表没食子儿茶素-3-没食子酸酯缓解顺铂所致大鼠急性肾损伤效果评价

doi:10.16431/j.cnki.1671-7236.2023.12.039

Abstract

Abstract: 【Objective】 This study was aimed to evaluate the protective effects of different concentrations of epigallocatechin gallate (EGCG) on cisplatin-induced acute kidney injury in rats,and to select the best concentration of EGCG to alleviate cisplatin nephrotoxicity.【Method】 Thirty Wistar rats were randomly divided into 5 groups:Normal control group (CON),cisplatin group (CIS),low dose (20 mg/kg) EGCG＋cisplatin group (LCE),medium dose (40 mg/kg) EGCG＋cisplatin group (CE) and high dose (80 mg/kg) EGCG＋cisplatin group (HCE).Rats in CON group and CIS group were perfused with normal saline (40 mg/kg) for 28 days,and CIS group was intraperitoneally injected with CIS (7 mg/kg) on the 26th day.Rats in LCE,MCE and HCE groups were given corresponding dose of EGCG for 28 days,CIS was injected intraperitoneally on the 26th day,and serum and kidney tissues were taken on the 29th day.Firstly,the changes of physiological and biochemical indexes of rats were detected during drug administration,then the pathological changes of kidney tissue of rats in each group were detected,the protective effect of EGCG on acute kidney injury induced by CIS was preliminarily evaluated,and the best effective concentration of EGCG was selected.Then the protein expression changes of apoptosis (Bax, Bcl-2 and Caspase-3) and inflammation (IL-6 and IL-10) were detected by Western blotting to further confirm the protective effect of EGCG.【Result】 Compared with CON group,the diet,drinking water and body weight of rats in CIS group decreased significantly on the 28th day,while urea nitrogen (BUN) and creatinine (CRE) increased significantly (P＜0.05).The renal tubules were obviously dilated,with protein cast,and the renal injury was serious.Compared with CIS group,the diet,water consumption and weight of rats in MCE group were increased significantly (P＜0.05),and the contents of BUN and CRE in serum were decreased significantly (P＜0.05).The renal tubular dilatation in MCE group was significantly improved,but there was no significant change in LCE and HCE groups.Therefore,40 mg/kg EGCG was selected for the follow-up experiment.Western blotting result showed that compared with CON group,the expressions of Bax,IL-6 and Caspase-3 in CIS group were increased significantly (P＜0.05),while the expressions of Bcl-2 and IL-10 decreased significantly (P＜0.05).Compared with CIS group,the expressions of Bax,IL-6 and Caspase-3 in MCE group were decreased significantly (P＜0.05),while the expressions of Bcl-2 and IL-10 were increased significantly (P＜0.05).【Conclusion】 EGCG had a protective effect on CIS-induced acute kidney injury in rats (40 mg/kg was the best),which was related to the inhibition of renal cell apoptosis and inflammation.

Key words: epigallocatechin gallate (EGCG); cisplatin; rat; acute kidney injury

CLC Number:

S854.5^＋3

WANG Xingping, CAO Jiahui, GOU Dandan, SHAO Yiwen, CHI Shipeng, YANG Chunxue, XU Enshuang. Effect of Epigallocatechin Gallate on Alleviating Acute Kidney Injury Induced by Cisplatin in Rats[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5186-5193.

