高渗对多重耐药大肠杆菌外排泵和外膜孔道蛋白基因表达及其生长的影响

doi:10.16431/j.cnki.1671-7236.2021.09.040

Abstract

Abstract: In order to explore the effects of hypertonic stress on gene expression and growth of efflux pump and outer membrane protein of multidrug-resistant bacteria, growth curve and relative adaptability were used to compare the effects of different salt stress conditions on resistant Escherichia coli QL15 and sensitive Escherichia coli ATCC 25922. RT-PCR was used to investigate the difference of gene expression of efflux pump and outer membrane protein under salt stress. The results showed that Escherichia coli QL15 was a high-level multidrug-resistant bacterium, and the most serious resistance to spectinomycin and enrofloxacin was 32 times of the standard MIC. When cultured independently, Escherichia coli QL15 was more sensitive to salt stress than Escherichia coli ATCC 25922, and under 6.0% NaCl stress, Escherichia coli QL15 grew slowly within 10 h, but its concentration was higher at 24 h. When cultured in mixture, Escherichia coli QL15 had higher adaptability to 6.0% NaCl stress, and had more competitive advantage in vitro culture. Escherichia coli QL15 carried 15 drug resistance related genes in 5 categories, including 8 efflux pump genes and 3 outer membrane protein genes. The expression levels of 5 genes in Escherichia coli ATCC 25922 at 6.0% NaCl concentration were about 50% lower than that in 3.5% NaCl concentration (P<0.05). However, the expression of Escherichia coli QL15 were significantly up-regulated at 6.0% NaCl concentration except acrB and ompF genes (P<0.05). It was speculated that the higher adaptability of Escherichia coli QL15 to salt stress might be related to the expression of membrane associated proteins.

Key words: efflux pump; outer membrane protein; Escherichia coli; relative adaptability; salt stress

CLC Number:

S859.7

SUN Haifeng, TAN Aijuan, LYU Shiming, LUO Yujia, JI Qiqi, AI Renli, LU Xingxing, FENG Dan. Effect of Hypertonicity on Gene Expression of Efflux Pump and Outer Membrane Protein in Multidrug Resistant Escherichia coli and Its Growth[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3473-3482.

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Effect of Hypertonicity on Gene Expression of Efflux Pump and Outer Membrane Protein in Multidrug Resistant Escherichia coli and Its Growth

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