大白猪肿瘤坏死因子α基因启动子区多态性及其对mRNA表达水平的影响

doi:10.16431/j.cnki.1671-7236.2015.06.029

Abstract

Abstract: Tumor necrosis factor α (TNF-α) gene plays an important role in innate immunity and inflammatory responses,this study aimed at analyzing the relationship between the polymorphism of TNF-α gene promoter and gene expression in Large White pigs,and further providing a scientific basis for analyzing the genetic effect of TNF-α gene promoter C-791T mutation.This study detected the polymorphism at C-791T site of TNF-α gene promoter in 201 heads of Large White pigs by PCR-SSCP method.Meanwhile,the expression of TNF-α gene in 11 tissues was assayed by Real-time PCR and analyzed the differential expression among individuals with different genotypes at C-791T site.The results showed that there existed one mutation (C-791T) in TNF-α gene promoter and three genotypes were detected,which were CC, CT and TT.The mRNA expressions of TNF-α were higher in spleen,lung,thymus and lymph of weaned piglets.TNF-α gene expression levels of TT and CT genotypes individual were generally higher than CC genotype individual in 11 tissues,and the mRNA expression in TT and CT individuals were significant higher than that in CC individuals in the duodenum and jejunum tissue (P <0.05).Therefore,the C-791T mutation of TNF-α gene promoter indeed had a significant influence on the mRNA expression level of TNF-α gene,which indicated that it was necessary for us to carry out an in depth study of TNF-α gene promoter C-791T polymorphism as genetic markers of enhancing the resistance to intestinal pathogenic bacteria.

Key words: pig; TNF-α gene; polymorphism; differential expression

CLC Number:

S813.3

SUN Li, XIA Ri-wei, LIU Ying, SUN Shou-yong, BAO Wen-bin, WU Sheng-long. Polymorphism of TNF-α Gene Promoter and its Effect on mRNA Expression in Large White Pigs[J]. , 2015, 42(6): 1518-1523.

References

[1] Bradley J R.TNF-mediated inflammatory disease[J].The Journal of Pathology,2008,214(2):149-160.

[2] Menon R,Merialdi M,Betran A P,et al.Analysis of association between maternal tumor necrosis factor-α promoter polymorphism (-308),tumor necrosis factor concentration,and preterm birth[J].American Journal of Obstetrics and Gynecology,2006,195(5):1240-1248.

[3] Namian A M,Shahbaz S,Salmanpoor R,et al.Association of interferon-gamma and tumor necrosis factor alpha polymorphisms with susceptibility to vitiligo in Iranian patients[J].Archives of Dermatological Research,2009,301(1):21-25.

[4] Lanas A,Garcia-Gonzalez M A,Santolaria S,et al.TNF and LTA gene polymorphisms reveal different risk in gastric and duodenal ulcer patients[J].Genes and Immunity,2001,2(8):415-421.

[5] Hohler T,Kruger A,Gerken G,et al.Tumor necrosis factor alpha promoter polymorphism at position-238 is associated with chronic active hepatitis C infection[J].Journal of Medical Virology,1998,54(3):173-177.

[6] 魏茂提,韩燚,何丽,等.TNF-α启动子区基因多态性与SARS易感性及症状关系研究[J].武警医学院学报,2009,18(3):169-174.

[7] Szydlowski M,Buszka A,Mackowski M,et al.Polymorphism of genes encoding cytokines IL-6 and TNF is associated with pig fatness[J].Livestock Science,2011,136(2-3):150-156.

[8] Schmitz H,Fromm M,Bentzel C J,et al.Tumor necrosis factor-alpha (TNF alpha) regulates the epithelial barrier in the human intestinal cell line HT-29/B6[J].Journal of Cell Science,1999 112(1):137-146.

[9] Raz E.Organ-specific regulation of innate immunity[J].Nature Immunology,2007,8(1):3-4.

[10] 陈逢,刘玉兰,李权,等.TLR4信号通路关键基因在断奶仔猪不同组织中的mRNA表达[J].中国畜牧杂志,2013,49(17):53-57.

[11] Clay H,Volkman H E,Ramakrishnan L.Tumor necrosis factor signaling mediates resistance to mycobacteria by inhibiting bacterial growth and macrophage death[J].Immunity,2008,29(2):283-294.

[12] 施开创,杨汉春,郭鑫,等.猪繁殖与呼吸综合征病毒和猪圆环病毒2型共感染对外周血单个核细胞促炎细胞因子mRNA表达的影响[J].畜牧兽医学报,2009,40(3):363-370.

[13] 郑维豪,杜红丽,林志强,等.猪肺泡巨噬细胞感染 PRRSV 进程中差异表达基因及基因功能富集分析[J].基因组学与应用生物学,2010,29(6):1039-1046.

