3种动物专用抗菌药在鸡肠道沙门菌的流行病学临界值的建立

doi:10.16431/j.cnki.1671-7236.2020.09.026

Abstract

Abstract: In order to establish epidemiological cut-off values (ECOFFs) of Salmonella for three animal-specific antibiotics (florfenicol,apramycin and danofloxacin) in Guangdong province.A total of 166 strains of Salmonella isolates from chicken intestinal tract from veterinary clinics and farms in Guangdong province in 2017 were recruited in this study,the minimum inhibitory concentrations (MIC) of florfenicol,apramycin and danofloxacin were determined in triplicate for each bacterial strain using the agar dilution method on Mueller-Hinton agar plates according to the CLSI reference method (CLSI-M100-S26).ECOFFs were calculated for the MIC data sets by application of nonlinear regression analysis,NRI(normalized resistance interpretation) and ECOFFinder software.MIC distribution of florfenicol,apramycin and danofloxacin against Salmonella from 2 to >512,4 to >512 and 0.015 to 64 μg/mL,respectively.MIC₅₀ and MIC₉₀ for apramycin,florfenicol and danofloxacin were 256 and >512,16 and >512,0.5 and 16 μg/mL,respectively.MIC distribution of apramycin and florfenicol presented obvious unimodal shape,while that of danofloxacin was a discontinuous multi-peak.The ECOFFs of florfenicol,apramycin and danofloxacin were recommended to be 16,16 and 0.125 μg/mL,respectively.In present study,the ECOFFs of Salmonella from chicken intestinal tract for apramycin,florfenicol and danofloxacin were determined,which provided scientific basis for resistance surveillance.

Key words: veterinary drugs; minimal inhibitory concentration (MIC); Salmonella; epidemiological cut-off values (ECOFFs)

CLC Number:

S852.61⁺2

WU Sili, FU Jiali, ZHU Jiahang, ZHANG Yan, DING Xiaomin, JIANG Hongxia. Epidemiological Cut-off Values (ECOFFs) of Salmonella from Chicken Intestinal Tract for Three Animal-specific Antibiotics[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2917-2925.

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Epidemiological Cut-off Values (ECOFFs) of Salmonella from Chicken Intestinal Tract for Three Animal-specific Antibiotics

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