梅山猪与杜洛克猪中等卵泡差异表达基因的鉴定

doi:10.16431/j.cnki.1671-7236.2017.02.029

Abstract

Abstract:

To reveal the molecular mechanism involved in different number of ovulation between hyperprolific and ordinary sows, forward and reverse subtracted cDNA libraries were constructed to screen differentially expressed genes in medium ovarian follicles at 4th day during follicular phase of estrous cycle between Meishan and Duroc sows. The differentially expressed genes selected were demonstrated by Real-time PCR and cluster analysis was performed through DAVID. The results showed that a total of 148 and 75 non-redundant ESTs were isolated from the SSH library of Meishan and Duroc sows M2 follicles, including 125 and 60 known genes, respectively. The results of Real-time PCR were consistent with the screening results. GO analysis indicated that these genes were involved in regulation of metabolic process, cell cycle, biosynthetic process, intracellular transport, steroid hormone receptor and stimulus. KEGG pathway analysis defined 6 genes involved in TGF-beta signaling pathway, 5 genes in oocyte meiosis pathway, 7 genes in steroid hormone receptor signaling pathway. Genes controlling TGF-beta signaling pathway were considered to play an important role in regulating follicle development. The research would be helpful for further studying the follicular development and the genetic mechanism on reproductive traits.

Key words: differentially expressed genes; ovarian follicle; Meishan sow; Duroc sow

CLC Number:

S814.8

ZHAO Zhi-chao, HUANG Tao, YANG Fei, LIU Li-juan, SUN Jing-li, WANG Xiao-yan, SONG Cheng-yi, LI Da-quan. Identification of Differentially Expressed Genes in Medium-sized Ovarian Follicles between Meishan and Duroc Sows[J]. , 2017, 44(2): 505-513.

References

[1] Miller A T, Picton H M, Craigon J, et al. Follicle dynamics and aromatase activity in high-ovulating Meishan sows and in Large-White hybrid contemporaries[J]. Biol Reprod,1998, 58(6):1372-1378.
[2] Yen H W, Ford J, Zimmerman D, et al. Follicular development and maturation in gilts selected for an index of high ovulation rate and high prenatal survival[J]. J Anim Sci, 2005, 83(1):130-135.
[3] Wolter B F, Ellis M, Curtis S E, et al. Group size and floor-space allowance can affect weanling-pig performance[J]. J Anim Sci, 2000, 78(8): 2062-2067.
[4] 杨公社.猪生产学[M].北京:中国农业出版社,2002.
[5] Bazer F, Thatcher W, Martinat-Botte F, et al. Sexual maturation and morphological development of the reproductive tract in Large White and prolific Chinese Meishan pigs[J]. J Reprod Fertil, 1988, 83(2):723-728.
[6] Bolet G, Botte F M, Locatelli A, et al. Components of prolificacy in hyperprolific Large White sows compared with the Meishan and Large White breeds[J]. Genet Sel Evol, 1986,18(3):333-342.
[7] 李炳坦,赵书广,郭传甲.养猪生产技术手册[M].第2版.北京:中国农业出版社, 2004.
[8] Davis K L, Robison O W,Toelle V D. Breed differences in uterine and ovarian measurements in gestating swine[J]. J Anim Sci,1987, 65(3):685-691.
[9] Foxcroft G R, Hunter M G. Basic physiology of follicular maturation in the pig[J]. J Reprod FertilSuppl,1985,33:1-19.
[10] Yen H W. Follicular development, maturation and atresia during the estrous cycle in gilts expressing high and low ovulation rates[D].Lincoln:University of Nebraska,1999.
[11] Yen H W, Ford J J, Zimmerman D R,et al. Follicular development and maturation in gilts selected for an index of high ovulation rate and high prenatal survival[J]. J Anim Sci, 2005, 83(1):130-135.
[12] Grado-Ahuir J A, Aad P Y, Ranzenigo G, et al. Microarray analysis of insulin-like growth factor-Ⅰ-induced changes in messenger ribonucleic acid expression in cultured porcine granulose cells: Possible role of insulin-like growth factor-Ⅰ inangiogenesis[J]. J Anim Sci, 2009, 87(6):1921-1933.
[13] Paradis F, Moore H S, Pasternak J A, et al. Pig preovulatory oocytes modulate cumulus cell protein and gene expression in vitro[J]. Mol Cell Endocrinol,2010, 320(1-2): 87-96.
[14] Toms D, Tsoi S, Dobrinsky J, et al. The effects of glial cell line-derived neurotrophic factor on the in vitro matured porcine oocyte transcriptome[J]. Mol Reprod Dev,2014, 81(3): 217-229.
[15] Shi Y, Massagué J. Mechanisms of TGF-β signaling from cell membrane to the nucleus[J]. Cell, 2003, 113(6):685-700.
[16] Onichtchouk D, Chen Y G, Dosch R, et al. Silencing of TGF-β signalling by the pseudoreceptor BAMBI[J]. Nature, 1999,401(6752):480-485.
[17] 杨飞,黄涛,赵志超,等. 杜洛克与梅山猪大卵泡消减文库的构建[J]. 石河子大学学报, 2014, 32(5):543-549.
[18] Sun X, Mei S, Tao H, et al. Microarray profiling for differential gene expression in PMSG-hCG stimulated preovulatory ovarian follicles of Chinese Taihu and Large White sows[J]. BMC Genomics, 2011,12:111-120.
[19] Zhang Q, Sun H, Jiang Y, et al. microRNA-181a suppresses mouse granulosa cell proliferation by targeting activin receptor ⅡA[J]. PLoS One, 2013, 8(3):e59667.
[20] Li Q, Pangas S A, Jorgez C J, et al. Redundant roles of SMAD2 and SMAD3 in ovarian granulosa cells in vivo[J]. Mol Cell Biol, 2008, 28(23):7001-7011.
[21] 徐梦思,黄涛,刘丽娟,等.猪SKP1基因的克隆及其在梅山猪与杜洛克猪卵泡中的表达研究[J]. 中国畜牧兽医,2015, 42(7):1640-1646.
[22] De S, Kline D. Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation[J]. BMC Dev Biol, 2013, 13:1-15.
[23] De S, Marcinkiewicz J L, Vijayaraghavan S, et al. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development[J]. BMC Research Notes, 2012, 5:57.
[24] 刘丽娟,鲁慧文,杨飞,等.YWHAG基因CDS的克隆及其在梅山与杜洛克猪卵泡发育中的差异表达研究[J]. 畜牧兽医学报,2014, 45(3):489-493.
[25] Lanzino M, De Amicis F, McPhaul M, et al. Endogenous coactivator ARA70 interacts with estrogen receptor alpha (ERalpha) and modulates the functional ERalpha/androgen receptor interplay in MCF-7 cells[J]. J Biol Chem, 2005, 280(21):20421-20430.
[26] Gao T, Brantley K, Bolu E, et al. RFG (ARA70, ELE1) interacts with the human androgen receptor in a ligand-dependent fashion, but functions only weakly as a coactivator in cotransfection assays[J]. Molecular Endocrinology, 1999, 13(10):1645-1656.
[27] Ting H J, Bao B Y, Hsu C L, et al. Androgen-receptor coregulators mediate the suppressive effect of androgen signals on vitamin D receptor activity[J]. Endocrine, 2005, 26(1):1-9.
[28] Moore J M, Galicia S J, McReynolds A C, et al. Quantitative proteomics of the thyroid hormone receptor-coregulator interactions[J]. J Biol Chem, 2004, 279(26):27584-27590.
[29] Viswakarma N, Jia Y, Bai L, et al.Coactivators in PPAR-regulated gene expression[J]. PPAR Research, 2010, 2010:250126.

