养殖鲤和锦鲤中的鲤浮肿病毒检测与分析

doi:10.16431/j.cnki.1671-7236.2017.10.036

Abstract

Abstract:

The carp edema virus (CEV) disease were caused by CEV, which may result in high losses in carp culture. In 2014, it had spread into China according to the report of the first CEV case confirmed in Beijing region. To investigate the epidemic status of CEV in farmed carps, select suitable method for CEV survey, and explore its pathogenic mechanism, from May to September in 2016, 13 samples were collected and detected CEV using Nest-PCR methods developed by Japanese researchers, and Nest-PCR methods and Real-time PCR method developed by UK researchers. The results showed that 3 koi samples and 2 common carp samples of CEV positive were found. Real-time PCR and Nest-PCR could be well used to detect CEV in koi samples, but Nest-PCR could not detect CEV well in common carp. In positive samples, there were no clinical signs and death in case 3 and case 10 of farmed fish, and case 3 harboured higher copy numbers of CEV-specific DNA than that of case 7, case 8 and case 13 with signs of disease. Based on the above information, surveillance programs need to carry out urgently to avoid further spread of CEV according to the epidemic status of CEV. And Real-time PCR method was more suitable for CEV survey than Nest-PCR method. In addition, the farmed carps did not always occurred CEV disease in optimum temperature when the farmed fish carried CEV or the CEV mass reproduction in fish body. Therefore, the environmental stress was the crucial factor leading to the occurrence of the CEV disease, avoiding environmental stress was an effective measure to reduce the occurrence of the CEV disease.

Key words: carp; koi; carp edema virus disease

CLC Number:

S941.41

WANG Xiao-liang, XU Li-pu, WANG Shu, WANG Jing-bo, CAO Huan, ZHANG Wen, JIA Li. Detection and Analysis of Carp Edema Virus (CEV) in Farmed Carp and Koi[J]. , 2017, 44(10): 3084-3089.

References

[1] Ona S I, Nagai N. A histopathological study on Juvenile colorcarp, Cyprinus carpio showing edema[J]. Fish Pathology, 1986,21(3):167-175.
[2] Miyazaki T,Isshiki T,Katsuyuki H. Histopathological and electron microscopy studies on sleepy disease of koi Cyprinus carpio koi in Japan[J]. Diseases of Aquatic Organisms, 2005, 65(3):197-207.
[3] Murakami Y, Shitanaka M, Toshida S, et al. Studies on mass mortality of Juvenile carp:About mass mortality showing edema[J].Bull Hiroshima Fresh Water Fish,1976,1:19-33.
[4] Tomasec I I,Fijan N N. The etiology of infectious dropsy of carp[J]. Annals of the New York Academy of Sciences,1965,126(1):606-615.
[5] Oyamatsu T, Hata N, Yamada K, et al. An etiological study on mass mortality of cultured colorcarp juveniles showing edema[J].Fish Pathology,1997,32(2):81-88.
[6] Way K, Stone D. Emergence of carp edema virus-like (CEV-like) disease in the UK[J].CEFAS Finfish News,2013,15:32-34.
[7] Haenen O, Way K, Stone D,et al."Koi sleepy disease" found for the first time in koi carps in the Netherlands[J].Tijdschr Dierqeneeskd,2014,139(4):26-29.
[8] Matras M, Borzym E, Stone D, et al. Carp edema virus in Polish aquaculture-evidence of significant sequence divergence and a new lineage in common carp Cyprinus carp (L.)[J].Journal of Fish Disease,2017, 40(3):319-325.
[9] Lewisch E, Gorgoglione B, Way K, et al. Carp edenma virus/kio sleepy disease:An emerging disease in Central-East Europe[J]. Transboundary and Emerging Diseases, 2014,62(1):6-12.
[10] Jung-Schroers V, Adamek M, Teitge F, et al. Another potential carp killer?:Carp edema virus disease in Germany[J]. BMC Veterinary Research, 2015,11:114-117.
[11] 徐立蒲,王小亮,张文,等.养殖锦鲤鲤鱼浮肿病的检测和鉴定[J].中国畜牧兽医,2017,44(2):613-618.
[12] Oyamatsu T, Matoyama H, Yamamoto K, et al. A trial for the detection of carp edema virus by using polymerase chain reaction[J].Suisanzoshoku, 1997,45:247-252.
[13] Adamek M, Jung-Schroers V, Hellmann J, et al. Concentration of carp edema virus (CEV) DNA in koi tissues affected by koi sleepy disease (KSD)[J].Diseases of Aquatic Organisms, 2016,119(3):245-251.
[14] 农业部渔业渔政管理局,全国水产技术推广总站.2014年我国水生动物重要疫病病情分析[M].北京:海洋出版社, 2016.
[15] 农业部渔业渔政管理局.2014年中国渔业统计年鉴[M].北京:中国农业出版社,2014.
[16] Amita K, Oe M, Matoyama H, et al.A survey of koi herpesvirus and carp edema virus in colorcarp cultured in Niigata prefecture,Japan[J].Fish Pathology,2002,37:197-198.

Detection and Analysis of Carp Edema Virus (CEV) in Farmed Carp and Koi

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