《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (3): 696-700.doi: 10.16431/j.cnki.1671-7236.2015.03.029

• 疾病防治 • 上一篇    下一篇

曲靖地区仔猪腹泻细菌性病原的分离鉴定与耐药性分析

朱玲云1, 郑龙龙1, 毕峻龙2, 冯朝相3, 杨贵树1, 白卫兵1   

  1. 1. 云南农业大学动物科学技术学院, 昆明 650201;
    2. 云南楚雄州动物疫病预防控制中心, 楚雄 675000;
    3. 云南昭通巧家县乡镇农业综合服务中心, 昭通 654600
  • 修回日期:2014-12-08 出版日期:2015-03-20 发布日期:2015-03-30
  • 通讯作者: 白卫兵 E-mail:360536885@qq.com
  • 作者简介:朱玲云(1991-),女,云南保山人,硕士生,研究方向:动物传染病学与分子流行病学,E-mail:1402443495@qq.com
  • 基金资助:

    国家自然科学基金(31160509)

Isolation,Identification and Drug Resistance Analysis of Bacterial Pathogen of Piglets Diarrhea Syndrome in Qujing Area

ZHU Ling-yun1, ZHENG Long-long1, BI Jun-long2, FENG Chao-xiang3, YANG Gui-shu1, BAI Wei-bing1   

  1. 1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
    2. Yunnan Chuxiong Animal Disease Prevention and Control Center, Chuxiong 675000, China;
    3. Yunnan Zhaotong Qiaojia County Township Agricultural Service Centers, Zhaotong 654600, China
  • Revised:2014-12-08 Online:2015-03-20 Published:2015-03-30

摘要: 为了解曲靖地区仔猪出现腹泻的细菌性病因,对5个养殖场148份出现仔猪腹泻症状的样品进行细菌分离培养及耐药性分析,结果显示,检出致病性大肠杆菌(E.coli)114份,阳性率为77.03%;沙门氏菌(Salmonella)26份,阳性率为17.57%,混合感染率为17.57%.致病性大肠杆菌和沙门氏菌对多种抗生素产生了耐药性,其中114株致病性大肠杆菌中97株呈多重耐药性,占总菌数的85.09%,26株沙门氏菌中20株呈多重耐药性,占总菌数的76.92%.结果表明,致病性大肠杆菌是导致当地哺乳仔猪腹泻的主要细菌性病原,部分仔猪继发感染沙门氏菌,两种细菌对多种抗生素产生了耐药性,多重耐药现象较严重.

关键词: 仔猪腹泻; 大肠杆菌; 沙门氏菌; 耐药性

Abstract: In order to know the bacterial causes of piglet diarrhea in the Qujing area,the study collected 148 samples from 5 farms appearing diarrhea symptoms for bacterial isolation,culture and drug resistance analysis.The results showed that the detection of pathogenic E.coli was 114 strains,the positive rate was 77.03%;Salmonella was 26 strains,the positive rate was 17.57%,mixed infection rate was 17.57%.Pathogenic E.coli and Salmonella were resistant to a variety of clinic medicines.The 97 strains were multidrug resistance in 114 pathogenic E.coli,showing 85.09% of the total number of bacteria;20 of 26 Salmonella were multidrug resistant,showing 76.92% of the total number of bacteria.The results suggested that pathogenic E.coli was major bacterial pathogen which causing piglets diarrhea in Qujing local area,some piglets were secondary infected with Salmonella,two kinds of bacteria had both appeared high resistance,multidrug resistance were more serious.

Key words: piglets diarrhea; E.coli; Salmonella; drug resistance

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