[14] 冯丽丽,张丽萍,张改平,等.脂多糖对猪肺泡巨噬细胞分泌IL-1β及TNF-α的影响[J].河南农业科学,2009,3:106-109.

[15] 王冬梅,冯亚琼,崔春晓,等.脂多糖对猪繁殖与呼吸综合征病毒诱导的抗病毒免疫反应的影响[J].中国畜牧兽医,2014,41(8):206-210.

[16] Harari Y,Weisbrodt N W,Moody F G.Ileal mucosal response to bacterial toxin challenge[J].The Journal of Trauma and Acute Care Surgery,2000,49(2):306-313.

[17] Kiyono H,Kweon M N,Hiroi T,et al.The mucosal immune system:From specialized immune defense to inflammation and allergy[J].Acta Odontologica Scandinavica,2001,59(3):145-153.

[18] McKay D M,Baird A W.Cytokine regulation of epithelial permeability and ion transport [J]. Gut,1999,44(2):283-289.

[19] Günther C,Buchen B,He G W,et al.Caspase-8 controls the gut response to microbial challenges by TNF-α-dependent and independent pathways[J].Gut,2015,64(4):601-610.

[20] Fischer A,Gluth M,Pape U F,et al.Adalimumab prevents barrier dysfunction and antagonizes distinct effects of TNF-α on tight junction proteins and signaling pathways in intestinal epithelial cells[J] American Journal of Physiology Gastrointestinal and Liver Physiology,2013,304(11):970-979.

Polymorphism of TNF-α Gene Promoter and its Effect on mRNA Expression in Large White Pigs

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[3]	CHEN Cui, GONG Lei, XU Zhe, WANG Xiaobo. Study on the Inhibition of Hydrogen Peroxide-induced Cellular Aging by AMPK [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2255-2264.
[4]	XU Yuanyuan, ZHENG Haiying, YANG Chunyan, DENG Tingxian, HUANG Chenqian, FENG Chao, LU Xingrong, DUAN Anqin, MO Xia, MA Xiaoya, SHANG Jianghua. Effects of Inhibition of BMP/Smad Signaling Pathway on the Growth and Steroid Hormone Synthesis of Buffalo Ovarian Granulosa Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2354-2362.
[5]	ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.
[6]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2380-2387.
[7]	SUN Shouhu, CHEN Zhifei, GUO Zhixuan, YU Ting, WANG Xiuwu, LI Songbei, WEI Wenkang, HE Dongsheng. Study on Codon Usage Bias of N gene of Three Porcine Coronaviruses and Their Host Adaptive Mechanism [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2403-2413.
[8]	LI Yongbin, YANG Huilin, SONG Zihao, ZHANG Yu, YANG Jin, MENG Jia, CUI Xiaozhen, LYU Xiaoling, ZHENG Mingxue, BAI Rui. Cloning and Bioinformatics Analysis of MIF Gene in Eimeria tenella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2450-2459.
[9]	SHI Qiuying, DUAN Baomin, LI Ying, NIU Dengyun, GENG Chenyang, ZHOU Jiawei, WANG Rui, FENG Jingjing. Analysis of Genetic Variation of S1 Gene of Porcine Epidemic Diarrhea Virus in Some Areas of China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2468-2478.
[10]	OU Yunwen, PAN Qin, WANG Yang, DAI Junfei, REN Shaoke, ZHANG Yang, ZHANG Jie. Truncated Expression of ORF2 Gene of Porcine Parvovirus Type 6 and Preparation of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2487-2495.
[11]	CHEN Jianshi, ZHANG Ning, PU Fangxiong, MIAO Jianjia, ZENG Nan, LI Bingxue. Prediction of Bacillus anthracis Carrying Prophages and Analysis of Drug Resistance and Virulence [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2573-2584.
[12]	ZHANG Dianqi, MA Xinhao, YANG Zhimei, MEI Chugang, ZAN Linsen. Bioinformatics Analysis of PAI1 Gene and Its Effect on the Proliferation and Differentiation of Intramuscular Adipocytes in Qinchuan Beef Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1729-1741.
[13]	PIAN Huifang, DU Xubin, LI Yan, ZHANG Yuchen, LIU Fei, YU Debing. Sequencing Analysis of the Full-length Transcriptome of Granulosa Cells of Small Yellow Follicle in Hy-Line Variety Brown [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1754-1763.
[14]	WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.
[15]	CHEN Meiting, ZHENG Congsen, LIANG Zexian, LIN Qiaoer, CHANG Chuanzhe, ZHOU Jun, FENG Jun, LI Linlin, QIN Limei. Cloning and Bioinformatics Analysis of env Gene of Avian Leukosis Virus Subgroup J Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1785-1795.