救必应水提取物对产ESBLs细菌的抑菌机理研究

doi:10.16431/j.cnki.1671-7236.2016.06.021

Abstract

Abstract: The study was undertaken to study the inhibitory effect and mechanism of the water extracts of Holly Bark.Minimal inhibitory concentration (MIC) was tested through twice micro-dilution method and minimal bactericidal concentration (MBC) was tested by agar plate dilution counting method (SDA)of the water extracts of Holly Bark on producing extended spectrum β-lactamases (ESBLs) bacteria,then the bacteriostatic mechanism of the water extracts of Holly Bark through bacteria curve was tested,the cell ultrastrueture was observed,permeabilty changes of cell wall and membrane,nucleic acid synthesis inhibitory experiment were researched.The results showed that the water extracts of Holly Bark could affect bacteria curve compared with the blank control,destroy the cells seriously,permeability of both cell membrane and wall increased,extravasation of cytoplasm appeared,the content of AKP and soluble protein in the bacterial liquid increased,E.coli DNA fluorescence intensity weakened obviously.Research showed that the bacteriostatic mechanism of the water extracts of Holly Bark was based on its roles about changes on the cell wall and membrance permeability,inhibitting nucleic synthesis of bacterial.

Key words: Holly Bark; producing extended spectrum β-lactamases bacteria; minimal inhibitory concentration; minimal bactericidal concentration; antibactericidal mechanism

CLC Number:

S855.1

SONG Jian-wu, WU Yong-ji, SUN Yan-jie, LIU Zeng-yuan, ZHAO Yu-chuan, HU Mei, XIA Juan, Huang Kai, SI Hong-bin. Study on the Bacteriostatic Mechanism of the Water Extracts of Holly Bark on ESBLs-producing Bacterial[J]. , 2016, 43(6): 1536-1543.

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Study on the Bacteriostatic Mechanism of the Water Extracts of Holly Bark on ESBLs-producing Bacterial

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