马鹿Zfx/Zfy基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2016.09.008

Abstract

Abstract:

Specific primers were designed according to the mRNA sequence of Bos taurus Zfx/Zfy genes provided in GenBank.Zfx/Zfy sequences of Cervus elaphus were cloned and analyzed.The results showed that the cloned Zfx gene sequence of Cervus elaphus was 2 115 bp (GenBank accession No:KP257294.1) that encoded 696 amino acids,the cloned Zfy gene sequence was 2 406 bp (GenBank accession No:KU041539) that encoded 801 amino acids.The phylogenetic trees of Zfx and Zfy amino acid were obtained using the Neighbor-Joining method of DNAStar software.Analysis showed that Zfx of Cervus elaphus was closely related to Homo sapiens,compared with Bison,Pantholops hodgosoniiand,Panthera tigris,Felis catus,Bos taurus,Canis lupus familiaris,Ovis aries.Zfy of Cervus elaphus was closely related to Bos taurus,Bison,Ovis aries,Pantholops hodgosonii compared with others.Zfx/Zfy genes of Cervus elaphus were cloned and sequenced for the first time,and this research provided a foundation for further studying the structure and biological functions of the proteins.

Key words: Cervus elaphus; Zfx/Zfy gene; clone; sequence analysis

CLC Number:

WEI Li-min, SUN Li-rong, ZHANG Yong-sheng, XI Ji-feng, LI Chao-cheng, WANG Xu-hai, WANG Xiang-zu, JIA Bin. Cloning and Sequence Analysis of Zfx/Zfy Gene in Cervus elaphus[J]. , 2016, 43(9): 2272-2278.

References

[1] Schneider-Gädicke A,Beer-Romero P,Brown L G,et al.Putative transcription activator with alternative isoforms encoded by human ZFX gene[J].Nature,1989,342(6250):708-711.
[2] Wu S,Lao X Y,Sun T T,et al.Knockdown of ZFX inhibits gastric cancer cell growth in vitro and in vivo via downregulating the ERK-MAPK pathway[J]. Cancer Lett,2013,337(2):293-300.
[3] Palmer C J,Galan-Caridad J M,Weisberg S P,et al.Zfx facilitates tumorigenesis caused by activation of the Hedgehog pathway[J].Cancer Res,2014,74(20):5914-5924.
[4] Kam J,Gresshoff P M,Shorter R.The Q-type C2H2 zinc finger subfamily of transcription factors in Triticum aestivum is predominantly expressed in roots and enriched with members containing an EAR repressor motif and responsive to drought stress[J].Plant Mol Biol,2008,67(3):305-322.
[5] Chandrasekharan S,Kumar S,Valley C M,et al.Proprietary science,open science and the role of patent disclosure:The case of zinc-finger proteins[J].Nat Biotechnol,2009,27(2):140-144.
[6] Mardon G,Page D C.The sex-determining region of the mouse Y chromosome encodes a protein with a highly acidic domain and 13 zinc fingers[J].Cell,1989,56(5):765-770.
[7] Han S H,Yang B C,Ko M S,et al.Length difference between equine ZFX and ZFY genes and its application for molecular sex determination[J].J Assist Reprod Genet,2010,27(12):725-728.
[8] Zhou Q,Bachtrog D.Ancestral chromatin configuration constrains chromatin evolution on differentiating sex chromosomes in drosophila[J]. PLoS Genet,2015,11(6):1-21.
[9] Skinner B M,Sargent C A,Churcher C,et al.The pig X and Y Chromosomes:Structure,sequence,and evolution[J].Genome Res,2016,26(1):130-139.
[10] Virta J,Markola J,Peippo J,et al.Sex determination of bovine embryo blastomeres by fluorogenic probes[J].Theriogenology,2002,57(9):2229-2236.
[11] Okuyama M W,Shimozuru M,Tsubota T.A genetic method for sex identification of raccoons (Procyon lotor) with using the ZFX and ZFY genes[J].J Vet Med Sci,2014,76(5):773-775.
[12] Salabi F,Nazari M,Cao W G.Cell culture,sex determination and single cell cloning of ovine transgenic satellite cells in vitro[J].J Biol Res (Thessalon),2014,21(1):21-22.
[13] Takabayashi S,Katoh H.Sex identification using the ZFX and ZFY genes in common marmosets (Callithrix jacchus)[J].Exp Anim,2011,60(4):417-420.
[14] Korstian J M,Hale A M,Bennett V J,et al.Advances in sex determination in bats and its utility in wind-wildlife studies[J].Mol Ecol Resour,2013,13(5):776-780.
[15] 金梅,傅忠扬,栾媛媛,等.辽宁新品系绒山羊ZFX、ZFY基因片段的进化研究与性别鉴定[J].畜牧兽医学报,2006,37(6):530-536.
[16] 熊勇,徐亚欧.藏系绵羊ZFX/ZFY基因片段进化分析[J].生物技术,2010,20(6):1-5.
[17] Iwase M,Satta Y,Hirai Y,et al.The amelogenin loci span an ancient pseudoautosomal boundary in diverse mammalian species[J].Pro Natl Acad Sci USA,2003,100(9):5258-5263.
[18] Gerrad,D T,Filatov D A.Positive and negative selection on mammalian Y chromosomes[J].Mol Bio and Evol,2005,22(6):1423-1432.
[19] Jegalian K,Page D C.A proposed path by which genes common to mammalian X and Y chromosomes evolve to become X inactivated[J]. Nature,1998,394(6695):776-780.
[20] Chibalina M V,Filatov D A.Plant Y chromosome degeneration is retarded by haploid purifying selection[J].Curr Biol,2011,21(17):1475-1479.
[21] Bergero R,Qiu S,Charlesworth D.Gene loss from a plant sex chromosome system[J].Curr Biol,2015,25(9):1234-1240.
[22] Koopman P,Gubbay J,Vivian N,et al.Male development of chromosomally female mice transgenic for Sry[J].Nature,1991,351(6322):117-121.
[23] Blackmon H,Demuth J P.Estimating tempo and mode of Y chromosome turnover:Explaining Y chromosome loss with the fragile Y hypothesis[J].Genetics,2014,197(2):561-572.
[24] 毛德才,徐亚欧,熊勇,等.牦牛与其他物种ZFX/ZFY基因片段间的进化关系[J].中国生物化学与分子生物学报,2007,23(6):450-456.

