维生素E通过间隙连接蛋白Cx43对牛颗粒细胞凋亡及增殖的影响

doi:10.16431/j.cnki.1671-7236.2016.03.008

Abstract

Abstract: The aim of this study was to determine the effect of vitamin E on Cx43,the mechanism and function of vitamin E on bovine granulosa cells apoptosis and proliferation.In this study,granulosa cells were isolated from bovine ovary and cultivated in vitro by adding different concentration of vitamin E (0,25,50,100,200 and 500 μmol/L) for 24 h.After cultured,apoptotic cells were detected by FCM,mRNA expression levels of BCL2/BAX、P53 and Cx43 genes were determined by Real-time PCR and cell proliferation was detected by CCK8.The results showed that compared to control group,100 μmol/L vitamin E could significantly inhibit the apoptosis of granulosa cells (P<0.05).Real-time PCR detection results showed that vitamin E significantly changed the mRNA expression levels of BCL2/BAX,P53,Cx43 genes (P<0.05).Vitamin E could significantly improve granulosa cells proliferation when granulosa cells were treated for 24 and 36 h (P<0.05).The results provided a theoretical basis on further analysis for studing the influence mechanism of vitamin E on oocytes development and maturity,and improvement of female animal reproduction by influencing granulosa cells proliferation and apoptosis.

Key words: vitamin E; granulosa cell; Connexin 43; proliferation; apoptosis

CLC Number:

Q566

REN Shu-qiang, WANG Jia-wei, CHEN Hong-yan, XU Ming-qiang, JIANG Hao, GAO Yan, ZHOU Jian-zhong, ZENG Fan-ming, LI Cheng-sheng, ZHANG Jia-bao, CHEN Cheng-zhen. Effect of Vitamin E on Bovine Granulosa Cells Apoptosis and Proliferation through Cx43[J]. , 2016, 43(3): 615-621.

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Effect of Vitamin E on Bovine Granulosa Cells Apoptosis and Proliferation through Cx43

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