猪嵴病毒VP0基因的克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2015.09.013

Abstract

Abstract: In this study, specific primers were designed according to VP0 gene of porcine kobuvirus (PKV), and the full-length coding region of VP0 gene was amplified by RT-PCR method and sequenced, then bioinformatics analysis were conducted to investigate the structure and function of the porcine kubovirus VP0 gene.The results showed that the porcine kobuvirus VP0 gene was 1 098 bp in length, coding an open reading frame (ORF) with 366 amino acids.The isoelectric point, molecular weight and instability index of porcine kubovirus VP0 were 6.71, 38.489 ku, 34.65, respectively.The maximum and minimum hydrophobicity were 2.622 and —2.122, respectively.Compared with the porcine kubovirus VP0 genes published previously in GenBank, the sequenced gene shared the highest homology with HNXX-4 strain, which was 89.1%;And shared the lowest homology with S-1-HUN strain, which was 81.1%.The porcine kubovirus VP0 gene shared two different phylogenetic genotype branches, and the Chinese porcine kubovirus isolates distributed in the two phylogenetic genotype branches.

Key words: porcine kobuvirus; VP0 gene; clone; sequence analysis

CLC Number:

S852.65

CHEN Ru-jing, XIU Jin-sheng, CHEN Xiao-luo, WU Xue-min, CHE Yong-liang, WANG Chen-yan, YAN Shan, WANG Long-bai, ZHOU Lun-jiang. Cloning and Sequence Analysis of the VP0 Gene of Porcine Kobuvirus[J]. , 2015, 42(9): 2303-2307.

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Cloning and Sequence Analysis of the VP0 Gene of Porcine Kobuvirus

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