绵羊味觉受体第一家族基因外显子多态性分析

doi:10.16431/j.cnki.1671-7236.2016.08.022

Abstract

Abstract:

This study was aimed to investigate the distribution of genetic polymorphism of taste receptor family 1 member (T1R) gene between Ujimqin sheep and Hu sheep.DNA pools direct sequencing method and MALDI-TOFMS method were used to analyze genetic variation of T1R genes in 172 sheep of two Chinese sheep strains of Mongolian,and bioinformatics software predicted what impact polymorphic loci had to mRNA and protein secondary structure of T1R gene.The results showed that 9 SNPs were screened in T1R gene of two groups.Chi-square test for independence was taken to find the genotypes of the 5 SNPs which were significantly different between two sheep population(P<0.05),SNP2 located in TAS1R1 gene,SNP4,SNP7 and SNP8 located in TAS1R2 gene,SNP10 located in TAS1R3 gene.SNP2,SNP7 and SNP10 were silent mutations.SNP2 and SNP10 lead to corresponding gene mRNA secondary structure and the minimum free energy change,while the SNP7 only lead to the minimum free energy changes.SNP4 and SNP8 were missense mutations,the two missense mutations respectively led to asparagine(Asn) into serine (Ser),threonine (Thr) into methionine (Met),and according to online software forecast,the protein secondary structure of TAS1R2 gene all changed in mutations before and after.

Key words: sheep; taste receptor gene; polymorphism; boinformatics

CLC Number:

S813.3

XUAN Jun-li, MA Xiao-meng, YUAN Ze-hu, HU Shi-jin, WANG Hui-hua, WEI Cai-hong, ZHAO Fu-ping, ZHANG Li, DU Li-xin. Polymorphism Analysis of Taste Receptor Family 1 Member Gene Exons in Sheep[J]. , 2016, 43(8): 2081-2094.

