米曲霉0-8固态发酵条件优化及其发酵产物对肉仔鸡日粮养分消化的影响

doi:10.16431/j.cnki.1671-7236.2017.11.011

Abstract

Abstract:

The high-yield protease was obtained by screening high-yield protease Aspergillus oryzae and optimizing its solid-state fermentation conditions, and then the effect of fermented product on nutrient digestion in broiler was studied. After the screening and re-screening of 8 different Aspergillus oryzae spores, a new Aspergillus oryzae strain 0-8 with high acid protease was obtained. The fermentation conditions of acid protease were optimized by single factor and orthogonal test experiment. The results showed that the optimum temperature was 30℃, the most suitable fermentation time was 66 h, the initial water content was 47.40%, the inoculation quantity was 1.5×10⁷ spores/mL, the content of bran was 80%, C/N was 1/3, the optimized vigor of acid protease reached 14 416.64 U/g, which was 157.02% higher than that before optimization. 500 mg/kg Aspergillus oryzae 0-8 fermented product was added in the broiler diet, and the mutrient digestibility of broiler were measured after 10 d of adaptation and 5 d of test period. The results of the nutrient digestion of broilers showed that feeding Aspergillus oryzae 0-8 fermentation increased the CP and energy digestibility of broiler in a certain degree compared to control group, but there's no significant difference (P>0.05). Dietary supplementation of Aspergillus oryzae fermentation was beneficial to the production of broilers, but its additive dose or feeding pattern should be further studied.

Key words: Aspergillus oryzae; solid state fermentation; acidic protease; broiler

CLC Number:

S816.7

MA Jian-qing, GUO Yan-li, ZHANG Tie-ying. Optimization of 0-8 Aspergillus oryzae Solid State Fermentation Conditions and the Effect of Fermented Product on Nutrient Digestion of Broiler[J]. , 2017, 44(11): 3179-3186.

