猪细环病毒1a型ORF1基因的克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2015.07.007

Abstract

Abstract: In order to know the ORF1 gene of the Fujian isolates of porcine Torque teno sus virus (PTTSuV) type 1a,the study amplified the ORF1 gene of PTTSuV type 1a from the DNA isolated in the feces with diarrhea in Fujian province.The target PCR fragments were cloned and sequenced,and spliced with SeqMan software.The results showed that the cloned ORF1 gene of PTTSuV type 1a was 1 947 bp in length,coding an open reading frame (ORF) with 648 amino acids,its theoretical isoelectric point was 10.06.The nucleotide sequence and the amino acid sequence deduced from the gene were analyzed by the bioinformatics software.Compared with the PTTSuV type 1a ORF1 gene from GenBank,the sequenced gene shared the highest homology with TTV1 Bj2-1 strain (GenBank accession number:HM633243) at 99.7%.The phylogenetic tree was constructed with Mega 6.06,the PTTSuV type 1a could be divided into 2 different phylogenetic clusters (clusterⅠ and cluster Ⅱ),the cluster Ⅰ also had two distinguish branch (Ⅰa and Ⅰb).The results of this study could enrich the genomic characterization of PTTSuV type 1a in Fujian.

Key words: porcine Torque teno sus virus type 1a; ORF1 gene; clone; sequence analysis

CLC Number:

S852.65

CHEN Peng-qiang, HU Chong-wei, LIN Qun-qun, CHEN Qiu-yong, XIU Jin-sheng. Cloning and Sequence Analysis of ORF1 Gene of Porcine Torque Teno sus Virus Type 1a[J]. , 2015, 42(7): 1668-1673.

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Cloning and Sequence Analysis of ORF1 Gene of Porcine Torque Teno sus Virus Type 1a

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