苦豆子和苦豆子生物碱对滩羊生长性能和瘤胃微生物区系的影响

doi:10.16431/j.cnki.1671-7236.2025.06.008

Abstract

Abstract: 【Objective】 This study aimed to investigate the effects of Sophora alopecuroides and alkaloids from Sophora alopecuroides on the growth performance and rumen microbial community of Tan sheep.【Method】 Sixty healthy Tan sheep in the fattening period with similar body weight (28.38 kg±1.49 kg) were randomly divided into five groups with 12 replicates each.The sheep in control group was fed a basal diet,and the sheep in trial groups 1，2 and 3 were fed the basal diet supplemented with 2%,3% and 4% Sophora alopecuroides respectively,the sheep in trial group 4 was fed the basal diet supplemented with 0.2% alkaloids from Sophora alopecuroides.The pre-feeding period was 7 days,and the feeding period was 60 days.The sheep were weighed during the trial,and their feed intake was recorded to assess growth performance.The rumen fluid samples were collected for the rumen microbial community dynamics in Tan sheep based on 16S rRNA high-throughput sequencing technology at the ending of the experiment.【Result】 ① Compared with the control group,the average daily feed intake of Tan sheep in trial group 3 was extremely significantly reduced (P＜0.01),and the feed gain ratio of the four trial groups were extremely significantly lower (P＜0.01).②There were no significant difference in the Ace,Chao1,Shannon,Simpson,and Sobs indexes of rumen microbiota among the groups (P＞0.05).③At the phylum level of the rumen microbiota in Tan sheep,Firmicutes and Bacteroidetes were the dominant bacterial phyla.Compared with the control group,the relative abundance of Firmicutes was extremely significantly decreased in trial groups 2 and 4 (P＜0.01),while the relative abundance of Firmicutes was extremely significantly increased in trial group 3 (P＜0.01).The relative abundance of Bacteroidetes and Fibrobacterota was both significantly increased (P＜0.05) in trial group 2.④At the genus level of the rumen microbiota in Tan sheep,the dominant rumen bacteria were Prevotella,Ruminococcus,Christensenellaceae R-7 group,Rikenellaceae RC9 gut group,and the norank_f_F082 group.Compared with the control group,the relative abundance of Prevotella in trial groups 1 and 2,the relative abundance of norank_f_F082 in trial group 4,and the relative abundance of Ruminococcus_gauvreauii_group in trial group 3 were all increased extremely significantly (P＜0.01).Meanwhile,the relative abundance of Olsenella in all four trial groups were decreased significantly (P＜0.05),and the relative abundance of unclassified Ruminococcaceae in trial group 3 were increased significantly (P＜0.05).【Conclusion】 Under the conditions of this experiment,the inclusion of 2%,3% Sophora alopecuroides and 0.2% alkaloids from Sophora alopecuroides in the diet effectively enhanced the growth performance of Tan sheep,regulated their rumen microbial community composition,and improved nutrient utilization.These findings provided a foundation for further exploration of the potential mechanisms of Sophora alopecuroides and alkaloids from Sophora alopecuroides as feed additives.

Key words: Sophora alopecuroides; alkaloids from Sophora alopecuroides; Tan sheep; growth performance; rumen microbial community

CLC Number:

S816.7

ZHOU Lusong, ZHAO Yuanyuan, YANG Yuwei, ZHAO Qingyu, MA Qing, TANG Chaohua, ZHANG Huiyan, ZHANG Junmin, QIN Yang, QIN Yuchang. Effects of Sophora alopecuroides and Alkaloids from Sophora alopecuroides on Growth Performance and Rumen Microbiota of Tan Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2540-2551.

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Effects of Sophora alopecuroides and Alkaloids from Sophora alopecuroides on Growth Performance and Rumen Microbiota of Tan Sheep

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