References

[1] 陈力力. 血粉发酵生产中的微生物[J]. 肉类工业, 2003,7:228-229.
[2] 杨世平, 邱德全. 米曲霉产中性蛋白酶的适宜条件[J]. 广东海洋大学学报, 2005, 25(3):47-51.
[3] Rao M B, Tanksale A M, Ghatge M S, et al. Molecular and biotechnological aspects of microbial proteases[J]. Microbiology & Molecular Biology Reviews, 1998, 62(3):597-635.
[4] 姜锡瑞, 黄新宇. 酶制剂和食品工业[J]. 中国食品添加剂, 1998,3:33-36.
[5] 张红印, 郑晓冬, 席玙芳. 小麦面筋蛋白改性研究进展[J]. 中国食品学报, 2004, 4(1):93-97.
[6] 邓静, 吴华昌, 吴明霞,等. 米曲霉产蛋白酶条件的优化[J]. 中国酿造, 2008,12:51-53.
[7] 刘天蒙, 宋俊梅, 秦思思. 米曲霉固态发酵豆粕产蛋白酶条件的研究[J]. 现代农业科技, 2010,20:333-334.
[8] 张海龙. 米曲霉高活性蛋白酶菌株选育及酱油多菌种混合酵条件的研究[D]. 芜湖:安徽工程大学, 2011.
[9] 王朋朋, 尹清强, 王平,等. 米曲霉固态发酵生产蛋白酶、淀粉酶和纤维素酶的优化研究[A].饲料酶制剂的研究与应用[C]. 2009.
[10] 袁艳玲. 米曲霉产中性蛋白酶高产菌株的选育及其发酵条件优化[D]. 开封:河南大学, 2013.
[11] 杨世平, 邱德全. 米曲霉产中性蛋白酶的适宜条件[J]. 广东海洋大学学报, 2005, 25(3):47-51.
[12] 许尧兴, 李艳丽, 许少春,等. 米曲霉ZW-06在固态基质上产高活性中性蛋白酶发酵条件研究[J]. 浙江农业学报, 2008, 20(6):451-456.
[13] 李海梅, 马莺. 温度对曲霉产酶的影响[J]. 食品工业科技, 2004, 25(10):71-72.
[14] 费笛波, 钱玉英, 叶玲玲,等. 黑曲霉菌株6042饲用酸性蛋白酶的性质及其应用研究[J]. 浙江农业学报, 1997(6):300-304.
[15] Ashokkumar B, Kayalvizhi N, Gunasekaran P. Optimization of media for β-fructofuranosidase production by Aspergillus niger, in submerged and solid state fermentation[J]. Process Biochemistry, 2001, 36(12):1241-1247.
[16] Hong D, Burrows H, Adeola O. Addition of enzyme to starter and grower diets for ducks[J]. Poultry Science, 2002, 81(81):1842-1849.
[17] Wang Z R, Qiao S Y, Lu W Q, et al. Effects of enzyme supplementation on performance, nutrient digestibility, gastrointestinal morphology, and volatile fatty acid profiles in the hindgut of broilers fed wheat-based diets[J]. Poultry Science, 2005, 84(6):875-881.
[18] 王金全, 蔡辉益, 陈宝江,等. 小麦日粮中添加木聚糖酶对肉仔鸡生产性能、免疫、消化器官发育和血液代谢激素水平的影响[J]. 河北农业大学学报, 2005, 28(1):73-76.
[19] 罗定媛, 杨凤霞, 张利敏, 等. 小麦型日粮添加木聚糖酶对肉鸡生产性能和血液生化指标的影响[J]. 西北农林科技大学学报. 2009, 37(5):53-58.
[20] 许梓荣, 钱利纯, 孙建义. 高麦麸饲粮中添加β-葡聚糖酶、木聚糖酶和纤维素酶对肉鸡作用效果的研究[J]. 动物营养学报, 2000, 12(1):61-70.
[21] 周娟, 杨焕民, 姜宁, 等. 纤维素酶对肉仔鸡生产性能和营养物质利用的影响[J]. 黑龙江畜牧兽医, 2007,3:42-43.
[22] Yang F C, Lin I H. Production of acid protease using thin stillage from a rice-spirit distillery by Aspergillus niger[J]. Enzyme & Microbial Technology, 1998, 23(6):397-402.
[23] Kim S W, Knabe D A, Hong K J, et al. Use of carbohydrases in corn-soybean meal-based nursery diets[J]. Journal of Animal Science, 2003, 81(10):2496-2504.
[24] Pinheiro D F, Cruz V C, Sartori J R, et al. Effect of early feed restriction and enzyme supplementation on digestive enzyme activities in broilers[J]. Poultry Science, 2004, 83(83):1544-1550.
[25] Feng J. Effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers[J]. Poultry Science, 2007, 86(6):1149-1154.
[26] 孙春华. 米曲霉菌株的诱变选育及其制剂的应用研究[D]. 济南:山东农业大学, 2008.

Optimization of 0-8 Aspergillus oryzae Solid State Fermentation Conditions and the Effect of Fermented Product on Nutrient Digestion of Broiler

