6个绵羊群体骨形态蛋白受体1B基因多态性分析

doi:10.16431/j.cnki.1671-7236.2018.04.017

Abstract

Abstract:

The study was aimed to analyze the gene polymorphism of bone morphogenetic protein receptor-1B (BMPR-1B) gene in six sheep population,which would provide a theoretical basis for marker-assisted selection and breeding for prolificacy in sheep.The blood samples of a total of 381 individuals from six sheep breeds (Xinji Fine wool sheep,Northeast Fine wool sheep,Chinese Merino (Mulley type) sheep,Dorper×Han crossbred sheep (F1),Dorper×Han crossbred sheep (F3) and White Dorper sheep) were used to study the polymorphism of BMPR-1B gene by PCR-RFLP.Secondary structures of proteins were predicted using DNAStar,gene frequency,genotype frequency,Hardy-Weinberg equilibrium and genetic diversity parameters (heterozygosity (He),homozygous (Ho),effective allele (Ne),polymorphic information content (PIC)) were calculated by Excel 2003 software.The results showed that there were no polymorphism in Xinji Fine wool sheep,Northeast Fine wool sheep,Chinese Merino (Mulley type) sheep and White Dorper sheep.The polymorphism in Dorper×Han crossbred sheep (F1) and Dorper×Han crossbred sheep (F3) existed three kind of genotypes (++,B+ and BB).According to χ² text,Dorper×Han crossbred sheep (F1) were not in Hardy-weinberg equilibrium,and Dorper×Han crossbred sheep (F3) were in Hardy-weinberg equilibrium.The PIC were 0.311 and 0.264 in the Dorper×Han crossbred sheep (F1) and Dorper×Han crossbred sheep (F3),which both meaned moderate diversity.The BMPR-1B gene might be a major gene which affected the prolificacy trait of Dorper×Han crossbred sheep (F1) and Dorper×Han crossbred sheep (F3).

Key words: sheep; BMPR-1B gene; polymorphism

CLC Number:

S813.3

WU Cuiling, ZONG Xinglong, ZHAO Zhuo, ZHAO Yunhui, ZHAI Bo, ZHANG Mingxin, WANG Chunxin. Genetic Polymorphism of BMPR-1B Gene in Six Sheep Breeds[J]. , 2018, 45(4): 970-976.

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Genetic Polymorphism of BMPR-1B Gene in Six Sheep Breeds

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