References

[1] 刘玉荣,郭会苹.顺铂:从意外合成到抗癌应用[J].化学教育(中英文),2023,44(1):2-7. LIU Y R,GUO H P.Cisplatin:From accidental synthesis to anti-cancer application[J].Chemical Education,2023,44(1):2-7.(in Chinese)
[2] QZKOK A,EDELSTIN C L.Pathophysiology of cisplatin-induced acute kidney injury[J].Biomed Research International,2014,2014:967826.
[3] ZHANG J,YE Z W,TEW K D,et al.Cisplatin chemotherapy and renal function[J].Advances in Cancer Research,2021,152:305-327.
[4] CHEN X,WEI W,LI Y,et al.Hesperetin relieves cisplatin-induced acute kidney injury by mitigating oxidative stress,inflammation and apoptosis[J].Chemico-Blological Interactions,2019,308:269-278.
[5] WANG S,TANG S,CHEN X,et al.Pulchinenoside B4 exerts the protective effects against cisplatin-induced nephrotoxicity through NF-κB and MAPK mediated apoptosis signaling pathways in mice[J].Chemico-Blological Interactions,2020,331:109233.
[6] DUPRE T V,DOLL M A,SHAH P P,et al.Suramin protects from cisplatin-induced acute kidney injury[J].American Journal of Physiology-Renal Physology,2016,310(3):F248-58.
[7] YANG Y,ENG L,WU S,et al.LIGHT deficiency aggravates cisplatin-induced acute kidney injury by upregulating mitochondrial apoptosis[J].International Immunopharmacology,2020,89(Pt A):106999.
[8] KELLUM J A,ROMAGNANI P,ASHUNTANTANG G,et al.Acute kidney injury[J].Nature Reviews Disease Primers,2021,7(1):52.
[9] 朱思睿,屈哲,杨艳伟,等.尿液新型生物标志物对顺铂诱导大鼠急性肾早期损伤的诊断效能研究[J].药物评价研究,2022,45(6):1046-1051. ZHU S R,QU Z,YANG Y W,et al.Diagnostic efficacy of new urine biomarkers on cisplatin-induced acute renal injury in rats[J].Drug Evaluation Research,2022,45(6):1046-1051.(in Chinese)
[10] NIELSEN L K,BRACKER K,PRICE L L.Administration of fenoldopam in critically ill small animal patients with acute kidney injury:28 dogs and 34 cats (2008-2012)[J]. Journal of Veterinary Emergency and Critical Care(San Antonio),2015,25(3):396-404.
[11] 覃鲁珊,赵海平,赵艳玲,等.大黄蒽醌与鞣质对大鼠肝脏的保护和损伤双向作用[J].中国中西医结合杂志,2014,34(6):698-703. QIN L S,ZHAO H P,ZHAO Y L,et al.Bidirectional effects of rhubarb anthraquinone and tannin on liver protection and damage in rats[J].Chinese Journal of Integrated Traditional and Western Medicine,2014,34(6):698-703.(in Chinese)
[12] 张媛媛.EGCG通过减轻氧化应激与抑制NALP3炎性体对阿霉素肾病有保护作用[D].重庆:中国人民解放军陆军军医大学,2014. ZHANG Y Y.EGCG has a protective effect against doxorubicin nephropathy by reducing oxidative stress and inhibiting NALP3 inflammasome bodies[D].Chongqing:Chinese PLA Army Military Medical University,2014.(in Chinese)
[13] ZHONG Y,CHIOU Y S,PAN M H,et al.Anti-inflammatory activity of lipophilic epigallocatechin gallate (EGCG) derivatives in LPS-stimulated murine macrophages[J].Food Chemistry,2012,134(2):742-748.
[14] AVADHANI K S,MANIKKATH J,TIWARI M,et al.Skin delivery of epigallocatechin-3-gallate (EGCG) and hyaluronic acid loaded nano-transfersomes for antioxidant and anti-aging effects in UV radiation induced skin damage[J].Drug Delivery,2017,24(1):61-74.