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WU Liting, LU Rui, LIU Banhong, BAO Hongduo, WANG Yongjuan, ZHOU Yan, WANG Ran, ZHANG Hui. Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2321-2332.
[2]	QIAO Hongxing, ZHANG Han, ZHAO Shengzhen, SUN Yanting, ZHANG Xiaojing, KANG Yu, BIAN Chuanzhou. Effects of Fermented Eucommia ulmoides Leaves on Production Performance, Antioxidant Properties and Immune Related Gene Expression in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1845-1854.
[3]	ZHANG Jinwu, WEI Zi, JI Yutong, WU Changxu, LI Zhenmin, WEI Yingyi, HU Tingjun, YU Meiling. Study on Oxidative Stress and Inflammatory Injury in Intestinal Tract of Yellow-feathered Chickens Induced by Clostridium perfringens Type A [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2069-2081.
[4]	LI Mengyuan, LIU Mengqian, KANG Jiajia, HUANG Ying, DUAN Yong, JIA Junjing, GE Changrong, CAO Weina. Effects of Guanidinoacetic Acid on Meat Quality and Abdominal Fat Deposition in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1385-1394.
[5]	LI Bin, SHI Zhengling, ZENG Ziyou, HUANG Yingyan, LI Lin, DU Zilai, JI Yifan, WANG Di, YU Jie, MEI Shaofeng. Effects of Chicory on Growth Performance,Slaughter Traits and Meat Quality of Mahuang Broilers with Different Gender [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 500-511.
[6]	LIN Xiajing, ZHENG Chuntian, XI Pengbin, CHENG Zhonggang, DING Fayuan, RUAN Dong, WANG Yibing, JIANG Shouqun. Dietary Threonine Requirement of Yellow-feathered Broilers Aged From 22 to 42 Days [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 134-142.
[7]	WU Yanan, SHI Yuxiang, ZHANG Yongying, LIU Guanhui, SONG Weiguang, ZHONG Cuihong, WANG Yongxia. Study on Nrf2 Activation Alleviating Heat Stress-Induced Oxidative Damage in Broiler Cardiomyocytes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3343-3352.
[8]	GAO Jiahao, QIAO Yanjie, LIAN Kexun, WANG Mengmeng, GU Xinli, SHAO Yongbin. Effects of Glycyrrhizic Acid on Immune Performance of Lipopolysaccharide-stressed Yellow Feather Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3419-3427.
[9]	LI Peiqi, KANG Jie, WANG Keyao, WANG Huimin, HAN Yufeng, WANG Tinghui, CUI Liang, ZHANG Jian, DENG Ruiqiang, DUAN Zhibian. Effect of Compound Traditional Chinese Medicine Water Extract on TGF-β1/Smad Pathway in Liver of Ascites Syndrom in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1860-1869.
[10]	WU Yuan, QIN Xin, DONG Yuanyang, HAN Miaomiao, MIAO Zhiqiang, WEI Qingyu, SONG Xianyi, SHEN Liyan, FENG Yan, LI Huifeng, LI Jianghui. Effects of Dietary Methionine on Growth Performance, Antioxidant Capacity and Stress Tolerance of Broilers Under High-density Feeding [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 913-923.
[11]	ZHU Cui, HUANG Kaiyong, LI Lina, WU Aiji, WU Peng, FU Fangmiao, LUO Yuanhua, ZHANG Huihua. Effects of Berberine on Organ Index, Antioxidant Capacity and Intestinal Immunity in Yellow-feathered Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 932-941.
[12]	ZHANG Lihuan, JIA Hao, ZHANG Ruonan, WANG Yanfei, LIU Xuan. Effects of Compound Probiotics on the Intestinal Microflora of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 488-500.
[13]	YU Qinping, HUANG Jingyu, ZHANG Cuiping, LIU Jing, HUANG Yuanbin, YU Ting, ZHANG Xiaoai, WANG Lei, CHEN Jinsheng, CHEN Wenqing, WANG Zhilin, CHEN Zhuang, RONG Ting. Effects of Barley Worm Meal on Growth Performance, Intestinal Structure and Microflora Composition of Yellow-feathered Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 548-558.
[14]	WANG Jiaxin, HAN Yunsheng, CAI Hongying, WEN Zhiguo, XU Xin, ZHAO Zitao, MENG Kun, YANG Peilong. Effects of Bacillus licheniformis on Growth Performance, Serum Biochemical Indexes and Intestinal Morphology of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4593-4603.
[15]	SHAN Chunqiao, ZHAI Hongxu, LI Juan, WANG Yan, YU Hongmin, WU Yiqi, LIU En, LIU Yan. Effects of Clostridium butyricum on Intestinal Short-chain Fatty Acid Content and Microflora Diversity of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4239-4251.