Abstract: Oocyte cryopreservation by vitrification,as a technique for long-term preservation of oocytes at ultra-low temperatures,is of great significance in the preservation of excellent germplasm resources.However,the key difficulty of cryopreservation is the activation of apoptotic signals,which leads to an increase in the proportion of apoptotic cells and affects cell function,viability and developmental potential.Apoptosis refers to a programmed cell death process,often referred to as "cell suicide",and this mechanism plays a key role in living organisms,helping to maintain cell balance and tissue homeostasis.However,abnormal apoptosis can lead to adverse effects such as cell death.Therefore,effectively inhibiting or slowing down the apoptotic process becomes the key to improve the survival and developmental potential of frozen oocytes.The application of apoptosis inhibitors and antioxidants can reduce the apoptosis of vitrification oocytes,improve their survival rate and development ability,and thus improve the cell quality after thawing.The authors briefly describe the effects of vitrification cryopreservation technology on oocytes,and the endogenous and exogenous apoptosis mechanisms induced by freezing,and analyzed the application of related apoptosis inhibitors and antioxidants.In the future,the mechanism of action of new inhibitors and antioxidants in vitrification cryopreservation technology can be further explored.And explore the interaction between apoptosis and necrotic apoptosis and other cell death pathways,to provide a new idea and method for oocyte vitrification cryopreservation technology.

Key words: cryopreservation techniques; oocyte; apoptotic; apoptosis inhibitor; antioxidant