基于转录组学技术分析绿原酸对鸡源大肠杆菌抑菌及耐药消除的作用机制

doi:10.16431/j.cnki.1671-7236.2020.12.040

Abstract

Abstract: In order to explore the mechanism of chlorogenic acid on the inhibition and elimination of drug resistance of antibiotic resistant Escherichia coli from chicken,the third generation of avian Escherichia coli drug-resistant reverse strains,cultivated in the LB broth whose chlorogenic acid concentration was 1.25 mg/mL (1/2 MIC),was separated through photolithography,the minimal inhibitory concentration (MIC) of levofloxacin was determined through microplate method,and the molecular mechanism of chlorogenic acid eliminating drug resistance of Escherichia coli was further analyzed through transcriptome sequencing method.The results showed that the MIC of drug-resistant reverse strains to levofloxacin decreased from 16 μg/mL to 4-8 μg/mL,indicating that to a certain extent,chlorogenic acid of 1.25 mg/mL could eliminate the resistance of Escherichia coli to levofloxacin.To further analyze the molecular mechanism of chlorogenic acid on the elimination of drug resistance of Escherichia coli from chicken,transcriptome sequencing was used to compare the gene expression of this kind of Escherichia coli before and after the elimination of drug resistance.And it was found that the expression levels of 12 genes were significantly down-regulated after chlorogenic acid treatment,which the results of fluorescence quantitative PCR were basically consistent with.After GO functional enrichment analysis and KEGG metabolic pathway enrichment analysis,it was found that the differentially expressed genes were functionally concentrated in membrane composition and DNA recombination,and in metabolic pathway,the differentially expressed genes were related to metabolism.bhsA and cysP in the differentially expressed genes were Coli-HB chromatin transcribed RNA (GenBank No.:CP020933).oqxA,oqxR,emaA,pinR,xerD,folA,repA,repC,adhP,IS26 were Coli-HB plasmid 1 transcribed RNA (GenBank No.:CP020934).It could be seen from the distribution of differentially expressed genes that chlorogenic acid could weaken the resistance of bacteria,inhibit the activity of drug-resistant genes,and inhibit the recombination of bacterial DNA,etc.To some extent,it could eliminate the drug-resistance of Escherichia coli to levofloxacin and play a role in inhibiting the bacteria and eliminating the drug-resistance.

Key words: Escherichia coli; chlorogenic acid; drug resistance elimination; transcriptome

CLC Number:

S853.74

YAO Shanshan, ZHANG Shilei, LIANG Cunjun, ZHANG Tie, WANG Chunguang. Analysis of Bacteriostatic and Drug Resistance Elimination Mechanisms of Chicken-derived Escherichia coli by Chlorogenic Acid Based on Transcriptome Technology[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4156-4165.

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Analysis of Bacteriostatic and Drug Resistance Elimination Mechanisms of Chicken-derived Escherichia coli by Chlorogenic Acid Based on Transcriptome Technology

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