贵州省山羊源流产细菌调查及致病机理初探

doi:10.16431/j.cnki.1671-7236.2015.10.039

›› 2015, Vol. 42 ›› Issue (10): 2779-2787.doi: 10.16431/j.cnki.1671-7236.2015.10.039

贵州省山羊源流产细菌调查及致病机理初探

王伟¹, 王开功^1,2, 周碧君^1,2, 王铭¹, 冯旭芳¹, 马光强¹, 罗意¹

1. 贵州大学动物科学学院, 贵阳 550025;
2. 贵州省动物疫病研究室, 贵阳 550025

收稿日期:2015-05-11 出版日期:2015-10-20 发布日期:2015-10-23
通讯作者: 王开功 E-mail:kaigongwang@aliyun.com
作者简介:王伟(1990-),男,内蒙古赤峰人,硕士生,研究方向:预防兽医学,E-mail:332087913@qq.com
基金资助:
贵州省农业科技攻关项目"山羊繁殖障碍性疫病防控关键技术研究与示范"黔科合NY字[2011-3105]号;贵州省重大科技专项"酒糟资源化循环利用模式关键技术研究与集成示范"黔科合重大专项字[2011]6021-4号;贵州省农业科技攻关项目"贵州省主要人畜共患病综合防控技术研究"黔科合NY字[2011]3061号;贵州省农业委员会科技计划项目"贵州省2015年重大动物疫病省级定点监测"(2015-01号)

Preliminary Study on Abortion Bacteria from Goat in Guizhou Province and their Pathogenic Mechanism

WANG Wei¹, WANG Kai-gong^1,2, ZHOU Bi-jun^1,2, WANG Ming¹, FENG Xu-fang¹, MA Guang-qiang¹, LUO Yi¹

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Laboratory for Animal Disease of Guizhou, Guiyang 550025, China

Received:2015-05-11 Online:2015-10-20 Published:2015-10-23

摘要/Abstract

摘要： 为调查贵州省细菌性山羊流产疫病的主要原因,分析山羊源流产细菌对正常怀孕动物免疫平衡的影响,本试验采集贵州省6个地区(州、市)12个规模养羊场临床健康和发病流产山羊的胎儿、子宫和母羊的阴道棉拭子样本共计107份进行细菌分离和鉴定,采用药敏纸片琼脂扩散法对3种主要羊源分离菌进行20种抗生素的耐药性调查,并用羊源流产细菌建立BALB/c小鼠流产模型,通过ELISA方法对妊娠相关细胞因子和激素进行测定。结果显示,从采集的107份样本中共分离到13个属细菌共302株,其中发病流产山羊生殖器官中分离的细菌种类多于临床健康山羊;流产山羊生殖器官中分离的羊源细菌耐药率高于临床健康山羊细菌耐药率;3种主要羊源流产细菌埃希氏菌、葡萄球菌和链球菌均可引起怀孕BALB/c小鼠发生不同程度的流产,流产率为埃希氏菌>葡萄球菌>链球菌;胚胎吸收率为埃希氏菌>葡萄球菌>链球菌;与空白对照组相比,各试验组BALB/c小鼠血清中的IFN-γ、IL-2含量上升,而IL-4、IL-10和孕酮含量降低。结果表明,贵州省细菌性山羊流产的发生与山羊生殖器官细菌种类多少、耐药性高低有一定相关性,推测山羊源流产细菌可能通过影响怀孕山羊的主要妊娠相关细胞因子和激素的含量导致山羊流产。

关键词: 山羊; 细菌; 耐药性; 细胞因子; 流产

Abstract: In order to study the main reason of bacterial goat abortion in Guizhou province,and analyze the bacteria impact of abortion goat immune balance in normal pregnancy animals,107 samples of the goat fetus,uterus and vaginal swabs were collected from clinical health and morbidity abortion goat in 12 scale goat farms of 6 areas in Guizhou province.The bacteria were isolated and identified.The drug susceptibility disc agar diffusion method was launched for the survey of the resistance of three main sources of goat isolates to 20 kinds of antibiotics.The BALB/c mice model of abortion was established using abortion bacteria isolated from goats.The pregnancy related hormones and cytokines were determined by ELISA method.The results showed that 302 strains bacteria of 13 genera were isolated from the samples,and the species of bacteria isolated from morbidity abortion goat were more than the clinical health goats.The bacteria resistant rate of goat reproductive organs in abortion goats was higher than that of clinical healthy goats bacteria.The varying degrees of abortion of BALB/c mice could cause by Escherichia,Staphylococcus and Streptococcus isolated from goats,the rates were Escherichia>Staphylococcus>Streptococcus,respectively and the embryo resorption rates were Escherichia>Staphylococcus>Streptococcus,respectively.Compared with the control group,IFN-γ and IL-2 in the serum were increased;However,IL-4,IL-10 and progesterone were decreased.The results indicated that the occurrence of bacterial goat abortion diseases had certainly related to the number of bacterial types and the level of drug resistance in goat reproductive organs.The levels of pregnancy related hormones and cytokines in goats serum could be changed by abortion bacteria,thus lead to goats abortion.

