重组减毒沙门氏菌ΔcrpC79-13(pcDNA3-HN)在雏鸡体内主要组织器官定植情况检测

doi:10.16431/j.cnki.1671-7236.2016.09.036

Abstract

Abstract:

The present study was designed to detect ΔcrpC79-13(pcDNA3-HN) which was a recombinational attenuated Salmonella embedded status in chicks major organs,120 one-day chicks were randomly assigned into 3 treatments according to a single factorial arrangement,which were immunized by ΔcrpC79-13,recombinational ΔcrpC79-13(pcDNA3-HN) and PBS,respectively.After immunization for 4,24,48,72,96,120,144 and 168 h,the embedded status of ΔcrpC79-13and recombinational ΔcrpC79-13(pcDNA3-HN) was detected in the heart,liver,spleen,cecum and blood of chicks.The results showed that the amount of attenuated immune Salmonella ΔcrpC79-13 reached a peak in the blood within 24 h,blood bacterial population decline in 24 to 48 h, number of bacteria in the blood rose at 48 to 96 h and reached another peak in 96 h,and the bacteria of the blood fell at 96 to 168 h.In the heart,liver,spleen and cecum,the number of bacteria in these organs at 4 to 72 h had an upward trend and reached a peak at 72 h,viscera bacterial population declined at 72 to 96 h,and the count rose at 96 to 120 h and reached a peak at 120 h,then the number of bacteria decreased.It was similar to the change in each viscera strain of group ΔcrpC79-13(pcDNA3-HN).These data indicated that both of the recombinational bacterial strains ΔcrpC79-13(pcDNA3-HN) and parental bacterial strain ΔcrpC79-13 could be embedded in a series of organ of chicks such as heart,liver,spleen,and cecum.Meanwhile,there was a consistent declining trend of bacterial strain amount in each viscus of chicks.

Key words: ΔcrpC79-13; ΔcrpC79-13(pcDNA3-HN); chick; colonization

CLC Number:

S852.61

JIN San-jun, DONG Jia-qi, WU Hong-zhi, SONG Jian-lou, WANG Chuan-qi, GUO Zhao-zhou, DIAO Xin-ping, CHENG Xiang-chao. Colonization Detection of Recombinant Attenuated Salmonella ΔcrpC79-13 (pcDNA3-HN) in Major Organs of Chicks[J]. , 2016, 43(9): 2468-2473.

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Colonization Detection of Recombinant Attenuated Salmonella ΔcrpC79-13 (pcDNA3-HN) in Major Organs of Chicks

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