Identification of Differentially Expressed Genes in Medium-sized Ovarian Follicles between Meishan and Duroc Sows

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 8

Recommended Articles

Metrics

Comments

[1]	YANG Shuai, SHI Mingyue, LI Wenxia, YUN Jiale, SUN Di, LU Chang, YANG Yang, CAI Chunbo, GAO Pengfei, GUO Xiaohong, LI Bugao, CAO Guoqing. Screening and Expression Analysis of Genes Related to Porcine Intramuscular Fat Deposition [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1651-1661.
[2]	TIAN Wenjie, WANG Dandan, WANG Shengnan, MA Yuehui, JIANG Lin, SONG Ziyi. Transcriptome Analysis of Porcine Kidney with ZBED6 Gene Deletion [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1662-1670.
[3]	CHEN Ting, LU Shengsheng, YANG Lili, LI Tianbao, ZHANG Min, JIANG Mingsheng. Based on Transcriptome Sequencing to Explore the Relationship Between Growth and Development Genes of Guangxi Ma Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3901-3908.
[4]	HAN Haoyuan, LI Wantao, LEI Chuzhao. Identification and Analysis of mRNA and microRNA Expression of Testis Tissue in Dezhou and Biyang Donkeys [J]. , 2019, 46(8): 2371-2380.
[5]	WU Xianfeng, LI Wenyang, LIU Yuan, ZHANG Ying, HUANG Qinlou. Comparative Transcriptome Analysis of Ovary Tissues in Fuqing and Daiyun Goats During Summer Estrus [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3277-3288.
[6]	LIANG Wan, ZHOU Danna, PENG Zhong, YANG Keli, DUAN Zhengying, GUO Rui, LIU Wei, WU Bin, CHEN Wenjie, MENG Li, TIAN Yongxiang. Application of Transcriptome Sequencing Technology in Porcine Reproductive and Respiratory Syndrome Virus Research [J]. , 2019, 46(1): 37-44.
[7]	DI Ran;CHU Ming-xing . High Throughput Screening Technologies of Differentially Expressed Genes and their Application in Domestic Animals [J]. , 2011, 38(1): 144-147.
[8]	YUE Yang;WANG Dong;HAO Hai-sheng;DU Wei-hua;ZHAO Xue-ming;ZHU Hua-bin;LU Yong-qiang. Research Progress on Detection and Assay of Differentially Expressed Genes from Subtractive cDNA Libraries [J]. , 2010, 37(10): 100-104.