凝结芽孢杆菌对感染产气荚膜梭菌肉鸡生长性能、肠道病变及免疫器官指数的影响

doi:10.16431/j.cnki.1671-7236.2015.02.038

Abstract

Abstract: This study was conducted to investigate the effect of dietary Bacillus coagulans on growth performance,intestinal lesions and immune organs index of broilers infected with Clostridium perfringens.A total of 312 Kebao 500 male broilers were randomly divided into 4 groups with 6 replicates per group, and 13 birds per replicate.The 4 treatment groups were an uninfected Clostridium perfringens group,an infected Clostridium perfringens group and the level supplemented with Bacillus coagulans at 0 and 400 mg/kg of feed using a 2×2 factorial completely randomized design.The whole experiment period was 35 days.The results showed that Clostridium perfringens infection decreased the ADG at days 15 to 35 (P<0.05),severed the necrotic enteritis-related lesions at day 28 (P<0.01),reduced the bursa index at day 35 (P<0.01).Bacillus coagulans supplementation increased the ADG and decreased the F/G at days 15 to 35(P<0.05),improved the lesion score (P<0.05),increased the thymus index at day 28 and the bursa index at day 35 (P<0.01).In the case of necrotic enteritis-related lesion,there was significantly interacted between Clostridium perfrigens and Bacillus coagulans.In conclusion,dietary supplementation of Bacillus coagulans could promote growth performance,improve immune index and enhance immune function against necrotic enteritis of broilers infected with Clostridium perfrigens.

Key words: Bacillus coagulans; Clostridium perfrigens; growth performance; intestinal lesions; immune organs index; broilers

CLC Number:

S831

GONG Xiu-yan, LIU Shun-de, WEI Ming, JIANG Qiu-fei. Effect of Bacillus coagulans on Growth Performance,Intestinal Lesions and Immune Organs Index of Broilers Infected with Clostridium perfringens[J]. , 2015, 42(2): 478-486.

