环境雌激素灭多威对牛卵母细胞体外培养质量的影响

doi:10.16431/j.cnki.1671-7236.2020.09.022

Abstract

Abstract: The aim of this study was to evaluate the effects of methotyl (MET) on the quality of in vitro cultured bovine oocytes.The quality of bovine oocytes was evaluated using the first polar body excretion rate,reactive oxygen species (ROS),glutathione (GSH) level,mitochondrial membrane potential (ΔΨm) and the expression of apoptosis-related gene.The results demonstrated that different concentration of MET had no effects on cumulus cells expansion,but the first polar body excretion rate was significantly lower than that of control group after administration of 200 μmol/L MET (P<0.05).The data also showed that 200 μmol/L MET could significantly increase ROS and decrease GSH and mitochondrial membrane potential (P<0.05).In addition,the mRNA transcription levels of Caspase-3 and Bax,which were pro-apoptotic genes,were significantly increased and the anti-apoptotic gene Bcl-xl was significantly reduced after MET administration (P<0.05).The results indicated that MET could increase oxidative stress and apoptosis which led to the reduction of bovine oocytes quality.

Key words: bovine; methomyl; oxidative stress; mitochondria; oocyte

CLC Number:

S823.8⁺1

HE Daohong, HAN Guobo, SUN Jingyu, CUI Yuxin, YIN Ying, ZHANG Xiaomeng, E Zhiqiang, GAO Qingshan. Effect of Environmental Estrogen Methomyl on the Quality of in vitro Culture Bovine Oocytes[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2884-2891.

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Effect of Environmental Estrogen Methomyl on the Quality of in vitro Culture Bovine Oocytes

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