3种中药及其提取物体外抗猪繁殖与呼吸综合征病毒作用的研究

doi:10.16431/j.cnki.1671-7236.2016.10.032

Abstract

Abstract:

In order to figure out the antiviral effects of Radix Isatidis,Astragalus herb,Artemisia annua and their polysaccharides on porcine reproductive and respiratory syndrome virus (PRRSV) in vitro,the antiviral activity of the three Chinese herbal medicine and their polysaccharides,Isatis root polysaccharide (IRPS),Astragalus polysaccharides (APS) and artemisinin maximum were evaluated by adding to Marc-145 cell cultured with PRRSV respectively.The results showed that the safety concentrations of IRPS,APS and artemisinin to Marc-145 cells were 0.03,0.03 and 0.06 mg/mL;The safety concentrations of Radix Isatidis,Astragalus herb,Artemisia annua to Marc-145 cells were 6.25,6.25 and 3.13 mg/mL.Almost all the cells (about 100%) were protected from PRRSV when Astragalus herb were added early to PRRSV,while IRPS had the same effect on PRRSV among three extracts with 48.03% cells were protect and artemisinin could not protect cells when the concentration was lower than 0.16 mg/mL.IRPS had no effect on PRRSV while APS and artemisinin got a protection rate for cells at about 20% when simultaneously added with PRRSV,under the concentration of 0.32 mg/mL.In terms of simultaneously added with PRRSV,Chinese herb Astragalus could exterminate the virus directly with its protect cells coming to 100% and more.Artemisia annua could promote the growth of cells but its stability was weak.Its protection rate decreased as its concentration reducing.In the experiment of exploring the antivirus effect of infected cells,polysaccharides of these three herbs had nearly the same effect.When in a concentration of 0.01 mg/mL,IRPS,APS and artemisinin respectively protected 24.57%,24.30% and 26.20% cells.However,the protection rate to cells of the three herbs themselves was respectively up to 100% or more.In conclusion,in the extracts of the three herbs,APS had the best antiviral effect,followed by artemisinin and IRPS,and in the single herbal medicines,Astragalus was the best and Artemisia annua was worst.Therefore,Astragalus and APS had the best antiviral effect on PRRSV.It needed more research on Astragalus and APS,and try to use them as antiviral drug in clinical application.

Key words: PRRSV; Isatis root polysaccharide; Astragalus polysaccharides; artemisinin

CLC Number:

S858.28

LIU Ying, DING Du-wei, GAO Qiu-wei, LIANG Jia-qi, XIAN Qiong-zhen, FENG Jun, WANG Bing-yun. Antiviral Effects of Three Chinese Herbal Medicine and Their Polysaccharides on Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in vitro[J]. , 2016, 43(10): 2730-2735.

References

[1] 蔡宝祥.我国高致病性猪繁殖与呼吸综合征研究进展[J].畜牧与兽医,2010,10:1-4.
[2] 郭宝清,陈章水,刘文兴,等.从疑似PRRS流产胎儿分离PRRSV的研究[J].中国畜禽传染病,1996,2:1-5.
[3] 王艳丰,张丁华,曹智高,等.高致病性猪繁殖与呼吸综合征协同感染及防制研究进展[J].中国畜牧兽医,2014,41(5):235-239.
[4] 母安雄,濮文正,蒋建一,等.4种猪生殖和呼吸综合征灭活疫苗免疫效果比较[J].中国兽医杂志,2003,9:18-19.
[5] 王珍芹,曲志娜.猪繁殖与呼吸道综合征免疫预防进展[J].中国动物检疫,2005,12:37-38.
[6] 邹宝仁.中药在兽医临床中应用的研究进展[J].黑龙江科技信息,2014,24:51.
[7] 史利卿.抗呼吸道病毒中药的实验研究述评[J].中国中医基础医学杂志,1998,12:55-57.
[8] 邢娜.抗猪繁殖与呼吸综合征病毒(PRRSV)中药高效成分的体外筛选及其抗病毒机制的研究[D].太谷:山西农业大学,2015.
[9] 布玮玮.河南省PRRSV分子流行病学调查及体外抗病毒中药单体的筛选[D].洛阳:河南科技大学,2014.
[10] 陈志胜,王丙云,冼琼珍等.三种中药提取物体外抗猪繁殖与呼吸综合征病毒的作用[J].中国兽药杂志,2014,48(8):40-43.
[11] 朱婷,尹溽纹,张贵强,等.板蓝根多糖提取工艺及对活性成分影响的研究[J].中国新药杂志,2013,22(12):1390-1395.
[12] 王淑萍,李晓静,张桂珍.黄芪多糖提取分离纯化工艺的优化研究[J].分子科学学报,2008,1:60-64.
[13] 冼琼珍,王丙云,丘顺寿,等.10种中药体外抗猪繁殖与呼吸综合征病毒的作用研究[J].黑龙江畜牧兽医,2014,1:133-136.
[14] 冼琼珍,王丙云,伍盛鋆,等.16种中药体外抗猪繁殖与呼吸综合征病毒作用的研究[J].中国畜牧兽医,2014,41(2):245-249.
[15] 麻延峰,王宏艳,傅春泉,等.板蓝根多糖对PRRSV疫苗的免疫增强试验[J].家畜生态学报,2011,6:74-76.
[16] 张红英.3种植物多糖抗猪蓝耳病病毒及免疫增强作用研究[D].郑州:河南农业大学,2007.
[17] 杜继红,朱华,崔小七,等.板蓝根颗粒对鸡传染性法氏囊病的防制效果研究[J].中国畜牧兽医,2014,41(6):236-240.
[18] 张小梅.黄芪多糖的免疫调节作用及抗肿瘤作用研究进展[J].大连大学学报,2003,6:101-104.
[19] 王学兵,张红英,崔保安,等.板蓝根多糖对PRRSV的体外抗病毒试验[J].西北农业学报,2009,1:198-200.
[20] 袁彬,彭德文.多糖在畜牧兽医中的应用[J].北京农业,2013,12:120.
[21] 李宏全,段县平,马海利,等.黄芪多糖提高鸡抗氧化作用对免疫功能的影响[J].山西农业大学学报,自然科学版,2002,1:78-81.
[22] 蔡博莹.青蒿素的研究进展[J].科学中国人,2015,35:55.

