硫化氢的生成及其在细胞凋亡和炎性反应中的作用

doi:10.16431/j.cnki.1671-7236.2018.12.012

摘要/Abstract

摘要：

硫化氢（H₂S）是一种无色具有臭味的气体，不仅是畜禽舍内的一种有毒有害物质，而且近年来发现H₂S是继NO、CO之后第3种气体信号分子。H₂S在胃肠中可以通过沙门氏菌、幽门螺旋杆菌等对含硫有机物及硫酸盐分解和还原产生，也可以通过胱硫醚-β-合成酶（CBS）、胱硫醚-γ-裂解酶（CSE）、半胱氨酸转移酶（CAT）与3-巯基丙酮酸硫转移酶（3-MST）等催化L-半胱氨酸等含硫氨基酸产生。H₂S具有多种生理病理学作用，可通过阻断细胞色素C氧化酶而抑制线粒体呼吸（高浓度情况下），造成细胞凋亡，同时也能下调BCL-2及NF-κB等抗凋亡基因表达，促进细胞凋亡；可通过调节Caspase3而抑制细胞凋亡（低浓度情况下），还可以与NF-κB的P65发生巯基化反应促进下游抗凋亡基因表达而发挥抗凋亡作用。高浓度的H₂S可以上调NF-κB、P38、ERKE1/2等基因表达，加重炎症反应；低浓度H₂S可以通过抑制NF-κB信号通路发挥抗炎作用。作者通过综述H₂S气体的生成及其在细胞凋亡和炎性反应中的双重作用，为深入了解H₂S在疾病发展过程中的生理、病理机制提供参考。

关键词: 硫化氢(H₂S); 生成; 细胞凋亡; 炎性反应

Abstract:

H₂S is a colorless and odorous gas,it is not only a toxic and harmful substance in livestock houses,but also is the third gas signal molecule after NO and CO.H₂S can be produced through decompositing and reducting of sulfur-containing organic compounds and sulfates by Salmonella,Helicobacter pylori,etc.in the gastrointestinal tract,and also through catalyzing sulfur-containing amino acid such as L-cysteine by cystathionine-β-synthase (CBS),cystathionine-γ-lyase (CSE),cysteine transferase (CAT) and 3-mercapto-pyruvate thiotransferase (3-MST).H₂S has a variety of physiological and pathological effects.It can inhibit mitochondrial respiration and blocks apoptosis through blocking cytochrome C (at high content),while also down-regulates anti-apoptotic genes such as BCL-2 and NF-κB to promote apoptosis.It can inhibit apoptosis by regulating Caspase3 (at low content),and also can play a thiolation reaction with NF-κB p65 to promote the expression of downstream anti-apoptotic genes and play an anti-apoptotic effect.High concentration of H₂S could up-regulate the expression of NF-κB,P38 and ERKE1/2 genes to promote inflammatory responses,low concentrations of H₂S could exert anti-inflammatory effects through inhibiting NF-κB signaling pathway.Summarizing the formation of H₂S gas and its dual roles in apoptosis and inflammatory response,this paper provides a reference for the understanding of the physiopathological mechanism of H₂S in the process of disease development.

Key words: H₂S; generation; cell apoptosis; inflammatory reaction

中图分类号:

S852.21

张成玉, 王月影, 许春梅, 朱河水, 钟凯, 李和平. 硫化氢的生成及其在细胞凋亡和炎性反应中的作用[J]. 《中国畜牧兽医》, 2018, 45(12): 3402-3407.

ZHANG Chengyu, WANG Yueying, XU Chunmei, ZHU Heshui, ZHONG Kai, LI Heping. Generation of H₂S and Its Role in Apoptosis and Inflammatory Response[J]. , 2018, 45(12): 3402-3407.

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