[15] 胡相卡,刘作栋,赵苗鑫,等.EGCG对糖尿病大鼠肾脏的保护作用及机制研究[J].石河子大学学报(自然科学版),2022,40(2):251-258. HU X K,LIU Z D,ZHAO M X,et al.Protective effect and mechanism of EGCG on kidney of diabetic rats[J].Journal of Shihezi University(Natural Science Edition),2022,40(2):251-258.(in Chinese)
[16] 左松,张炯.表没食子儿茶素没食子酸酯对大鼠肾脏缺血再灌注损伤的保护作用[J].临床肾脏病杂志,2017,17(4):240-244. ZUO S,ZHANG J.Protective effect of epigallocatechin gallate on renal ischemia-reperfusion injury in rats[J].Journal of Clinical Nephrology,2017,17(4):240-244.(in Chinese)
[17] MA X,YAN L,ZHU Q,et al.Puerarin attenuates cisplatin-induced rat nephrotoxicity:The involvement of TLR4/NF-κB signaling pathway[J]. Public Library of Science, 2017,12(2):e0171612.
[18] LIU K D,GOLDSTEIN S L,VIJAYAN A,et al.AKI!Now initiative:Recommendations for awareness,recognition,and management of AKI[J].Clinical Journal of the American Society of Nephrology,2020,15(12):1838-1847.
[19] TANASE D M,GOSAV E M,RADU S,et al.The predictive role of the biomarker kidney molecule-1(KIM-1) in acute kidney injury (AKI) cisplatin-induced nephrotoxicity[J].International Journal of Molecular Sciences,2019,20(20):5238.
[20] LUO D,XU J,CHEN X,et al.(-)-epigallocatechin-3-gallate (EGCG) attenuates salt-induced hypertension and renal injury in Dahl salt-sensitive rats[J].Scientific Reports,2020,10(1):4783.
[21] SOUSSI A,GARGOURI M,MAGNÉ C,et al.(-)-epigallocatechin gallate (EGCG) pharmacokinetics and molecular interactions towards amelioration of hyperglycemia,hyperlipidemia associated hepatorenal oxidative injury in alloxan induced diabetic mice[J].Chemico-Biological Interactions,2022,368:110230.
[22] ALOTAIBI B,EL-MASRY T A,ELEKHNAWY E,et al.Aqueous core epigallocatechin gallate PLGA nanocapsules:Characterization,antibacterial activity against uropathogens,and in vivo reno-protective effect in cisplatin induced nephrotoxicity[J].Drug Delivery,2022,29(1):1848-1862.
[23] MOLDOVEANU T,CZABOTAR P E.BAX,BAK,and BOK:A coming of age for the BCL-2 family effector proteins[J].Cold Spring Harbor Perspectives in Biology,2020,12(4):a036319.
[24] 符武岛,曾敏,陈娟,等.表没食子儿茶素没食子酸酯通过抑制心肌细胞凋亡缓解心肌缺血再灌注损伤的机制研究[J].中国药房,2019,30(16):2187-2192. FU W D,ZENG M,CHEN J,et al.Study on the mechanism of epigallocatechin gallate alleviating myocardial ischemia-reperfusion injury by inhibiting myocardial apoptosis[J].China Pharmacy,2019,30(16):2187-2192.(in Chinese)
[25] SHAFEEK F,EL-KASHEF D H,ABU-ELSAAD N,et al.Epigallocatechin-3-gallate in combination with corticosteroids mitigates heat stress-induced acute kidney injury through modulating heat shock protein 70 and Toll-like receptor 4-dependent pathways[J].Phytotherapy Research, 2023,37(8):3559-3571.
[26] YU S J,JIANG R,MAZZU Y Z,et al.Epigallocatechin-3-gallate prevents triptolide-induced hepatic injury by restoring the Th17/Treg balance in mice[J].American Journal of Chinese Medicine,2016,44(6):1221-1236.

