猪源大肠杆菌O157:H7鞭毛蛋白抗原表位分析及验证

doi:10.16431/j.cnki.1671-7236.2015.11.006

Abstract

Abstract: To understand the pathogenicity of Escherichia coli O157:H7,the secondary structures,hydrophilicity plot,antigenic index,flexibility plot and surface probability plot of the flagellin FliC of Escherichia coli O157:H7 were analyzed by bioinformatics.The α-helix,β-sheet,turn and coil regions of flagellin FliC were analyzed by the methods of Garnier-Robson and Chou-Fasman of DNAStar software,hydrophilicity plot was used the method of Kyte-Doolittle,the flexibility plot was based on the Karplus-Schulz method,surface probability plot and antigenic index were analyzed by the methods of Emini and Jameson-Wolf,respectively.The results showed that 63 to 74,236 to 247,338 to 349,460 to 471 and 542 to 553 amino acid sites of flagellin FliC might be B cell antigen epitopes.338 to 349 and 460 to 471 peptides were synthesized by chemical synthesis,and then subcutaneously immunized BALB/c mice three times.Antibody response was evaluated by ELISA.The result showed that 338 to 349 and 460 to 471 peptides could induce BALB/c mice to produce high titer antibody.

Key words: Escherichia coli O157:H7; flagellin; B cell antigen epitopes; immunity

CLC Number:

S852.4

LIN Min-ling, LI Jun, PAN Yan, CHEN Ze-xiang, YANG Wei, HU Ting-jun. Antigen Epitopes Analysis and Verification of Escherichia coli O157:H7 Flagellin[J]. , 2015, 42(11): 2856-2861.

References

[1] Tarr P I,Gordon C A,Chandler W L.Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome[J].Lancet,2005,365(9464):1073-1086.
[2] Fukushima H,Hashizume T,Morita Y,et al.Clinical experiences in Sakai city hospital during the massive outbreak of enterohemorrhagic Escherichia coli O157 infections in Sakai city,1996[J].Pediatr Int,1999,41(2):213-217.
[3] 李军,谢宇舟,冯世文,等.猪源性大肠杆菌O157:H7耐药表型和消毒剂抗性调查[J].中国畜牧兽医,2012,39(2):203-206.
[4] Mahajan A,Currie C G,Mackie S,et al.An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157:H7 with bovine intestinal epithelium[J].Cell Microbiol,2009,11(1):121-137.
[5] Macnab R M.How bacteria assemble flagella[J].Annual Review Microbiology,2003,57:77-100.
[6] Haiko J,Westerlund-Wikstr m B.The role of the bacterial flagellum in adhesion and virulence[J].Biology (Basel),2013,2(4):1242-1267.
[7] Wang L,Rothemund D,Curd H,et al.Species-wide variation in the Escherichia coli flagellin (H-antigen) gene[J].Bacteriol,2003,185(9):2936-2943.
[8] Winstanley C,Morgan J A.The bacterial flagellin gene as a biomarker for detection,population genetics and epidemiological analysis[J].Microbiology,1997,143(10):3071-3084.
[9] 张雪寒,何孔旺,赵攀登,等.肠出血大肠杆菌O157:H7鞭毛可变区基因片段的克隆、表达与免疫原性[J].江苏农业学报,2011,27(5):1021-1025.
[10] Kwang J,Wilson R,Yang S,et al.Mapping of the H7-serospecific domain of Escherichia coli flagellin[J].Clin Diagn Lab Immunol,1996,3(5):523-526.
[11] 谢宇舟,李军,禤雄标,等.广西畜禽大肠杆菌O157:H7流行病学调查[J].中国人兽共患病学报,2011,27(12):1151-1155.
[12] Schwan W R.Flagella allow uropathogenic Escherichia coli ascension into murine kidneys[J].Int J Med Microbiol,2008,298(5-6):441-447.
[13] Ramos H C,Rumbo M,Sirard J C.Bacterial flagellins:Mediators of pathogenicity and host immune responses in mucosa[J].Trends Microbiol,2004,12(11):509-517.
[14] 段强德.F18⁺大肠杆菌鞭毛与其致病相关性的研究[D].扬州:扬州大学,2012.
[15] 谢宇舟,密克,李军,等.猪源大肠杆菌O157:H7与O157的差异蛋白质组学分析[J].基因组学与应用生物学,2012,31(6):531-537.

