关键词: 支气管败血波氏杆菌; 分离与鉴定; PCR; 药敏试验; 耐药基因

Abstract: In order to know the infection and drug resistance of Bordetella bronchiseptica from rabbit in Zhejiang province in recent years and guide the rational use of drugs,resistance genes were detected to study the possible mechanism of drug resistance.The bacteria were identified via the test of mophology,culture distinguishing and biochemical test combined with PCR method;K-B's paper method was used to detect the drug resistance rate for 18 kinds of common antibiotics;Specific primers were also designed to detect through PCR method.Twenty-two Bordetella bronchiseptica strains were isolated and identified during 2012-2014.Drug susceptibility results showed that bacteria were highly resistant to penicillin G,cefradine,streptomycin,lincomycin,clindamycin and sulfamnomethoxine,and sensitive to polymyxin,levofloxacin,doxycycline and tetracycline,etc;bla_TEM gene from the bacteria was detected positive which were resistant to beta-lactams.Rabbit respiratory disease was mainly caused by Bordetella bronchiseptica,the isolated bacteria were multi-drug resistance,drug resistance rate was high,the resistant genes were consistant with drug-resistant phenotype.

Key words: Bordetella bronchiseptica; isolation and identification; PCR; antibiotic sensitivity test; drug resistance gene