牦牛和犏牛睾丸CPT1a和ETHE1基因mRNA水平比较研究

doi:10.16431/j.cnki.1671-7236.2018.07.020

Abstract

Abstract:

This study was aimed to explore characteristics of fatty acid oxidation in the testes of sterile yak hybrids (cattle-yaks) and its association with male sterility,and elucidate the molecular mechanism of male sterility of cattle-yaks.A Real-time quantative PCR method was established to assay the mRNA levels of liver type-carnitine palmitoyl transferase 1a (CPT1a) and sulfide dioxygenase 1 (ETHE1) genes in yak and cattle-yak testes using GAPDH and 18S rRNA as reference genes.This Real-time quantative PCR showed high amplification specificity,with amplification rate ranging from 98.5% to 107.8% and qualified standard curves.The testes and epididymides of adult yaks (n=9) and sterile male cattle-yaks (n=7) were collected and detected with conventional histological sections and HE staining.Sperms were found in the testes and epididymides of yaks but not cattle-yaks.Total RNA were extracted from the testes of yaks and cattle-yaks for the analysis of CPT1a and ETHE1 genes mRNA.The results showed that the CPT1a and ETHE1 genes mRNA level of testicular in cattle-yaks were significantly and extremely significantly higher than those of yaks (P<0.05;P<0.01).The increased CPT1a gene mRNA level indicated the higher β-oxidation capacity in cattle-yak testes;While the elevated ETHE1 gene mRNA level could facilitate the electron transfer and fatty acid oxidation in mitochondria,or influence other energy metabolism pathways.The results preliminarily demonstrated that the testes of sterile cattle-yaks had increased oxidation of fatty acid for energy supply compared to yak testes,and might had more dependent on fatty acids as fuel.

Key words: yak; hybrid male sterility; carnitine palmitoyl transferase 1a (CPT1a); sulfide dioxygenase 1(ETHE1)

CLC Number:

S823.8⁺5

LI Fangwei, HUANG Lin, JIN Suyu, LEI Jiewen, FU Wei, ZHENG Yucai. Study on Comparison of CPT1a and ETHE1 Genes mRNA Levels in the Testes of Yaks and Cattle-yaks[J]. , 2018, 45(7): 1891-1897.

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Study on Comparison of CPT1a and ETHE1 Genes mRNA Levels in the Testes of Yaks and Cattle-yaks

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