Key words: goat; bacteria; resistance; cytokines; abortion

中图分类号:

S852.61

王伟, 王开功, 周碧君, 王铭, 冯旭芳, 马光强, 罗意. 贵州省山羊源流产细菌调查及致病机理初探[J]. , 2015, 42(10): 2779-2787.

WANG Wei, WANG Kai-gong, ZHOU Bi-jun, WANG Ming, FENG Xu-fang, MA Guang-qiang, LUO Yi. Preliminary Study on Abortion Bacteria from Goat in Guizhou Province and their Pathogenic Mechanism[J]. , 2015, 42(10): 2779-2787.

参考文献

[1] 冉双存,靳文忠,李长宏,等.青海省英得尔种羊场种羊流产血清学调查[J].中国动物检疫,2010,27(6):54-55.
[2] Chis V,Porcu R,Tanda A.First isolation and characterization of Chlamydophila abortus from abortion tissues of sheep in Sardinia,Italy[J].Veterinaria Italiana,2013,49(4):331-334.
[3] Pereira M F,Peixoto R M,Piatti R M,et al.Occurrence and risk factors for Chlamydophila abortus infection in sheep and goats in Pernambuco[J].Pesq Vet Bras,2009,29(1):549-555.
[4] Sahina O,Fitzgeraldb C,Stroika S,et al.Molecular evidence for zoonotic transmission of an emergent,highly pathogenic Campylobacter jejuni clone in the United States[J].Journal of Clinical Microbiology,2012,50(3):3680-3687.
[5] 刘冬香,刘书亮.四川省动物性食品源金黄色葡萄球菌的耐药性分析[J].中国兽医杂志,2009,45(5):6-8.
[6] 刘芳,罗颂平.Th1/Th2 型细胞因子平衡调控对正常妊娠影响的研究进展[J].广东中医药大学学报,2004,21(3):231-233.
[7] Chaouat G,Diallo J T,Volumenie J L,et al.Immune suppression and Th1/Th2 balance in pregnancy revisited:A (very) personal tribute to Tom Wegmann[J].Am J Reprod Immunol,1997,37(6):427-434.
[8] 颜艾,王开功.贵州地区部分羊场主要疫病的流行情况调查[J].畜牧与兽医,2011,43(9):73-75.
[9] 陆承平.兽医微生物学[M].北京:中国农业出版社,2007.
[10] Chaouat G,Meliani A A,Martal J,et al.IL-10 prevents naturally occurring fetal loss in the CBA × DBA/2 mating combination,and local defect in IL-10 production in this abortion-prone combination is corrected by in vivo injection of IFN-tau[J].J Immunology,1995,154(9):4261-4268.
[11] 罗意,周碧君.贵州省山羊流产主要病原菌分离鉴定及致病性试验[J].广东农业科学,2013,15(3):134-136.
[12] 凌宗欣.女性生殖道微生物群落菌群多样性变化与生殖道感染的相关性研究[D].杭州:浙江大学,2012.
[13] Zhou X,Hansmann M A,Davis C C,et al.The vaginal bacterial communities of Japanese women resemble those of women in other of women racial group[J].FEMS Immunol Med Microbiol,2010,58(2):169-181.
[14] Tumbaugh P J,Ley R E,Hamady M.The human microbiome project:Exploring the microbial part of ourselves in a changing world[J].Nature,2007,449(7164):804-810.
[15] 马云燕.妊娠期阴道乳酸杆菌含量变化及干预的研究[D].广州:南方医科大学,2009.
[16] 康白.阴道正常茵群的定性及定量分析[J].中国微生态杂志,2001,13(4):191-193.
[17] 夏利宁.新疆某羊场分离的大肠杆菌对抗生素耐药性调查[J].新疆农业科学,2014,51(1):150-156.
[18] 付明哲,李长安,白涛,等.羊链球菌的自动鉴定和耐药性分析[J].西北农业学报,2014,23(1):1-5.
[19] 赵明秋,沈海燕,潘文,等.细菌耐药性产生的原因、机制及防治措施[J].中国畜牧兽医,2011,38(5):177-181.
[20] 吴秀群,王立志.热应激对奶牛血液中内毒素含量和抗氧化指标的影响[J].四川畜牧兽医,2011,38(1):22-23.
[21] Raghu Pathy R.Th1-type immunity is incompatible with successful pregnancy[J].Immunol Today,1997,18(10):476-482.
[22] 夏红飞,郝艳红,彭景楩.妊娠大鼠子宫与胎盘IL-1β表达及IFN-γ对其表达的影响[J].动物学报,2004,50(1):48-54.
[23] Nisula L K,Hirvonen M R,Roponen M,et al.Maternal and neonatal IL-4 and IFN-gamma production at delivery and 3 months after birth[J].J Reprod Immunol,2003,60(1):25-33.
[24] 张昱,王海东,贺俊平,等.妊娠相关糖蛋白及其在反刍动物早期妊娠诊断中的应用[J].中国畜牧兽医,2015,42(3):764-768.
[25] Szekeres-Bartho J,Balasch J.Progestagen therapy for recurrent miscarriage[J].Hum Reprod Update,2008,14(1):27-35.

