云南大额牛瘤胃微生物多样性分析

doi:10.16431/j.cnki.1671-7236.2016.05.006

Abstract

Abstract: In order to study the rumen microbial diversity in Yunnan gayals (Bos frontalis), we analyzed the diversity of microbes using largely assembled sequence data obtained by metagenomic sequencing DNA isolated from rumen samples of eight adult galyals. The genome primary sequence (total metagenome size was >3 Gb) was assembled with short reads.Based on the gayal rumen microbial metagenomic sequencing, we received a total of 13 546 196 EGTs. Because of the relatively large amount, 10 387 566 EGTs (76.68% of all EGTs ) were randomly selected to analyze microbial communities by BLAST search of the NCBI-NR database. Microbial species annotations analysis revealed that microbial communities from gayals rumen were mainly composed of the bacterial domain, eukarya domain, archaea domain and unknown microbial composition. The majority microbes mainly belonged to ten phylums, and 94% EGTs belonged to Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria, of which the highest abundance phylums were Bacteroidete and Firmicutes, accounting for 48% and 42%, respectively. In addition, the main cellulolytic bacteria in rumen of gayals were Ruminococcus flavefacie, Butyrivibrio fibrisolvens, Ruminococcus albus and Fibrobacter succinogenes. They accounted for 1.60%, 1.20%, 0.86% and 0.52% of the total bacteria, respectively. These results further indicated that EGTs data could be used to analyze and explain environmental microbial community structure and function. There was great significance to study the composition of microorganisms in special environments.

Key words: Yunnan gayals (Bos frontalis); metagenomics sequences; EGTs; microbial communities

CLC Number:

S823

ZHOU Xiong-yan, WANG Song-ming, GU Zhao-bing, WU Xi-chuan, LENG Jing, MAO Hua-ming, LI Qing, FENG Li, YANG Shu-li. Diversity Analysis of Rumen Microbes in Yunnan Gayals (Bos frontalis)[J]. , 2016, 43(5): 1162-1168.

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Diversity Analysis of Rumen Microbes in Yunnan Gayals (Bos frontalis)

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