum and ileum,and the VH to crypt depth (CD) ratio (VH/CD) of duodenum in L-VNE,M-VNE,and H-VNE groups were extremely significantly or significantly increased (P＜0.01 or P＜0.05),and the CD of duodenum was extremely significantly decreased (P＜0.01).The jejunum VH/CD of broilers in M-VNE and H-VNE groups significantly or extremely significantly increased (P＜0.05 or P＜0.01),and the ileum VH/CD in L-VNE and H-VNE groups was extremely significantly increased (P＜0.01).At 21 days of age,compared with INF group,the VH of duodenum,jejunum,and ileum of broilers in L-VNE,M-VNE,and H-VNE groups were extremely significantly or significantly increased (P＜0.01 or P＜0.05).④At 14 days of age,compared with CON group,the expression of intestinal IL-8 gene of broilers in L-VNE,M-VNE,and H-VNE groups were extremely significantly decreased (P＜0.01),and the expression of IL-1β gene in M-VNE group was significantly decreased (P＜0.05).【Conclusion】 Adding VNE to the feed of Lingnan Yellow-feather broilers infected with CP could improve the growth performance,increase the immunoglobulin content of serum,promote spleen development,alleviate intestinal lesions and inflammatory reactions.The results indicated that VNE could prevent necrotic enteritis induced by CP in broilers.Under the condition of this experiment,continuous gavage of 8 g/(kg·d) VNE to broilers for 7 days could achieve good prevention effects.

Key words: Vitex negundo L.var.cannabifolia (Sieb.et Zucc.)Hand.-Mazz.extract; Clostridium perfringens; broilers; necrotic enteritis; prevention

CLC Number:

S859.7

YUAN Minggui, LI Yahui, HE Junheng, TANG Xinggang, BA Xiaomin, ZHAO Yajun, TIAN Ya, LAI Xingru, XIANG Rong, YANG Xi. Prevention Effect of Vitex negundo L.var.cannabifolia (Sieb.et Zucc.) Hand.-Mazz.Extract on Necrotic Enteritis in Broilers[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2736-2749.

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Prevention Effect of Vitex negundo L.var.cannabifolia (Sieb.et Zucc.) Hand.-Mazz.Extract on Necrotic Enteritis in Broilers

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