猪繁殖与呼吸综合征病毒FS株Nsp9基因的克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2016.09.005

Abstract

Abstract:

In order to evaluate the function of Nsp9 gene,the target gene of porcine reproductive and respiratory syndrome virus (PRRSV) FS strain was amplificated and sequenced after being cloned into the pMD18-T vector.The physical and chemical properties,homology,hydrophilicity,surface probability plot,antigenic index,secondary structure and subcellular localization were predicted by various softwares.The results showed that the length of Nsp9 was 1 929 bp,its predicted molecular weight was 70.5 ku and pI was 7.78,and it was unstable protein.There were many antigen sites,and the flexibility and hydrophilicity of Nsp9 were ideal.The study showed that Nsp9 possessed potential antigenicity,and it fits for preparation of monoclonal antibodies.The results of subcellular location showed that it might exist in the cytoplasm.Nsp9 of FS strain shared 96.9% to 98.9% amino acid homology with other strains of the some hypotype.Some amino acid mutations were found between the parent strains and vaccine strains,and insertions and deletions could be found among the European strains and the American strains.Whether these insertions and deletions correlate with the virulence and polymerase needed further research.

Key words: porcine reproductive and respiratory syndrome virus; Nsp9 gene; clone; sequence analysis

CLC Number:

Q785

ZHAO Meng-meng, SONG Zhong-bao, FENG Song-lin, WANG Wen-jia, XING Xing, FENG Jia-ping, ZHANG Gui-hong. Cloning and Sequence Analysis of Nsp9 Gene of Porcine Reproductive and Respiratory Syndrome Virus FS Strain[J]. , 2016, 43(9): 2249-2256.

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Cloning and Sequence Analysis of Nsp9 Gene of Porcine Reproductive and Respiratory Syndrome Virus FS Strain

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