猪博卡病毒非结构蛋白NP1基因的克隆及基因分型研究

doi:10.16431/j.cnki.1671-7236.2015.02.004

Abstract

Abstract: In order to demonstrate the nonstructural proteins 1 (NP1) molecular characterization,the target fragments was obtained from post-weaning multisystemic wasting syndrome piglets using PCR method with specific primers,targeting to the NP1 fragments.The results showed that the porcine bocavirus NP1 gene was 675 bp in length,coding an open reading frame (ORF) with 224 amino acids.The nucleotide sequence were compared with the porcine bocavirus NP1 downloaded from GenBank using MegAlign.The sequenced gene shared the highest homologies of nucleotides with the USA porcine bocavirus OH110 strain at 97.3%,and only shared 81.8% identity to IN109-1 strain; which shared the homogeneity with the United Kingdom porcine bocavirus F41 strain and 64-1 strain at 82.5% and 80.9%,respectively.However,the cloned sequence only shared the homologies of nucleotides with porcine bocavirus H18 strain at 43.8%.According to the phylogenetic tree,the porcine bocavirus could be divided into three genotypes,all of which the homologies of nucleotides were lower than 50.0%.The results suggested that the porcine bocavirus could be divided into three different genus in genus bocavirus.

Key words: porcine bocavirus; nonstructural proteins 1 (NP1); clone; sequence analysis; genotyping classification

CLC Number:

S852.65

CHEN Ru-jing, WU Xue-min, CHE Yong-liang, WANG Long-bai, YAN Shan, WEI Hong, LIU Yu-tao, ZHOU Lun-jiang. Cloning and Genotyping Classification of Nonstructural Protein NP1 Gene of Porcine Bocavirus[J]. , 2015, 42(2): 292-297.

References

[1] Blomström A,Belák S,Fossum C,et al.Detection of a novel porcine boca-like virus in the background of porcine circovirus type 2 induced postweaning multisystemic wasting syndrome[J].Virus Res,2009,146(1-2):125-129.
[2] Zeng S,Wang D,Fang L,et al.Complete coding sequences and phylogenetic analysis of porcine bocavirus[J].J Gen Virol,2011,92(4):784-788.
[3] Li B,Ma J,Xiao S,et al.Complete genome sequence of a novel species of porcine bocavirus,PBoV5[J].J Virol,2012,86(2):1286-1287.
[4] 李建宁,姚青,孙玉宁,等.博卡病毒属基因组特征与致病的分子机制[J].微生物学报,2013,53(5):421-428.
[5] 覃绍敏,吴健敏,马琳,等.猪博卡病毒全基因组序列分析与基因分型研究[J].中国预防兽医学报,2014,36(2):150-153.
[6] Cadar D,Cságola A,Lorincz M,et al.Genetic detection and analysis of porcine bocavirus type 1 (PoBoV1) in European wild boar (Sus scrofa)[J]. Virus Genes,2011,43(3):376-379.
[7] 翟少伦,陈胜男,魏文康,等.猪博卡病毒的研究进展[J].病毒学报,2012,28(2):190-193.
[8] Lau S,Woo P,Yip C,et al.Co-existence of multiple strains of two novel porcine bocaviruses in the same pig,a previously undescribed phenomenon in members of the family parvoviridae,and evidence for inter- and intra-host genetic diversity and recombination[J]. J Gen Virol,2011,92(9):2047-2059.
[9] Yang W,Yu J,Li J,et al.Genome characterization of a novel porcine bocavirus[J]. Arch Virol,2012,157(11):2125-2132.
[10] Jiang Y,Xiao C,Yin S,et al.High prevalence and genetic diversity of porcine bocaviruses in pigs in the USA,and identification of multiple novel porcine bocaviruses[J].J Gen Virol,2014,95(2):453-465.
[11] McKillen J,McNeilly F,Duffy C,et al.Isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in Northern Ireland[J].Vet Microbiol,2011,152(1-2):39-45.
[12] Wang E,Liu W,Yang B,et al.Complete sequence and phylogenetic analysis of a porcine bocavirus strain swBoV CH437[J].Virus Genes,2014,48(2):387-390.
[13] Shan T,Lan D,Li L,et al.Genomic characterization and high prevalence of bocaviruses in swine[J].PLoS One,2011,6(4):e17292.
[14] Cheng W,Li J,Huang C,et al.Identification and nearly full-length genome characterization of novel porcine bocaviruses[J].PLoS One,2010,5(10):e13583.
[15] Chen K,Shull B,Moses E,et al.Complete nucleotide sequence and genome organization of bovine parvovirus [J]. J Virol,1986,60(3):1085-1097.
[16] Wang X,Bai A,Zhang J,et al.A new nanoPCR molecular assay for detection of porcine bocavirus[J].J Virol Methods,2014,202:106-111.
[17] Li B,Xiao S,Ma J,et al.Development of a novel TaqMan-based real-time PCR assay for the detection of porcine boca-like virus (Pbo-likeV)[J]. Virol J,2011,8:357.
[18] Li B,Ma J J,Xiao S B,et al.Development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus[J].J Virol Methods,2012,179(2):390-395.

Cloning and Genotyping Classification of Nonstructural Protein NP1 Gene of Porcine Bocavirus

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