JSRV-Env重组质粒诱导转染TC-1、TC-1-Hyal2细胞后对TGF-α及VEGF因子表达的影响

doi:10.16431/j.cnki.1671-7236.2016.09.001

Abstract

Abstract:

JSRV-Env recombinant plasmid was transiently transfected with mouse lung epithelial cell (TC-1) and ovine Hyal-2 which could stablely express in TC-1 cell (TC-1-Hyal2) by the method of liposome,then we could explore the mRNA and protein expression of TGF-α and VEGF in order to analyze the correlation TGF-α and VEGF with ovine pulmonary adenomatosis (OPA) and what roles they played in the carcinogenesis process.Both cells were cultured in vitro,according to different plasmids,TC-1 and TC-1-Hyal2 cells were set pEGFP-C1-env transfected groups,pEGFP-C1 transfected and non-transfected groups.The expression of TGF-α and VEGF were detected through Real-time quantitative PCR and ELISA.The PCR results showed that compared with control groups (pEGFP-C1 and non-transfected cells groups),the mRNA expression of VEGF was significantly increased in two cell groups of both transfected with pEGFP-C1-env (P<0.05),while the mRNA expression of TGF-α was extremely significantly increased (P<0.01).The ELISA test results showed that compared with two control groups,the protein expression of VEGF and TGF-α were extremely significantly increased in TC-1-Hyal2 cell which transfected with pEGFP-C1-env (P<0.01),and in TC-1 cell which transfected with pEGFP-C1-env,the expression of VEGF protein was extremely significantly increased (P<0.01),the protein expression of TGF-α was significantly increased (P<0.05).There was no significant difference in mRNA and protein expression of two cytokines between pEGFP-C1-transfected and non-transfected cells groups (P>0.05).The results showed that the expression of TGF-α and VEGF both increased in two cell lines which transfected with JSRV-Env.The results suggested JSRV-Env might raise the expression of TGF-α and VEGF,futher we could assume that the TGF-α,VEGF expression changes had a correlation with OPA,and it could provide an important theoretical basis on OPA for gene targeting therapy.

Key words: JSRV-Env recombinant plasmid; TC-1 cells; TC-1-Hyal2 cells; TGF-α factor; VEGF factor

CLC Number:

Q786

LUO Shuang, SUN Li-hong, Siriguleng, YAO Hong-qiang. JSRV-Env Recombinant Plasmid Induced-transfection of TC-1 and TC-1-Hyal2 and Influence of Cytokine VEGF and TGF-α[J]. , 2016, 43(9): 2215-2222.

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JSRV-Env Recombinant Plasmid Induced-transfection of TC-1 and TC-1-Hyal2 and Influence of Cytokine VEGF and TGF-α

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