新吉细毛羊角蛋白的EST筛选及表达研究

doi:10.16431/j.cnki.1671-7236.2015.11.009

Abstract

Abstract: The experience was conducted to screen the genes associated with hair follicle development from the expressed sequence tags (ESTs) derived from the skin tissues of Xinji fine wool sheep and explore the association between their expression patterns and the fineness of wool.Using Trinity and Blat,we screened out the candidate genes by assembly and homologous annotation of ESTs,and further studied their expression patterns through detecting the correlation value 2^-△△Ct by Real-time PCR method.As a result,four genes (KRT27,KAP3.2,KAP11.1 and KAP8.1) were carried out to be responsible for hair follicle development,and 4 keratin genes were extremely significant more highly expressed in skin tissues of super fine wool sheep than in fine wool sheep (P<0.01);The expression level of 4 keratin were as follow:KRT27 >KAP3.2 >KAP11.1 >KAP8.1.The study provided certain data for the research on transcript level of fine wool sheep keratin gene.

Key words: EST sequence; keratin gene; RT-PCR; significant expression

CLC Number:

S826

LIU Chun-jie, FU Xue-feng, TIAN Ke-chuan, HUANG Xi-xia, XU Xin-ming, ZHAN Zhen-hong, YU Li-juan. Screening and Expression of Keratin EST in Xinji Fine Wool Sheep[J]. , 2015, 42(11): 2873-2879.

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Screening and Expression of Keratin EST in Xinji Fine Wool Sheep

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