龙须菜对异育银鲫消化吸收、免疫及抗氧化性能的影响

doi:10.16431/j.cnki.1671-7236.2018.06.013

摘要/Abstract

摘要：

本试验旨在评估龙须菜替代饲料中的菜粕对异育银鲫幼鱼消化吸收、免疫及抗氧化性能的影响，确定龙须菜的适宜替代范围。选取健康且规格一致的异育银鲫幼鱼450尾，随机分为6组，每组3个重复，每个重复25尾，以不含龙须菜的基础饲料为对照组饲料，以3%（D1）、6%（D2）、9%（D3）、12%（D4）、15%（D5）龙须菜替代菜粕配制成的饲料为试验饲料饲喂试验鱼8周，所有饲料等蛋等脂，试验结束时测定各组异育银鲫消化吸收、免疫及抗氧化相关酶活性。结果显示，与对照组相比，投喂龙须菜饲料各组异育银鲫肝脏消化酶活性无显著差异（P<0.05）；D1组异育银鲫前肠碱性磷酸酶、肝脏超氧化物歧化酶活性，D1和D2组异育银鲫前肠γ-谷氨酰转肽酶活性显著升高（P<0.05），D5组异育银鲫前肠脂肪酶、碱性磷酸酶活性和肝脏超氧化物歧化酶活性显著下降（P<0.05）；D1~D3组异育银鲫肾脏酸性磷酸酶活性、D2~D5组异育银鲫血清溶菌酶活性及D2、D3组异育银鲫血清总胆固醇含量和前肠总抗氧化能力显著升高（P<0.05），而D5组异育银鲫血清总蛋白含量显著下降（P<0.05）。综上所述，饲料中添加3%~6%的龙须菜可在一定程度上提高异育银鲫消化吸收与免疫性能，当龙须菜替代水平达15%时，会对鱼体消化吸收和健康产生负面影响。

关键词: 龙须菜; 异育银鲫; 消化吸收; 免疫; 抗氧化性能

Abstract:

The objective of this study was to evaluate the effects of the diets which used the Gracilaria lemaneiformis to substitute the rapeseed meal on the digestive absorption, immunity and antioxidant performance of juvenile Carassius auratus gibelio,to determine the optimal replacement scope of the Gracilaria lemaneiformis.In this study, 450 tail of uniform juvenile Carassius auratus gibelio were randomly divided into 6 groups with 3 replicates per group and 25 fish per replicate.The fish of the control group were fed with the basic diet without Gracilaria lemaneiformis,the test diets which used the 3% (D1),6% (D2),9% (D3), 12% (D4) and 15%(D5) Gracilaria lemaneiformis to substitute the rapeseed meal were used to feed the experimental fish for 8 weeks,respectively.The protein and fat content of all the diets were equal,and at the end of the experiment,the activities of enzyme involved in digestive absorption, immunity and antioxidant function were determined.The results indicated that compared with the control group, there were no significant differences in the activities of digestive enzymes in the liver of the fish from each group (P<0.05).The activities of the alkaline phosphatase (ALP) and the superoxide dismutase (SOD) in the liver of the fish from D1 group, the activities of the γ-glutamyl-transferase (γ-GT) in the anterior intestine of the fish from D1 and D2 groups were significantly increased (P<0.05),while the activities of the lipase (LPS),ALP and SOD of the fish from D5 group were decreased significantly (P<0.05).The activities of the acid phosphatase (ACP) in the kidney of the fish from D1 to D3 groups, the activities of serum lysozyme of the fish from D2 to D5 groups and the serum total cholesterol (TC) content and the total antioxidant capacities (T-AOC) in the anterior intestine of the fish from D2 and D3 groups were significantly increased (P<0.05),while the serum total protein (TP) content of the fish from D5 group were decreased greatly (P<0.05).The results indicated that a small amount addition of the Gracilaria lemaneiformis (3% to 6%) would improve the digestion,absorption and immune function of the Carassius auratus gibelio to some extent, when the substitute amount of the Gracilaria lemaneiformis in the diet up to 15%,the negative impacts on the digestive and absorption capacities as well as the health status of the fish would appear.

Key words: Gracilaria lemaneiformis; Carassius auratus gibelio; digestion and absorption; immunity; antioxidant performance

中图分类号:

S963

宣雄智, 李文嘉, 卢玉标. 龙须菜对异育银鲫消化吸收、免疫及抗氧化性能的影响[J]. 《中国畜牧兽医》, 2018, 45(6): 1526-1534.

XUAN Xiongzhi, LI Wenjia, LU Yubiao. Effects of Gracilaria lemaneiformis in Diets on Digestion and Absorption, Immunity and Antioxidant Performance of Juvenile Carassius auratus gibelio[J]. , 2018, 45(6): 1526-1534.

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