Cloning and Sequence Analysis of Zfx/Zfy Gene in Cervus elaphus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[2]	LYU Shuangfei, MA Xun, WANG Jing, KONG Cuilian, KOU Lijun, LIU Caixia, SHI Weidi, KANG Lichao, REN Huijie. Prokaryotic Expression and Immunoreactivity of PA-LF1 Fusion Protein of Bacillus anthracis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 674-683.
[3]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[4]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[5]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[6]	CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1135-1143.
[7]	HU Xiaobing, LIN Biaosheng, WANG Zhenwei, ZHENG Lijing. Isolation, Identification and Genomic Sequence Analysis of a Strain of Clostridium butyricum from Pigs Utilizing Lactic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 569-578.
[8]	KOU Meiling, MIAO Haisheng, LI Le, XIE Jiarui, LI Huachun, SONG Jianling. Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4401-4409.
[9]	WANG Miao, CHEN Xueyang, WANG Xingming, LIANG Xiongyan, YANG Yuying. Construction of Infectious Clones of Two ALV-K Recombinant Viruses and Virus Rescue [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3764-3770.
[10]	TAN Bin, WANG Chao, WANG Yan, ZHANG Shuqin. Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4012-4018.
[11]	WANG Zhiyan, SUN Yujie, XU Xin, HUANG Baicheng, TIAN Kegong. Isolation,Identification and Pathogenicity of Porcine Deltacoronavirus Strain HeN10 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2982-2989.
[12]	LI Nan, HE Yuwen, ZUO Yuanyuan, MENG Jinxin, XU Tiangang, WANG Jinglin. Analysis of Molecular Biological Characteristics of Structural Genes of Getah Virus (SZC30) Isolated from Midge [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1149-1160.
[13]	ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.
[14]	GUO Mengjiao, LI Mingtao, TAN Huihui, ZHANG Chengcheng, ZHANG Xiaorong, WU Yantao. Study on Cloning and Expression Characteristic of NLRC3 Gene in Rabbit [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3940-3949.
[15]	WANG Zhengfan, ZHU Lisai, LI Fei, XIANG Rui, LI Yiyun, YING Biyun, WANG Guiping, BAI Aiquan, JIA Aiqing. Pathogenicity and Whole Genome Sequence Analysis of Porcine Deltacoronavirus CH7328 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4143-4153.