Antiviral Effects of Three Chinese Herbal Medicine and Their Polysaccharides on Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in vitro

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[2]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[3]	LI Huawei, GUO Kewei, WANG Xuying, ZHANG Xiaoyu, LU Ziwei, LI Xinfeng, MA Hui, HOU Wenjing. Eukaryotic Expression and Subcellular Localization of Nonstructural Protein 7 of Porcine Reproductive and Respiratory Syndrome Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1160-1168.
[4]	SHAO Baihui, JIANG Dongjun, XIE Yimeng, LIU Siyu, SHAO Ziyi, ZHANG Zecai, JI Hong, ZHU Zhanbo. Molecular Docking Prediction of Artemisinin and NS5B Protein of Bovine Viral Diarrhea Virus and Its Antiviral Effect [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3581-3588.
[5]	LIU Qingqing, WU Peng, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu, XIAO Chencheng. Construction of Recombinant Adenovirus Expressing Porcine Reproductive and Respiratory Syndrome Virus N Protein and Its Immunogenicity Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1024-1031.
[6]	WANG Zhaoli, YANG Siyu, WU Yanmei, SONG Xiaoxiao, MU Xiang, ZHANG Tao. Effects of PRRSV-LPS Treatment on Neutrophil Adhesion to Porcine Pulmonary Microvascular Endothelial Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 471-479.
[7]	SUN Lu, ZHANG Jing, YIN Miao, CHENG Yaodie, DAI Kun, WU Qian, ZHAO Hong, CHEN Xiwen. Study on the Antibacterial and Anti-PRRSV Activities of Four Heat Clearing and Detoxifying Compound Chinese Herbal Medicine in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 746-754.
[8]	CHU Pinpin, JIANG Zhiyong, LI Chunling, GOU Hongchao, LI Yan, CAI Rujian. Antiviral Effect of Silver Nanoparticles on Porcine Reproductive and Respiratory Syndrome Virus in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4356-4363.
[9]	DENG Jiping, CHEN Zhilong, GUO Songyangnian, ZHANG Kun, XU Daojun, YANG Qing, YUAN Anwen. Genetic Evolution Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolated from Hunan Province [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3795-3802.
[10]	LIU Hanyang, ZHANG Yi, XIAO Ran, CHEN Bin, WU Yongsheng, SU Zhipeng, LI Yupeng, PEI Chaoxin, XU Zhenying, ZHU Jiawen. Molecular Epidemiological Investigation on ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in Sichuan in 2018 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1172-1182.
[11]	ZHOU Shumian, CAO Mixia, HU Tingjun. A Study on Effect of Anti-PRRSV of n-butanol Part of Flavonoids from Polygonum hydropiper L. in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 605-611.
[12]	ZHANG Xiyi, WEN Guilan, GUAN Guodan, CHEN Guang, TIAN Lang, YANG Baiqi. Cloning and Genetic Variation Analysis of NSP2 Gene of Porcine Reproductive and Respiratory Syndrome Virus Guizhou Strain GZ-R [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3659-3666.
[13]	DENG Tongwei, XUE Yanan, LU Jianzhou, XIA Yanxun, CHEN Lulu, GUO Yiwen, PENG Liming, JIANG Zenghai, QIAO Hongxing, XU Yaohui, ZHANG Xiaozhan. Diagnosis and Pathogen Analysis of Mixed Infection of Porcine Reproductive and Respiratory Syndrome Virus,Porcine Circovirus Type 2 and Haemophilus parasuis [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3401-3409.
[14]	HAN Yiqing, JI Xun, CHEN Chunqin, LIU Mingjiang, LI Jingui. Study on the Metabolism of Artemisinin in Chickens by LC-MS/MS [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2778-2786.
[15]	ZHANG Hailong, CHEN Shengnan, XIE Bo, LI Li, HUANG Liangzong. Diagnosis of Mixed Infection of Porcine Reproductive and Respiratory Syndrome Virus and Haemophilus parasuis [J]. , 2019, 46(6): 1856-1864.