拉合尔钝缘蜱的形态学及分子生物学鉴定

doi:10.16431/j.cnki.1671-7236.2017.04.030

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 1149-1158.doi: 10.16431/j.cnki.1671-7236.2017.04.030

拉合尔钝缘蜱的形态学及分子生物学鉴定

吕继洲¹, 赵丽², 赵昱林³, 韩雪清¹, 王慧煜¹, 吴绍强¹

1. 中国检验检疫科学研究院动物检疫研究所, 北京 100029;
2. 塔里木大学动物科学学院, 阿拉尔 843300;
3. 清华大学附属中学, 北京 100084

收稿日期:2016-09-05 出版日期:2017-04-20 发布日期:2017-05-03
通讯作者: 吴绍强 E-mail:sqwu@sina.com
作者简介:吕继洲(1984-),男,山东临清人,博士,副研究员,研究方向:动物寄生虫分子系统学,E-mail:ljzffff@163.com
基金资助:
国家科技支撑计划课题（2013BAD12B03、2013BAD12B01）

Morphological and Molecular Biology Identification of Ornithodoros lahorensis

LV Ji-zhou¹, ZHAO Li², ZHAO Yu-lin³, HAN Xue-qing¹, WANG Hui-yu¹, WU Shao-qiang¹

1. Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China;
2. College of Animal Science, Tarim University, Alar 843300, China;
3. Tsinghua University High School, Beijing 100084, China

Received:2016-09-05 Online:2017-04-20 Published:2017-05-03

摘要/Abstract

摘要：

试验旨在建立拉合尔钝缘蜱的分子生物学鉴定方法。从新疆地区绵羊体表采集寄生蜱，借助体视显微镜和电子显微镜对其进行形态学特征的准确鉴别，初步筛选出疑似拉合尔钝缘蜱。经PCR扩增、测序获得其18S rDNA、16S rDNA及线粒体色素氧化酶亚基Ⅰ基因（cytochrome oxidase Ⅰ，COⅠ）序列，结合GenBank数据库中参考序列，比对分析同源性并构建系统进化树，进行遗传距离评估。本研究全面、清晰地揭示了拉合尔钝缘蜱卵圆形背板、背部圆形盘窝等显微形态特征的细节。同时，在基于16S rDNA与COⅠ序列的邻位相接系统进化树中，拉合尔钝缘蜱分布于两大进化枝之一的软蜱科，同种聚类良好，且拉合尔钝缘蜱种内K2P遗传距离为0~0.3%，本研究方法可快速、准确鉴定拉合尔钝缘蜱物种。

关键词: 线粒体色素氧化酶亚基Ⅰ基因; 16S rDNA; 18S rDNA; 拉合尔钝缘蜱; 形态学特征; 分子鉴定

Abstract:

This study was to establish a method for the identification of Ornithodoros lahorensis with molecular biology. The samples of Ornithodoros lahorensis were collected from sheep in Xinjiang region and the morphological characters were screened by stereo microscopy and electronic microscopy. Then the 16S rDNA,18S rDNA and cytochrome oxidase Ⅰ(COⅠ) of samples were amplified by PCR and subsequently sequenced. Then the analysis of genetic divergences and Neighbor-Joining (NJ) phylogenetic tree were carried out based on the sequences acquired from Ornithodoros lahorensis,combined with the reference sequences of GenBank database. In this study,the comprehensive and clear micromorphological traits of ovoid scutum,round fovea of Ornithodoros lahorensis were provided. Besides,the DNA sequences of Ornithodoros lahorensis were assembled together with sequences of Argasidae and form monophyletic clades in the 16S rDNA and COⅠ based Neighbor-Joining trees. According to the intraspecific K2P genetic distances of Ornithodoros lahorensis (0~0.3%),we determined that the molecular biology method based on 16S rDNA and COⅠ could rapidly and accurately identify the species of Ornithodoros lahorensis.

Key words: COⅠ; 16S rDNA; 18S rDNA; Ornithodoros lahorensis; morphological characters; molecular identification

中图分类号:

S852.7

吕继洲, 赵丽, 赵昱林, 韩雪清, 王慧煜, 吴绍强. 拉合尔钝缘蜱的形态学及分子生物学鉴定[J]. 《中国畜牧兽医》, 2017, 44(4): 1149-1158.

LV Ji-zhou, ZHAO Li, ZHAO Yu-lin, HAN Xue-qing, WANG Hui-yu, WU Shao-qiang. Morphological and Molecular Biology Identification of Ornithodoros lahorensis[J]. , 2017, 44(4): 1149-1158.

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拉合尔钝缘蜱的形态学及分子生物学鉴定

Morphological and Molecular Biology Identification of Ornithodoros lahorensis

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