11种牛分枝杆菌抗原在牛结核病诊断中的初步评价

doi:10.16431/j.cnki.1671-7236.2018.08.030

Abstract

Abstract:

To screen and evaluate antigens used for diagnosis of bovine tuberculosis (bTB),eleven Mycobacterium bovis (M.bovis) antigens of CFP-10,ESAT-6,TB10.4,TB27.4,MPT51,MPT63,MPT64,MPB70,MPB83,Rv3872 and Ag85B were used as coated antigen to establish indirect ELISA,the detection rates of ELISA based on each antigen were calculated.The potential of these recombinant proteins used as stimuli in skin test were assessed by skin test in guinea pigs and cattle.Besides,to evaluate the potential of these proteins used as stimuli in IFN-γ release assay,the heparinized blood from bTB positive and negative cattle were stimulated by recombinant proteins for 24 h,and IFN-γ in plasma were detected.The results indicated recombinant proteins showed virous reactivities to bTB positive serum,MPB70-based ELISA showed highest detection rate (59.7%),and followed by Ag85B,ESAT-6 and MPB83 (>45%).MPT51-based ELISA showed the lowest detection rate,which was only 2.2%.The results of skin tests on guinea pigs and cattle showed single recombinant protein as stimulus in skin test couldn't induce satisfactory delayed type hypersensitivity (DTH),while TB10.4,TB27.4,MPT64,MPT63 or Rv3872 as supplementary antigen for CFP-10 or ESAT-6,could induce high DTH in bTB positive cattle,and showed no difference with PPD-B (P>0.05).Whole blood from bTB positive cattle could release a certain level of IFN-γ induced by the recombinant protein CFP-10,ESAT-6,TB10.4 and MPT51.The IFN-γ induced by CFP-10,CFP-10-ESAT-6 and MPT51 were significantly higher in bTB positive cattle than negative cattle (P<0.05).Therefore,these eleven M.bovis antigens were not good for singly used in serological diagnosis,skin test,or IFN-γ release assay for bTB.However,TB10.4,TB27.4,MPT64,MPT63,Rv3872 or MPT51 as supplementary antigen for CFP-10 or ESAT-6 could improve the sensitivity,and be used as specific stimuli in skin test and IFN-γ release assay for the diagnosis of bTB.

Key words: Mycobacterium bovis; bovine tuberculosis; diagnosis; antibodies; skin test; IFN-γ release assay

CLC Number:

S852.61⁺8

GAO Xintao, JIA Hong, HOU Shaohua, YANG Hongjun, GUO Xiaoyu, YUAN Weifeng, JIANG Yitong, ZHU Hongfei, XIN Ting, DING Jiabo. Preliminary Evaluation of Eleven Mycobacterium bovis Antigens in the Diagnostic Methods of Bovine Tuberculosis[J]. , 2018, 45(8): 2282-2292.

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Preliminary Evaluation of Eleven Mycobacterium bovis Antigens in the Diagnostic Methods of Bovine Tuberculosis

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