水貂奇异变形杆菌的分离鉴定及16S rRNA基因序列分析

doi:10.16431/j.cnki.1671-7236.2015.04.012

Abstract

Abstract: One strain pathogenic bacteria named PMSD was isolated from the infected mink in Liaoning province, and identified as Proteus mirabilis by morphological observation and VITEK 2 Compact System from bioMerieux. Antibiotic susceptibility test of PMSD showed that it was highly sensitive to aminoglycosides and quinolones while resistant to beta-lactam and sulfonamides. The 16S rRNA gene of 1 504 bp was amplified by PCR from PMSD strain, which was deposited in GenBank (accession No. KM229530). BLAST analysis showed that the gene shared more than 99% homology with the sequences of Proteus mirabilis. Furthermore, the phylogenetic tree analysis of 16S rRNA gene indicated that PMSD 16S rRNA gene shared higher homology with other 20 Proteus mirabilis strains available in GenBank. The homologies of nucleotide was from 98.9% to 99.7%. The highest homology was 99.7% with BB2000 strain, HI4320 strain, B1 strain and FCC141 strain. This study provided a foundation for the prevention and control of mink diseases caused by Proteus mirabilis.

Key words: mink; Proteus mirabilis; isolation and identification; 16S rRNA

CLC Number:

S852.61

WANG Jian-ke, CHENG Yue-ning, YI Li, ZHAO Hang, LIN Peng, TONG Ming-wei, CHENG Shi-peng. Isolation, Identification of Mink Proteus mirabilis and Sequence Analysis of 16S rRNA Gene[J]. , 2015, 42(4): 852-858.

References

[1] 段二珍,夏平安,张凤华,等.狐狸奇异变形杆菌的分离与鉴定[J].中国兽医科学,2008,38(12):1050-1054.
[2] 谌南辉,邓舜洲,郭松林,等.珍禽源奇异变形杆菌分离株微生物学特征研究[J].中国预防兽医学报,1999,21(4):2-5.
[3] 高正琴,王春玲,邢进,等.鼠奇异变形杆菌的鉴别诊断[J].实验动物科学与管理,2005,22(4):46-49.
[4] 胡传伟,贾赟,简中友,等.水貂奇异变形杆菌的分离与鉴定[J].经济动物学报,2006,10(2):77-79.
[5] 史同瑞,李丹,刘宇,等.貂奇异变形杆菌的分离及其生物学鉴定[J].中国预防兽医学报,2013,35(10):817-820.
[6] 潘玉善,苑丽,刘建华,等.鸡大肠杆菌和奇异变形杆菌的分离、鉴定及药敏分析[J].江西农业学报,2009,21(12):156-159.
[7] 朱明华,朱瑞良,马荣德,等.鸡奇异变形杆菌的分离鉴定和16S rRNA基因序列测定与系统进化分析[J].中国兽医学报,2011,31(6):804-808.
[8] 陈曦,王远微,王永,等.山羊腹泻粪便中奇异变形杆菌的分离鉴定及系统发育分析[J].中国畜牧兽医,2014,41(8):215-218.
[9] 赵振鹏,杨振,林伟东,等.猪源奇异变形杆菌的分离鉴定及集群运动分析[J].中国畜牧兽医,2014,41(10):219-224.
[10] 赵远,杨凤梅,陈丽雄,等.人工哺育期腹泻婴猴奇异变形杆菌的分离与鉴定[J].中国比较医学杂志,2012,22(4):19-22.
[11] 王瑞君,熊筱娟.棘胸蛙烂皮病奇异变形杆菌的分离、鉴定及对药物敏感性研究[J].淡水渔业,2012,42(4):31-34.
[12] 张庆华,熊清明,肖琳琳,等.大黄鱼溃烂症的一种致病菌——奇异变形杆菌ZXS02菌株[J].水产学报,2005,29(6):824-830.
[13] 刘海泉,赵强,孙晓红,等.多重PCR快速检测食品中的单核细胞增生性李斯特菌[J].中国农业科学,2010,43(23):4893-4900.
[14] 孙燕萍,彭浩,凌霞,等.VITEK 2 Compact全自动微生物分析系统的应用及鉴定结果分析[J].现代预防医学,2010,37(20):3891-3893.
[15] 乔宁,喻华,殷琳,等.VITEK 2 COMPACT全自动微生物分析仪性能分析[J].淮海医药,2012,30(3):211-213.
[16] Vinogradov E,Perry M B.Structural analysis of the core region of lipopolysaccharides from Proteus mirabilis serotypes O6,O48 and O57[J].European Journal of Biochemistry/FEBS,2000,267(8):2439-2446.

Isolation, Identification of Mink Proteus mirabilis and Sequence Analysis of 16S rRNA Gene

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[3]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[4]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[5]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[6]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[7]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[8]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[9]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[10]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.
[11]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.
[12]	XI Jing, YI Jihai, WANG Yueli, XU Zhenyu, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu. Isolation, Identification and Genotype Analysis of Group A Bovine Rotavirus G10 Strain XJ-2022 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3530-3538.
[13]	LIU Xiaolan, LIU Changjin, YU Wenyang, LI Xiaoxiang, BIAN Yanchao, HUANG Xiaohua, LUO Feng, DENG Shunzhou. Isolation and Identification of Porcine Rotavirus Jiangxi Strain AY01 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3151-3162.
[14]	XU Minsheng, KE Haiyi, SHI Keda, YANG Dongxia, ZHAI Shaolun, ZANG Yingan, LI Chunling. Isolation and Identification of Actinobacillus pleuropneumoniae from Guangdong Province and Detection and Analysis of Drug Resistance Phenotype and Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3212-3225.
[15]	YANG Zemin, LI Shuang, JIN Zhengyu, LIAO Yixiao, YANG Ying, WEN Ming. Isolation,Identification and Biological Characteristics of a Strain of Lactobacillus plantarum from Carp [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2805-2811.