References

[1] El-Sohemy A,Stewart L,Khataan N,et al.Nutrigenomics of taste-impact on food preferences and food production[J].Forum Nutr,2007,60:176-182.
[2] Garcia-Bailo B,Toguri C,Eny K M,et al.Genetic variation in taste and its influence on food selection[J].OMICS,2009,13(1):69-80.
[3] Roura1 E,Baldwin M W,Klasing K C,et al.禽类味觉系统研究进展[J].中国家禽,2012,34(12):44-47.
[4] 张根华,邓少平.味觉受体第一家族与味觉识别[J].生命的化学,2005,25(3):179-181.
[5] 王兴亚,庞广昌.哺乳动物味觉感受机制研究进展[J].四川动物,2014,33(5):785-791.
[6] Kuhn C,Meyerhof W.Oligomerization of sweet and bitter taste receptors[J].Methods Cell Biol,2013,117:229-242.
[7] Roper S D.Taste buds as peripheral chemosensory processors[J].Semin Cell Dev Biol,2013,24(1):71-79.
[8] Chaudhari N,Roper S D.The cell biology of taste[J].J Cell Biol,2010,190(3):285-296.
[9] Roper S D.Taste buds as peripheral chemosensory processors[J].Semin Cell Dev Biol,2013,24(1):71-79.
[10] Jiang P,Josue-Almqvist J,Jin X,et al.The bamboo-eating giant panda (Ailuropoda melanoleuca) has a sweet tooth:Behavioral and molecular responses to compounds that taste sweet to humans[J].PLoS One,2014,9(3):e93043.
[11] Bassoli A,Borgonovo G,Caremoli F,et al.The taste of D-and L-amino acids:In vitro binding assays with cloned human bitter (TAS2Rs) and sweet (TAS1R2/TAS1R3) receptors[J].Food Chem,2014,150:27-33.
[12] Tordoff M G,Alarcón L K,Valmeki S,et al.T1R3:A human calcium taste receptor[J].Sci Rep,2012,2:496.
[13] Margolskee R F,Dyer J,Kokrashvili Z,et al.T1R3 and gustducin in gut sense sugars to regulate expression of Na⁺-glucose cotransporter1[J].Proc Natl Acad Sci USA,2007,104(38):15075-15080.
[14] Moran A W,Al-Rammahi M,Zhang C,et al.Sweet taste receptor expression in ruminant intestine and itsactivation by artificial sweeteners to regulate glucose absorption[J].J Dairy Sci,2014,97(8):4955-4972.
[15] Damak S,Rong M,Yasumatsu K,et al.Detection of sweet andumami taste in the absence of taste receptor T1R3[J].Science,2003,301(5634):850-853.
[16] Zhao G Q,Zhang Y,Hoon M A,et al.The receptors for mammalian sweet and umami taste[J].Cell,2003,115(3):255-266.
[17] Shi P,Zhang J.Contrasting modes of evolution between vertebrate sweet/umami receptor genes and bitter receptor genes[J].Mol Biol Evol,2006,23(2):292-300.
[18] Hashiguchi Y,Furuta Y,Kawahara R,et al.Diversification and adaptive evolution of putative sweet taste receptors in threespine stickleback[J].Gene,2007,396(1):170-179.
[19] Zhao H,Zhou Y,Pinto C M,et al.Evolution of the sweet taste receptor gene TAS1R2 in bats[J].Mol Biol Evol,2010,27(11):2642-2650.
[20] Zhao H,Yang J R,Xu H,et al.Pseudogenization of the umami taste receptor gene TAS1R1 in the giantpanda coincided with its dietary switch to bamboo[J].Mol Biol Evol,2010,27(12):2669-2673.
[21] Niimura Y,Nei M.Evolutionary dynamics of olfactory and other chemosensory receptor genes in vertebrates[J].J Hum Genet,2006,51(6):505-517.
[22] Li X,Li W,Wang H,et al.Pseudogenization of a sweet-receptor gene accounts for cats' indifference toward sugar[J]. PLoS Genet,2005,1(1):27-35.
[23] 杨嵩,沈峻宇,陈定超,等.家兔TAS1R2基因与生长发育性状的关联性分析[J].四川畜牧兽医,2015,2015(3):29-31.
[24] 李俊,张勇,杨红,等.从江香猪S1M1基因SNPs筛查及生物信息学分析[J].中国畜牧兽医,2015,42(9):2224-2232.
[25] 曲娟娟,于敏,董蕾,等.SNP在动植物遗传育种中的应用[J].东北农业大学学报,2011,42(12):9-13.
[26] 李树珍,万慧荣,杨光.DNA池结合DHPLC和直接测序技术在江豚SNPs检测中的应用[J].兽类学报,2009,29(2):185-190.
[27] 宋桃伟,蔡惠芬,罗卫星.两个贵州山羊品种GHSR和GHRL基因遗传变异及其与生长性状的关联[J].中国农业科学,2015,48(1):140-153.
[28] Liu B,Ha M,Meng X Y,et al.Molecular mechanism of species-dependent sweet taste toward artificial sweeteners[J].J Neurosci,2011,31(30):11070-11076.
[29] Liu Y,Chu A,Chakroun I,et al.Cooperation between myogenic regulatory factors and SIX family transcription factors is important for myoblast differentiation[J].Nucleic Acids Res,2010,38(20):6857-6871.
[30] Omar R L,Arturo P,Erika M L,et al.Sweet taste receptor TAS1R2 polymorphism(Val191Val) is associated with a higher carbohydrate intake and hypertriglyceridemia among the population of West Mexico[J].Nutrients,2016,8(2):E101.
[31] Andre G D,Karen M E,Moira C,et al.Variation in the TAS1R2 gene,sweet taste perception and intake of sugars[J].Journal of Nutrigenetics & Nutrigenomics,2015,8(2):81-90.
[32] Zhang C L,Yuan J,Wang Q,et al.The association of bovine T1R family of receptors polymorphisms with cattle growth traits[J].Res Vet Sci,2012,93(2):783-787.

Polymorphism Analysis of Taste Receptor Family 1 Member Gene Exons in Sheep

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