Antigen Epitopes Analysis and Verification of Escherichia coli O157:H7 Flagellin

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XIE Hualiang, LEI Xiaowen, LI Jianjun, LIU Zhenni, ZHONG Yunping, TAN Donghai, ZHANG Qiang. Effects of Earthworm Hydrolysate on Laying Performance,Serum Biochemical Indices,Antioxidant Indices and Immune-related Indices of Laying Hens Aged 105 to 175 Days [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1899-1906.
[2]	JIANG Haijun, WANG Dan, GUO Yu, WEI Li, HOU Lei, WANG Jing, ZHU Shanshan, LIU Jue. Fusion Expression and Immunogenicity of Recombinant Cap-flagellin Protein of Porcine Circovirus Type 3 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2044-2052.
[3]	LIU Ying, TIAN Xu, FENG Xiaomeng, LYU Xiaoping, GAO Xueli, ZHENG Shimin, LIU Chaonan. Protective Effect of Astragalus Polysaccharide on Intestinal Mucosal Injury in Chicks with Enteritis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 76-85.
[4]	WANG Yue, ZHENG Yunxi, XU Songsong, XIANG Guangming, LI Hua, WANG Nan, FENG Zheng, LI Kui, MU Yulian. Regulation of Wip1 Gene on Animal Reproduction and Immunity [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3500-3507.
[5]	JIA Xinyue, MA Jing, ZHANG Yanyan, MA Xun, WANG Zhengrong, BO Xinwen. Research Progress on Oral Vaccine of Animal Parasites [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3569-3580.
[6]	SHI Qian, XU Xinfeng, LI Xuewen, XIAO Yuanyuan, BAI Meihua, ZHANG Fuchun. Research Progress on the Mechanism of Polysaccharide as Adjuvant of Veterinary Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1895-1907.
[7]	ZHU Cui, HUANG Kaiyong, LI Lina, WU Aiji, WU Peng, FU Fangmiao, LUO Yuanhua, ZHANG Huihua. Effects of Berberine on Organ Index, Antioxidant Capacity and Intestinal Immunity in Yellow-feathered Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 932-941.
[8]	ZHANG Qiang, LIU Zhenni, LIAO Qiang, LEI Xiaowen, KONG Zhiwei, XIE Hualiang, TAN Donghai, LI Jianjun, ZHONG Yunping. Effects of Fermented Andrographis Powder on Production Performance, Serum Biochemical Indexes, Immune Related Indexes, Antioxidant Indexes and Fatty Acid Contents of Muscle in Muscovy Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4614-4624.
[9]	LI Xiangbao, XU Xiaohui, AN Xiuxiu, WEI Xueliang, XU Yuwei, WANG Yuanyuan, CHEN Shi. Effects of Rumen-protected Choline and Organo-chromium on Immunity and Antioxidant Properties of Perinatal Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4646-4653.
[10]	MA Xingshu. Advances on Trained Immunity of Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4756-4775.
[11]	MA Yujing, YANG Ling, HE Rongxiang, HE Jianhua. Physiological Functions of Fucoidan and Its Application in Animal Production [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2404-2412.
[12]	AN Tao, ZHANG Hailiang, WANG Yachun. Analysis on Disease Resistance of Immunity⁺ Bulls' Daughters [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1791-1799.
[13]	LUO Zhicheng, LIU Zhe, QUAN Jinqiang, KANG Yujun, QIN Yong. Effects of Additive Amount of Antimicrobial Peptides on Biochemical Indices of Hybrid Sturgeon [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1452-1458.
[14]	YANG Shuai, HU Xiangwei, ZHOU Di, ZHANG Xinxin, YANG Liguo. Study on the Growth Promoting Effect of Crossbred Buffalo Calves Immunized with DNA Vaccine of Co-expression of Somatostatin and Cortistatin [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4041-4050.
[15]	LI Hongmin, DONG Li, HU Zhenzhen, MAO Junzhou, ZHANG Yongsheng, LIU Jun, YU Lihuai. Effects of Attapulgite Nano Zinc Oxide on Antioxidant Function and Immune Function in Spleen of Weaned Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3484-3492.