贵州省山羊源流产细菌调查及致病机理初探

Preliminary Study on Abortion Bacteria from Goat in Guizhou Province and their Pathogenic Mechanism

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	吕春荣, 梁家充, 邵庆勇, 吴国权, 张玉梅, 权国波. 丙酮酸钠对山羊冷冻精子质量的影响[J]. 中国畜牧兽医, 2023, 50(6): 2363-2369.
[2]	李晓宇, 李若愚, 王晓, 王丽丽, 李纪彬, 李淑英, 徐永平. 丝状噬菌体对宿主菌适应性影响的研究进展[J]. 中国畜牧兽医, 2023, 50(6): 2496-2506.
[3]	邵永斌, 郑一闯, 张竞怡, 魏琳颖, 田勇, 孙国结, 刘雨然, 罗燕, 谷新利. 益母草碱对LPS诱导小鼠子宫内膜炎的抗炎作用研究[J]. 中国畜牧兽医, 2023, 50(5): 2149-2155.
[4]	王路才, 撒朗文朱, 张焕容. 禽源肠炎沙门菌的分离鉴定及耐药性与致病性分析[J]. 中国畜牧兽医, 2023, 50(4): 1663-1674.
[5]	曹颖颖, 梁亮, 曾怡蓉, 钟清华, 袁小芳, 唐海波, 冷静. 滇西树鼩源沙门氏菌的分离鉴定及生物学特性研究[J]. 中国畜牧兽医, 2023, 50(4): 1718-1728.
[6]	宋兴超, 孟金柱, 赵园园, 吴震洋, 安清明. 贵州白山羊MyoG基因启动子区序列克隆及生物信息学分析[J]. 中国畜牧兽医, 2023, 50(3): 893-903.
[7]	萨初拉, 吴铁成, 马跃军, 何云梅, 朱莉仙, 何托雅, 武玉平, 刘斌. 绒山羊4个多胎性状候选基因多态性及其与产羔数的关联分析[J]. 中国畜牧兽医, 2023, 50(3): 1037-1047.
[8]	朱琪琪, 张继瑜, 王承业, 吴灵玉, 王玮玮, 朱阵. 河北地区动物源大肠杆菌多重耐药性与流行性分析[J]. 中国畜牧兽医, 2023, 50(3): 1081-1092.
[9]	祝希辉, 李艳兰, 王志伟, 庞喆羽, 李玉保, 刘成, 司振书, 路建彪, 曹胜亮, 薛希娟. 1株鸭疫里默氏杆菌的全基因组测序及耐药性与遗传进化分析[J]. 中国畜牧兽医, 2023, 50(3): 1140-1149.
[10]	韦义荣, 郑自华, 张叁保, 宋颖, 蒋海玉, 孙雯玥, 程鹏飞, 刘雨帆, 邹剑伟, 黄艳娜, 潘艳, 蒋钦杨. 努比亚山羊UCP1基因克隆、生物信息学分析及组织表达研究[J]. 中国畜牧兽医, 2023, 50(2): 440-450.
[11]	秦志云, 姜怀志. 羊胎儿期皮肤毛囊发育及调控机制的研究概述[J]. 中国畜牧兽医, 2023, 50(2): 638-646.
[12]	王冰艺, 马弘财, 邹明昊, 樊世杰, 元振杰, 拉巴次仁, 董海龙, 曾江勇. 牦牛源链球菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(2): 745-753.
[13]	钟华晨, 王丽芳, 郭晨阳, 刘嘉琳, 宋洁. 内蒙古地区乳房炎奶样与环境中细菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(2): 817-826.
[14]	但一昕, 向华, 张焕容, 杨璐, 任玉鹏, 徐颂为, 何翃闳, 朱江江. 羊口疮病毒129蛋白互作宿主蛋白的筛选与C1QBP基因的克隆分析[J]. 中国畜牧兽医, 2023, 50(1): 1-14.
[15]	潘思尧, 张丹丹, 刘权辉, 王国栋, 吕丹薇, 黄奔. 抑制miR-142-3p对奶山羊化学诱导乳腺上皮细胞泌乳功能的影响[J]. 中国畜牧兽医, 2023, 50(1): 58-66.