亚急性瘤胃酸中毒湖羊瘤胃液中菌群和B族维生素丰度变化的关联分析

doi:10.16431/j.cnki.1671-7236.2023.10.018

Abstract

Abstract: 【Objective】 The purpose of this study was to investigate the effects of subacute rumen acidosis(SARA)on the microbial flora structure and B vitamins expression abundance in rumen fluid of Hu sheep,and to analyze the correlation between them.【Method】 Twelve healthy Hu sheep were randomly divided into 2 groups,with 6 sheep in each group.The ratio of feed concentrate to forage in control group was 3∶7,and that in SARA group was 7∶3 to establish SARA model.The structural changes of bacteria were analyzed by 16S rRNA sequencing technology,the abundance of B vitamins expression in rumen fluid was analyzed by LC-MS method,and the correlation between them was analyzed by Pearson method.【Result】 The rumen microbial diversity index (Shannon and Chao1) of Hu sheep in control group was significantly higher than that in SARA group,and the microbial diversity was significantly higher than that in SARA group (P＜0.05).At the level of the phylum bacterialis,the abundance of Actinobacteria,Candidatus_Saccharibacteria and Tenericutes in control group were significantly higher than that in SARA group (P＜0.05).At the genus level,Bifidobacterium,Sharpea,Saccharibacteria,unclassified_Ruminococcaceae in control group were significantly higher than that in SARA group (P＜0.05),but unclassified_Desulfovibrionaceae,unclassified_Clostridiales,Alloprevotella were significantly lower than that in SARA group (P＜0.05).Six different metabolites,VB₆ (pyridoxal,pyridoxine,pyridoxine),VB₃,VB₉ and VB₁₂,were screened out from control group and SARA group,and their expression abundance in SARA group was significantly higher than that in control group (P＜0.05).Pearson correlation results showed that six different B vitamins species were significantly associated with the vast majority of rumen microorganisms (R＞0 or R＜0).【Conclusion】 SARA changed the phylum and genus level structure of bacteria in rumen fluid,resulting in decreased bacterial diversity and increased the abundance of VB₆,VB₃,VB₉ and VB₁₂ expression,which was significantly correlated with most of the bacteria in rumen fluid.

Key words: subacute rumen acidosis(SARA); microorganisms; B vitamins; abundance

CLC Number:

S816

WU Jianxin, YAN Zhenxing, DUAN Hongwei, LYU Jianshu, ZENG Jianlin, MA Xiaojun, HU Junjie. Correlation Analysis of Microflora and B Vitamins Abundance in Rumen Fluid of Hu Sheep in Subacute Rumen Acidosis[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4045-4057.

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Correlation Analysis of Microflora and B Vitamins Abundance in Rumen Fluid of Hu Sheep in Subacute Rumen Acidosis

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