Effect of Epigallocatechin Gallate on Alleviating Acute Kidney Injury Induced by Cisplatin in Rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SUN Zhuwen, LIU Zhengqun, ZHU Longbo, LI Ning, LIU Yanrong, LIANG Shiyue, ZHENG Zi, MU Shuqin, YAN Jun, SUN Chao. Process Optimization and Structure Analysis of Calcium Oxide and Sodium Carbonate Pretreatment of Reed Straw [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3562-3572.
[2]	WANG Shouyuan, YUN Hongmei, SHI Mingyue, QIN Yunmeng, LI Xiong, CHEN Junzhou, ZHOU Chenbo, CAO Guoqing. Effect of Porcine RPL36A Gene on PK15 Cell Proliferation [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3035-3044.
[3]	CUI Liang, WANG Keyao, ZHANG Xinping, WANG Huimin, HAN Yufeng, ZHANG Juan, DENG Ruiqiang, KANG Jie, DUAN Zhibian. Study on the Regulation of Compound Traditional Chinese Medicine on Inflammation-proliferation of Lung in Broilers with Ascites Syndrome [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3373-3383.
[4]	WANG Hui, FENG Baoliang, XIANG Guangming, HUANG Lei, LIU Zhiguo, LI Kui, MU Yulian. Construction of iPAMs with CD163 Monoallelic Expression and Characterization of Their Mediating PRRSV Infection [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2617-2628.
[5]	ZHANG Jiaxiang, HAN Diangang, SHI Yaling, MAO Xiaoyue, ZHAO Kaiwei, DU Xuan, XIN Jige. Construction of IPEC-J2 Cell Lines with PPARγ Gene Knockout Mediated by CRISPR/Cas9 Technology [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2670-2677.
[6]	LI Zhixing, QING Yuhong, LI Zhiyuan, CAO Shaoqi, GAO Xinmei, ZENG Weibin, WANG Jing, HU Guangdong. Effects of Leonurine on Cryopreservation of Sheep Semen [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2801-2810.
[7]	TANG Haibo, JIANG Jiajia, CHEN Fenglian, YANG Jinlan, BAI Anbin, LIU Jinfeng, QIN Shaomin, WU Jianmin. Isolation, Identification and Genomic Characterization Analysis of Parvovirus Isolate from Bamboo Rat [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2843-2853.
[8]	ZENG Chengrong, LIU Xin, BI Wenwen, MEI Shihui, HE Guangxia, ZHANG Junjie, WEN Ming, ZHOU Bijun, CHEN Jiangfeng, JIANG Haibo. Effect of Sodium Butyrate on the Growth and Virulence of Aeromonas hydrophila [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2987-2997.
[9]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2380-2387.
[10]	LI Can, LI Chuchu, ZENG Wei, ZHANG Ning, JI Chunxiao, CHEN Tao. Recombinant Expression and Function Study of RegⅢγ Protein in Porcine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2414-2426.
[11]	CAO Qi, JIN Qing, YUAN Yi, FENG Yixue, KONG Danni, CHEN Xianghong, WU Bin, SONG Wenbo, TANG Xibiao. Epidemiological Investigation of Common Respiratory Bacterial Pathogens in Large-scale Pig Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2439-2449.
[12]	LIU Peng, LI Jingjing, QIAO Yanliang, ZHU Lianqin, ZHU Fenghua. Extraction and Structure Characterization of Scutellaria baicalensis Polysaccharide [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2518-2530.
[13]	NIU Yeyu, SHEN Qi, BAI Yong, SUN Panpan, SUN Na, YIN Wei, FAN Kuohai, LI Hongquan, SUN Yaogui. Anti-inflammatory and Antioxidative Effects of Extracts of Physalis Calyx seu Fructus Based on Ulcerative Colitis Model [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2531-2539.
[14]	PIAN Huifang, DU Xubin, LI Yan, ZHANG Yuchen, LIU Fei, YU Debing. Sequencing Analysis of the Full-length Transcriptome of Granulosa Cells of Small Yellow Follicle in Hy-Line Variety Brown [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1754-1763.
[15]	ZHANG Xiaomeng, JIAO Anhui, WANG Yuqi, FAN Nanzhu, JIN Qingguo. Effects of Rosiglitazone Supplementation on Redox Homeostasis of Oocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1818-1827.