小檗碱对禽源大肠杆菌抑菌作用及耐药消除作用的转录组学分析

doi:10.16431/j.cnki.1671-7236.2017.05.036

Abstract

Abstract:

In order to explore the mechanism of traditional Chinese medicine eliminated the antibiotic resistance of Escherichia coli, with berberine as drug treatment, the antibiotic-resistant Escherichia coli isolated from clinical isolates was cultured in LB broth with a concentration of 250 μg/mL (1/2 MIC) of berberine, and passaged for 3 generations every 24 h. The MIC of the mutant was determined by microplate method. The MIC of the mutant was determined by photolithography. The MIC of levofloxacin was reduced from 16 to 8 μg/mL, and the drug resistance to levofloxacin was eliminated. In order to understand the molecular mechanism of berberine's effect on Escherichia coli, the gene expression level of avian Escherichia coli was analyzed by transcriptome sequencing method before and after drug resistance elimination. The results showed that the expression level of 45 genes was significantly changed after treated with berberine, among which 30 genes were up-regulated and 15 genes were down-regulated. The enrichment analysis of GO function and enrichment analysis of KEGG metabolic pathways showed that up-regulated genes were mainly included the synthesis of tryptophan, the binding of pyridoxal phosphate,transketolase.Down-regulation of multiple genes were in the two-component system, the ybjG of the undecylpodophosphate phosphatase (UppP) gene and acyl-CoA dehydrogenase synthesis related genes.Escherichia coli in vivo enzyme activity might be the main mechanism of inhibition of berberine.Decreased activity of the multi-drug resistant efflux pump and the changes of Escherichia coli cell membrane and cell wall might be the main mechanisms for the elimination of berberine resistance.

Key words: Escherichia coli; berberine; drug resistance elimination; transcriptome; Real-time quantitative PCR

CLC Number:

S858.3

ZHANG Shi-lei, ZHAI Xiang-he, WANG Chun-guang, CHEN Jian, JIA Ze, ZHANG Tie. Study on Antimicrobial Effect of Berberine Sulfate on Avian Escherichia coli and Elimination of Levofloxacin Resistance Based on Transcriptome Sequencing[J]. , 2017, 44(5): 1518-1525.

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Study on Antimicrobial Effect of Berberine Sulfate on Avian Escherichia coli and Elimination of Levofloxacin Resistance Based on Transcriptome Sequencing

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