牛源产志贺毒素大肠杆菌分离株的毒力基因分布和遗传进化分析

doi:10.16431/j.cnki.1671-7236.2017.10.008

Abstract

Abstract:

This study was aimed to understand the relationship of virulence gene distribution and genetic evolution between cattle originated Shiga toxin-producing Escherichia coli (STEC) and human originated enterohaemorrhagic Escherichia coli (EHEC) O157. This experiment collected 18 strains STEC in a dairy farm from Jiangsu province and 9 STEC reference strains (human, sheep, swine and avian), according to the method of U.S. Centers for Disease Prevention and Control Center (PulseNet), using the XbaⅠ enzyme digestion and pulsed field gel electrophoresis (PFGE) analysis, virulence genes were detected in some STEC isolates. The virulence gene distribution of O157 from different origin was remarkably different. The cattle originated STEC O157 and the human originated EHEC O157:H7 (EDL933W) had the most similar virulence gene distribution. In contrast, virulence genes were lack in cattle STEC O18 and O26, even though the cattle STEC O18 and O26 had the similar genotype as human EHEC O157:H7 (EDL933W). PFGE of Xba Ⅰ digested chromosomal DNA from 27 isolates of STEC exhibited 22 profiles. In general,the Dice coefficients of different originated STEC ranged from 72% to 100%.Cattle STEC O157 had a high similarity with two strains of human originated EHEC O157, while a low similarity was demonstrated between cattle STEC O157 and STEC O157 of swine and avian. The Dice coefficients of the cattle STEC O157 and the two strains of human EHEC O157 ranged from 83% to 95%. The Dice coefficients of cattle STEC O26 (Ⅶ,Ⅷ) and the two strains of human EHEC O157 were more than 82%. Therefore, it was concluded that the cattle STEC O157 and human EHEC O157 had a closer relationship in terms of virulence gene distribution and in genetic evolution.

Key words: Shiga toxin-producing Escherichia coli(STEC); enterohaemorrhagic Escherichia coli(EHEC); virulence gene distribution; pulsed field gel electrophoresis; gene evolution

CLC Number:

S852.61⁺2

XUE Tao, LI Li, GAO Qing-qing, CHEN Xian-liang, LI Tian-tian, QU Xin-qin, GAO Song. Study on the Virulence Gene Distribution and Genetic Evolution of Cattle Shiga Toxin-producing Escherichia coli Isolates[J]. , 2017, 44(10): 2878-2885.

References

[1] Scallan E, Hoekstra R M, Angulo F J, et al. Foodborne illness acquired in the united states-major pathogens[J].Emerg Infect Dis,2011,17(1):7-15.
[2] Luna-Gierke R, Griffin P, Gould L, et al. Outbreaks of non-O157 Shiga toxin-producing Escherichia coli infection:USA[J].Epidemiol Infect,2014,142(11):2270-2280.
[3] Shridhar P B, Siepker C, Noll L W, et al. Shiga toxin subtypes of non-O157Escherichia coli serogroups isolated from cattle feces[J].Front Cell Infect Microbiol,2017,7:121.
[4] Schimmer B, Nygard K, Eriksen H M, et al. Outbreak of haemolytic uraemic syndrome in Norway caused by stx2-posetive Escherichia coli O103:H25 traced to cured mutton sausages[J]. BMC Infect Dis, 2008, 8:41.
[5] 白莉,郭云昌,董银苹,等.我国食品中大肠埃希菌O157耐药及PFGE分子分型特征分析.[J].中国食品卫生杂志,2014,2(5):422-427.
[6] Law D. Virulence factors of Escherichia coli O157 and other Shiga toxin-producing E.coli[J].Appl Microbiol,2000:88(5):729-745.
[7] Dong H J,Lee S,Kim W,et al.Prevalence,virulence potential and pulsed-field gel electrophoresis profiling of Shiga toxin-producing Escherichia coli strains from cattle[J].Gut Pathogens,2017,9:22.
[8] Miko A, Delannoy S, Fach P, et al. Genotypes and virulence characteristics of Shiga toxin-producing Escherichia coli O104 strains from different origins and sources[J].Int J Med Microbiol, 2013, 303(8):410-412.
[9] Otero V, Rodriguez-Calleja J M, Otero A, et al. Genetic characterization of atypical enteropathogenic Escherichia coli isolates from ewes milk,sheep farm environments,and humans by multilocus sequence typing and pulsed-field gel electrophoresis[J]. Appl Environ Microbiol,2013,79(19):5864-5869.
[10] 薛涛, 顾丛丛, 高崧,等. 江苏某奶牛场健康奶牛体内产志贺毒素大肠杆菌的分离鉴定及其对小鼠致病性的研究[J]. 畜牧兽医学报, 2011, 42(6):88-96.
[11] 薛涛, 顾丛丛, 高崧,等. 江苏某地健康绵羊群产志贺毒素大肠杆菌体内分离株的分子流行病学及致病力[J]. 微生物学报, 2011, 51(5):115-122.
[12] El-Adhami W, Roberts L, Vickery A, et al. Epidemiological analysis of a methicillin-resistant Staphylococcus aureus outbreak using restriction fragment length polymorphisms of genomic DNA[J]. Gen Microbiol, 1991, 137(12):2713-2720.
[13] Bibbal D, Loukiadis E, Kerouredan M, et al. Prevalence of carriage of Shiga toxin-producing Escherichia coli serotypes O157:H7,O26:H11,O103:H2,O111:H8,and O145:H28 among slaughtered adult cattle in France[J]. Appl Environ Microbiol, 2015, 81:1397-1405.
[14] Boerlin P, Mcewen S A,Boerlin P F. Association between virulence factors of Shiga toxin-producing Escherichia coli and disease in human[J]. Clin Microbiol,1999, 37:497-503.
[15] Shen J, Rump L, Ju W, et al. Virulence characterization of non-O157 Shiga toxin-producing Escherichia coli isolates from food,humans and animals[J].Food Microbiol,2015,50:20-27.
[16] 顾玲, 周璐, 董晨,等. 江苏省肠出血性大肠杆菌O157:H7 PFGE分子分型研究[J].中国卫生检验杂志, 2013, 23(3):565-568.
[17] Tenover F C, Arbeit R D, Goering R V, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis:Criteria for bacterial strain typing[J]. Clin Microbiol, 1995, 33(9):2233-2239.
[18] Talon D, Cailleaux V, Thouverez M, et al. Discriminatory power and usefulness of pulsed-field gel electrophoresis in epidemiological studies of pseudomonas aeruginosa[J]. Hosp Infect, 1996, 32(2):135-145.
[19] Severi E, Vial F, Peron E, et al.Community wide outbreaks of haemolytic uraemic syndrome associated with Shiga toxin producing Escherichia coli O26 in Italy and Romania:A new challenge for the European Union[J].Euro Surveill,2016,21(49):30420.

Study on the Virulence Gene Distribution and Genetic Evolution of Cattle Shiga Toxin-producing Escherichia coli Isolates

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