波尔山羊线粒体融合蛋白2基因的克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2015.05.006

Abstract

Abstract: Mitofusin-2 (MFN2), an outer transmembrane protein on the mitochondrion, which was a signaling and therapeutic target molecule, played a critical role in cell proliferation, differentiation, apoptosis and many diseases.For further researching the molecular mechanism of MFN2 gene in inflammatory disease, the MFN2 gene from Boer goat was cloned by RT-PCR, and its sequence and coding protein structure were analyzed.The results showed that the full length of MFN2 gene was 2 441 bp and its coding protein was composed of 705 amino acids.It was low homology between species with conservative protein structure, Fzo-mitofusin structural domain and DLP-2 structural domain, respectively.The MFN2 had a potential transmembrane domain, and was consist of 576 to 595 amino acid without signal peptide.It was the first time to clone MFN2 gene from Boer goat, which provided basic data for exploring the molecular mechanism of the relationship between MFN2 gene from Boer goat and inflammation.

Key words: Boer goat; mitofusin-2 (MFN2); clone; sequence analysis

CLC Number:

YE Jin-hui, ZHANG Kai-zhao, YU Meng, WANG Qing-nan, LIU Jian-xin, TAO Pan, NING Zhang-yong. Cloning and Sequence Analysis of MFN2 Gene from Boer Goat[J]. , 2015, 42(5): 1076-1081.

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Cloning and Sequence Analysis of MFN2 Gene from Boer Goat

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