›› 2012, Vol. 39 ›› Issue (2): 114-116.

• 遗传繁育 • 上一篇    下一篇

湖羊瘦素基因内含子2多态性及其与体重性状的相关性分析

吴丽丽1,2, 赵晓枫2, 陈哲2, 张立凡2, 蒋晓玲2, 吕尊周2, 徐宁迎2   

  1. 1. 海南大学农学院,海南海口 570228;2. 浙江大学动物科学学院,浙江杭州 310058
  • 收稿日期:2011-06-17 修回日期:1900-01-01 出版日期:2012-02-20 发布日期:2012-02-20
  • 通讯作者: 徐宁迎

The Polymorphism of leptin Gene Intron 2 in Hu Sheep and its Influence on the Body Weight Traits

WU Li-li1,2, ZHAO Xiao-feng2, CHEN Zhe2, ZHANG Li-fang2, JIANG Xiao-ling2, LV Zun-zhou2, XU Ning-ying2   

  1. 1. College of Agriculture, Hainan University, Haikou 570228, China;2. College of Animal Science, Zhejiang University, Hangzhou 310058, China
  • Received:2011-06-17 Revised:1900-01-01 Online:2012-02-20 Published:2012-02-20

摘要: 利用测序和PCR-RFLP技术对85只湖羊个体瘦素(leptin)基因第2内含子进行多态性分析,检测到A99G和C150T两个SNPs,两座位的优势基因型均为野生基因型,基因型频率分别为0.7882和0.8000,突变纯合型均未检测到;卡方检验结果表明,两座位的基因型频率处于哈代—温伯格平衡(P>0.05);统计分析结果表明,A99G和C150T座位的不同基因型与初生重、3月龄体重、6月龄体重之间的相关关系不显著(P>0.05)。

关键词: 湖羊; leptin基因; SNP; 体重性状; 相关分析

Abstract: Sequencing and PCR-RFLP were applied to detect single nucleotide polymorphism (SNP) in intron 2 of leptin gene in 85 Hu sheeps. Two SNPs of A99G and C150T were found. The dominant genotypes in the two loci were both wild genotype,genotype frequencies were 0.7882 and 0.8000, respectively. Mutation genotype was not found in the both loci. Chi-square test showed that the population was at Hardy-Weinberg equilibrium(P>0.05). Association analysis between the SNPs and body weight traits showed that the genotypes on the two loci had no significant influence on birth weight, 3-month weight and 6-month weight (P>0.05).

Key words: Hu sheep; leptin gene; SNP; body weight traits; association analysis

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