伪狂犬病病毒FS-2015株gE和gB基因序列分析

doi:10.16431/j.cnki.1671-7236.2018.12.026

Abstract

Abstract:

To study the genetic mutation and evolution of gB and gE genes of porcine pesudorabies virus (PRV),the FS-2015 wild strain PRV was purified in the tissue disease by plaque assay method (three times),the whole gene fragments of gB and gE genes were amplified,and PCR products were sequenced and analyzed in this study.The results showed that the homology of nucleotide sequence of gB and gE genes of PRV FS-2015 strain with other PRV strains from GeneBank were 97.0% to 100.0% and 97.5% to 99.7%,the homology of amino acid sequence were 96.4% to 100.0% and 95.3% to 99.7%,respectively.The amino acid variation site analysis results showed that gB and gE genes of FS-2015 strain had site mutations and deletions.Phylogenetic tree analysis results showed that the PRV FS-2015 stain with GY,ZJ01,HB1201,HN1201,JS2012,BJ-YT and BP stains had closer relationship,while that with Kaplan,Becker,NIA3,Kolchis,Bartha and Yangsan strains had a distant relationship.The results of PRV FS-2015 strains and classical strains at home and abroad,and the currently domestic variant strains suggested that PRV FS-2015 strain belonged to the variant popular in recent years,which could provide data for the molecular epidemiological investigation,the prevention and control of pseudorabies and the selection of vaccine strains in Guangdong province.

Key words: pseudorabies virus (PRV); FS-2015 strain; gB gene; gE gene; sequence analysis

CLC Number:

S852.65⁺9.1

PANG Xuanfei, LIAN Sinan, WU Jianmin, WAN Zengpei, WANG Zhengfan, LI Zhongsheng, BAI Aiquan. Sequence Analysis of gE and gB Genes of Pseudorabies Virus FS-2015 Strain[J]. , 2018, 45(12): 3524-3534.

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Sequence Analysis of gE and gB Genes of Pseudorabies Virus FS-2015 Strain

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