野生乌苏里貉18SrDNA全序列的克隆测序及系统进化树分析

›› 2012, Vol. 39 ›› Issue (2): 117-120.

野生乌苏里貉18SrDNA全序列的克隆测序及系统进化树分析

刘诚刚, 杜智恒, 白秀娟

东北农业大学动物科学技术学院,黑龙江哈尔滨 150030

收稿日期:2011-07-04 修回日期:1900-01-01 出版日期:2012-02-20 发布日期:2012-02-20
通讯作者: 白秀娟

Cloning,Sequencing and Phylogenetic Analysis 18S rDNA Gene in Wide Nyctereutes Procyonoides Matschie

LIU Cheng-gang, DU Zhi-heng, BAI Xiu-juan

College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030,China

Received:2011-07-04 Revised:1900-01-01 Online:2012-02-20 Published:2012-02-20

摘要/Abstract

摘要： 试验采用聚合酶链式反应方法,从野生乌苏里貉耳部肉中提取DNA,扩增出18S rDNA的全序列,并对其进行克隆测序,获得长度为1831 bp的序列,并将序列提交到GenBank上,经BLAST分析结果表明,测序得到的乌苏里貉18S rDNA与其他物种同源性均在90%以上,说明18S rDNA基因具有较高的保守性,可以作为分类学当中系统进化分析的重要参考依据。

关键词: 野生乌苏里貉; 克隆测序; 18S rDNA; 系统进化树

Abstract: In this study, 18S rDNA gene was extracted from wild Nyctereutes procyonoides matschie's meat of ear, and amplified by polymerase chain reaction (PCR) method, then cloned and sequenced. A lengh of 1831 bp was cloned, the sequences had been sent to GenBank and registered, comparison of the sequence with that of other animals 18S rDNA gene was carried out through BLAST. The results showed that the homologies of the nucleotide sequence were above 90% with Nyctereutes procyonoides matschie, and 18S rDNA gene was conserved in evolution, which could be used as phylogenetic analysis of the important reference.

Key words: wild Nyctereutes procyonoides matschie; cloning and sequencing; 18S rDNA; phylogenetic

中图分类号:

Q813

刘诚刚;杜智恒;白秀娟. 野生乌苏里貉18SrDNA全序列的克隆测序及系统进化树分析[J]. , 2012, 39(2): 117-120.

LIU Cheng-gang;DU Zhi-heng;BAI Xiu-juan. Cloning,Sequencing and Phylogenetic Analysis 18S rDNA Gene in Wide Nyctereutes Procyonoides Matschie[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(2): 117-120.

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