References

[1] 孔庆娟.产气荚膜梭菌α毒素的研究进展[J].中国畜牧兽医,2011,38(1):233-235.
[2] Keyburn A L,Boyce J D,Vaz P,et al. NetB,a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens[J].PLoS Pathogens,2008,4(2):e26.
[3] Palliyeguru M,Rose S P,Mackenzie A M. Effect of dietary protein concentrates on the incidence of subclinical necrotic enteritis and growth performance of broiler chickens[J].Poultry Science,2010,89(1):34-43.
[4] Van Immerseel F,Rood J I,Moore R J,et al. Rethinking our understanding of the pathogenesis of necrotic enteritis in chickens[J].Trends in Microbiology,2009,17(1):32-36.
[5] Lensing M,van der Klis J D,Fabri T,et al. Efficacy of a lactylate on production performance and intestinal health of broilers during a subclinical Clostridium perfringens infection[J].Poultry Science,2010,89(11):2401-2409.
[6] McDevitt R M,Brooker J D,Acamovic T,et al. Necrotic enteritis;A continuing challenge for the poultry industry[J].World's Poultry Science Journal,2006,62(2):221-247.
[7] Grimes J L,Rahimi S,Oviedo E,et al. Effects of a direct-fed microbial (primalac) on turkey poult performance and susceptibility to oral Salmonella challenge[J].Poultry Science,2008,87(7):1464-1470.
[8] Mountzouris K C,Tsitrsikos P,Palamidi I,et al. Effects of probiotic inclusion levels in broiler nutrition on growth performance,nutrient digestibility,plasma immunoglobulins,and cecal microflora composition[J].Poultry Science,2010,89(1):58-67.
[9] Apata D F.Growth performance,nutrient digestibility and immune response of broiler chicks fed diets supplemented with a culture of Lactobacillus bulgaricus[J].Journal of the Science of Food and Agriculture,2008,88(7):1253-1258.
[10] Li L L,Hou Z P,Li T J,et al. Effects of dietary probiotic supplementation on ileal digestibility of nutrients and growth performance in 1- to 42-day-old broilers[J].Journal of the Science of Food and Agriculture,2008,88(1):35-42.
[11] Mountzouris K C,Tsirtsikos P,Kalamara E,et al.Evaluation of the efficacy of a probiotic containing Lactobacillus,Bifidobacterium,Enterococcus,and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities[J].Poultry Science,2007,86(2):309-317.
[12] Yu B,Liu J R,Hsiao F S,et al. Evaluation of Lactobacillus reuteri Pg4 strain expressing heterologous β-glucanase as a probiotic in poultry diets based on barley[J].Animal Feed Science and Technology,2008,141(1):82-91.
[13] Chichlowski M,Croom W J,Edens F W,et al. Microarchitecture and spatial relationship between bacteria and ileal,cecal,and colonic epithelium in chicks fed a direct-fed microbial,primaLac,and salinomycin[J].Poultry Science,2007,86(6):1121-1132.
[14] Li Y,Qin Y,Zhou X,et al. Comparative study on biological characteristics of six Bacillus strains[J].China Animal Husbandry & Veterinary Medicine,2011,4:16.
[15] Peng L,Li Z R,Green R S,et al. Butyrate enhances the intestinal barrier by facilitating tight junction assembly via activation of AMP-activated protein kinase in Caco-2 cell monolayers[J].The Journal of Nutrition,2009,139(9):1619-1625.
[16] Resta-Lenert S,Barrett K E. Live probiotics protect intestinal epithelial cells from the effects of infection with enteroinvasive Escherichia coli (EIEC)[J].Gut,2003,52(7):988-997.
[17] 崔云龙,万阜昌,李长龄,等.凝结芽胞杆菌(TBC169)片治疗便秘的实验研究和临床疗效[J].中国微生态学杂志,2006,18(4):264-265.
[18] Ara K,Meguro S,Hase T,et al. Effect of spore-bearing lactic acid-forming bacteria (Bacillus coagulans SANK 70258) administration on the intestinal environment,defecation frequency,fecal characteristics and dermal characteristics in humans and rats[J].Microbial Ecology in Health and Disease,2002,14(1):4-13.
[19] Wu S B,Rodgers N,Choct M. Optimized necrotic enteritis model producing clinical and subclinical infection of Clostridium perfringens in broiler chickens[J].Avian Dis,2010,54(3):1058-1065.
[20] Zhang H F,Zhang Z Y. Nutrition parameters and feeding standard for animals[M].Beijing:China Agricultural Press,1998.
[21] Gholamiandehkordi A R,Timbermont L,Lanckriet A,et al. Quantification of gut lesions in a subclinical necrotic enteritis model[J].Avian Pathol,2007,36(5):375-382.
[22] 曹国文,曾代勤,戴荣国,等.畜禽微生物饲料添加剂的研究与应用[J].饲料工业,2009,10:10-12.
[23] Zhou X,Wang Y,Gu Q,et al. Effect of dietary probiotic,Bacillus coagulans,on growth performance,chemical composition,and meat quality of Guangxi Yellow chicken[J].Poultry Science,2010,89(3):588-593.
[24] Hung A T,Lin S Y,Yang T Y,et al. Effects of Bacillus coagulans ATCC 7050 on growth performance,intestinal morphology,and microflora composition in broiler chickens[J].Animal Production Science,2012,52(9):874-879.
[25] 谢丽曲,詹湉湉,陈婉如,等.凝结芽孢杆菌对1~21日龄樱桃谷肉鸭生长性能、血清抗氧化指标和免疫器官指数的影响[J].福建畜牧兽医,2013,35(6):5-8.
[26] 董惠钧,姜俊云,郑立军,等.新型微生态益生菌凝结芽孢杆菌研究进展[J].食品科学,2010,31(1):292-294.
[27] 苏连朋,庄彦华,王加良,等.凝结芽孢杆菌联合奥沙拉嗪对轻中型溃汤性结肠炎患者血清TNF-α、IL-8和IL-17水平影响[J].中国微生态学杂志,2013,25(7):816-818.
[28] 赵宇亮,张聪敏,往琳,等.产气荚膜梭菌α毒素的研究进展[J].安徽农业科学,2013,41(12):5378-5380.
[29] Teo A Y,Tan H M. Inhibition of Clostridium perfringens by a novel strain of Bacillus subtilis isolated from the gastrointestinal tracts of healthy chickens[J].Appl Environ Miceobiol,2005,71(8):4185-4190.
[30] 李飞.凝结芽孢杆菌对噁唑酮诱导的大鼠溃疡性结肠炎的治疗作用研究[D].青岛:青岛科技大学,2011.
[31] 赵树平,包维臣,高鹏飞,等.凝结芽孢杆菌的特性及研究进展[J].家畜生态学报,2014,35(2):6-10.
[32] Zhang Z F,Cho J H,Kim I H. Effects of Bacillus subtilis UBT-MO2 on growth performance,relative immune organ weight,gas concentration in excreta,and intestinal microbial shedding in broiler chickens[J].Livestock Science,2013,155(2-3):343-347.
[33] 张高娜,张建梅,谷巍.凝结芽孢杆菌的特性与研究进展[J].饲料广角,2013(6):34-36.
[34] 马明颖,钟权,于永军.微生态制剂对雏鸡生长性能及免疫功能的影响[J].黑龙江畜牧兽医,2011,3:67-68.

Effect of Bacillus coagulans on Growth Performance,Intestinal Lesions and Immune Organs Index of Broilers Infected with Clostridium perfringens

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