6个绵羊群体BMP15、GDF9基因多态性分析

doi:10.16431/j.cnki.1671-7236.2018.08.024

Abstract

Abstract:

This study was aimed to detect the gene polymorphism of bone morphogenetic protein 15(BMP15) and growth differentiation factor 9 (GDF9) genes in Xinji Fine-wool sheep,Northeast Fine-wool sheep,Chinese Merino(Mulley type) sheep,Dorper×Han crossbred sheep (F1),Dorper×Han crossbred sheep (F3) and White Dorper sheep,which would provide a theoretical basis for marker-assisted selection and breeding for prolificacy in sheep.A total of 378 individuals from six sheep breeds were used to study the polymorphism of BMP15 and GDF9 genes by PCR-SSCP.Secondary structure and tertiary structure of proteins were predicted using DNAStar and I-TASSER.Gene frequency,genotype frequency,Hardy-Weinberg equilibrium,heterozygosity (He),homozygous (Ho),number of effective alleles (Ne) and polymorphic information content (PIC) were calculated.The correlation was analyzed between BMP15 gene polymorphism and litter size in Xinji Fine-wool sheep by GraphPad Prism 6 software.The results showed that the polymorphism was found in P1 primer amplification fragment of BMP15 gene,there were three genotypes of AA,AC and CC in six breeds.This mutation was a 58-60 bp (CTT) deletion in exon 1 of BMP15 gene.According to χ² test,Xinji Fine-wool sheep,Dorper×Han crossbred sheep (F3) and White Dorper sheep were not in Hardy-Weinberg equilibrium.There was no significant difference in the average litter size of different genotypes in Xinji Fine-wool sheep (P>0.05).The polymorphism was found in P2 primer amplification fragment of GDF9 gene.There were three genotypes of DD,DE and EE in six breeds,and there were only one genotype in Xinji Fine-wool sheep.This mutation was a T→C conversion at 477 bp on GDF9 gene exon 2 which didn't lead to amino acid change.According to χ² test,five breeds (except for Xinji Fine-wool sheep) were in Hardy-Weinberg equilibrium.The results indicated that the GDF9 gene T477C mutation couldn't be used as a genetic marker for multiple fetal traits in Xinji Fine-wool sheep.

Key words: sheep; BMP15 gene; GDF9 gene; polymorphism

CLC Number:

S813.3

WU Cuiling, ZONG Xinglong, ZHAO Zhuo, ZHAO Yunhui, ZHAI Bo, ZHANG Mingxin, WANG Chunxin. Genetic Polymorphism Analysis of BMP15 and GDF9 Genes in Six Sheep Breeds[J]. , 2018, 45(8): 2236-2246.

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Genetic Polymorphism Analysis of BMP15 and GDF9 Genes in Six Sheep Breeds

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