乏情和发情初产母猪下丘脑-垂体轴中miRNA表达谱比较分析

doi:10.16431/j.cnki.1671-7236.2023.11.018

Abstract

Abstract: 【Objective】 The purpose of this study was to reveal the genetic mechanism of microRNA (miRNA) on estrous activity in primiparous sows from the neuroendocrine system.【Method】 RNA-Seq technology was utilized to sequence small RNA from the hypothalamic-pituitary axis of anestrous and estrous primiparous sows in this study.The miRNA expression profiles in the hypothalamus and pituitary of sows were obtained and the differentially expressed miRNA were screened for functional analysis such as GO and KEGG.【Result】 A total of 1 096 miRNAs were identified from four libraries, of which 364 were known miRNAs and 732 were novel miRNAs.Compared with estrous primiparous sows, 16 differentially expressed miRNAs were identified in the hypothalamus of anestrous sows, including 6 up-regulated and 10 down-regulated miRNAs.9 differentially expressed miRNAs were identified in the pituitary of anestrous sows, including 2 up-regulated and 7 down-regulated miRNAs.A total of 5 212 target genes were predicted for 16 differentially expressed miRNAs in the hypothalamus, and 6 897 target genes were predicted for 9 differentially expressed miRNAs in the pituitary gland.Functional analysis of differentially expressed miRNA target genes in hypothalamus showed some GO entries and KEGG signaling pathways were significantly enriched, such as cell projection assembly, plasma membrane bounded cell projection assembly, negative regulation of neuron projection development, protein serine/threonine kinase activity, Ras signaling pathway, neurotrophin signaling pathway, ErbB signaling pathway, and mTOR signaling pathway, etc.These miRNA target genes in pituitary were also significantly enriched in some GO entries and KEGG signaling pathways, such as regulation of mRNA processing, Ras GTPase binding, ubiquitin-like protein ligase activity, epidermal growth factor receptor binding, protein kinase, sphingolipids signaling pathway, apoptosis, ErbB signaling pathway, and NF-κB signaling pathway, etc.Chr3_8404_mature, ssc-miR-9 and ssc-miR-34c were screened from miRNA regulatory network with target genes that were involved in regulation of reproduction, and these 3 miRNAs might play an important role in regulating estrous activity in the hypothalamic-pituitary axis of primiparous sows.8 differentially expressed miRNAs (4 in hypothalamus and 4 in pituitary) were selected for validation by Real-time quantitative PCR, and their expression trends were generally consistent with the sequencing results.【Conclusion】 The miRNA expression profiles of hypothalamic-pituitary axis of anestrous and estrous primiparous sows were successfully constructed and the functions of differentially expressed miRNA were deeply explored in this study.3 miRNAs that play an important role in the estrous activity of primiparous sows were screened.A theoretical basis for the analysis of the genetic mechanism of estrous activity in primiparous sows had been provided.

Key words: miRNA; anestrus; estrus; primiparous sows; hypothalamic-pituitary axis

CLC Number:

S828

REN Qiaoling, ZHANG Jiaqing, WANG Jing, CHEN Junfeng, MA Qiang, GAO Binwen, LIU Fujiu, XING Baosong. Comparison and Analysis of miRNA Expression Profile in the Hypothalamic-pituitary Axis of Anestrous and Estrous Primiparous Sows[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4491-4503.

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Comparison and Analysis of miRNA Expression Profile in the Hypothalamic-pituitary Axis of Anestrous and Estrous Primiparous Sows

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