鼠伤寒沙门菌LH430株asd基因缺失株平衡致死系统的构建及生物学特性分析

doi:10.16431/j.cnki.1671-7236.2019.11.024

Abstract

Abstract: This study was aimed to construct a balanced lethal system based on attenuated Salmonella Typhimurium.Cat gene was recombined into the position of asd gene in Salmonella Typhimurium LH430 strain,then the thermo sensitive plasmid pCP20 was transferred into the strain to eliminate the Cat gene fragment,and the mutant strain Δasd LH430 was identified by PCR.Then the balanced lethal system of Salmonella Typhimurium Δasd LH430(pYA3493) was successfully constructed with the plasmid pYA3493 transferred into Δasd LH430.The mutant strain lost the ability to survive in the medium without DAP,had the same ability of using carbon sources was consistent with parent strains,and inherited the biochemical characteristics from parent strains based on nine biochemical experiments,Δasd LH430(pYA-gfp) carrying green fluorescent gene was successfully constructed,and the green fluorescent protein could be also inherited the deletion fragment of 423 bp.Then the Δasd LH430(pYA-gfp) carrying green fluorescent gene was successfully constructed,and the green fluorescent protein could be effectively expressed by Δasd LH430(pYA-gfp).In conclusion,the balanced lethal system Δasd LH430(pYA3493) was successfully constructed in this study,and could effectively express the carrying genes,the system could be used as a living vaccine vector to carry exogenous genes.

Key words: Salmonella Typhimurium; balanced lethal system; living vaccine vector; biological characteristics

CLC Number:

R378.2⁺3

ZHANG Mingliang, SUN Changjiang, GU Jingmin, CUI Ziyin, HAN Wenyu. Construction and Biological Characteristics Analysis of asd Gene Mutant Balanced Lethal Host-vector System of Salmonella Typhimurium LH430 Strain[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3341-3349.

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Construction and Biological Characteristics Analysis of asd Gene Mutant Balanced Lethal Host-vector System of Salmonella Typhimurium LH430 Strain

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