猪BPI蛋白重组载体的构建及真核表达

doi:10.16431/j.cnki.1671-7236.2015.04.011

Abstract

Abstract: In order to construction porcine bactericidal/permeability-increasing protein (BPI) eukaryotic expression vector and obtain porcine BPI protein recombinant vector. In this study, BPI coding sequence was cloned into vector pUC57 according to GenScript's CloneEZ^® PCR Cloning Kit. After correct identification with digestion and sequencing, the recombinant plasmid pUC57-BPI was transfected to 293-6E cells, then the expression of the recombinant protein was detected by SDS-PAGE and Western blotting. The results showed that we successfully constructed the eukaryotic expression vector pUC57-BPI. Furthermore, porcine BPI recombinant protein was expressed in cultural supernatant of 293-6E cells in eukaryotic expression system, and the establishment of porcine BPI recombinant protein expression system in vitro provided the basis for the deep study of porcine BPI biological function and the preparation of porcine antibacterial protein.

Key words: porcine; BPI protein; eukaryotic expression

CLC Number:

YIN Xue-mei, XIA Ri-wei, SUN Li, ZHU Guo-qiang, BAO Wen-bin, WU Sheng-long. Construction and Eukaryotic Expression of Porcine BPI Protein Recombinant Vector[J]. , 2015, 42(4): 847-851.

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Construction and Eukaryotic Expression of Porcine BPI Protein Recombinant Vector

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