›› 2007, Vol. 34 ›› Issue (2): 67-68.

• 遗传繁育 • 上一篇    下一篇

改进的表面张力辅助去核法对小鼠卵母细胞去核的研究

李井春,方南洙
  

  1. 延边大学农学院动物遗传育种与繁殖实验室, 龙井 133400
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-20 发布日期:2007-02-20

Mouse Oocyte Enucleation with Modified Surface Tension Assisted Method

LI Jingchun, FANG Nanzhu   

  1. College of Agriculture,Yanbian University, Longjing 133400, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-20 Published:2007-02-20

摘要: 为了探讨改进的小鼠表面张力辅助去核法(STA)的可靠性,本试验用改进的表面张力辅助去核法与表面张力辅助去核法对小鼠卵母细胞进行去核操作,比较其去核操作效率。结果发现,改进的表面张力辅助去核法能显著减少去核操作时间,去核率与表面张力去核法无显著差异。改进的小鼠表面张力辅助去核法是一种适用于小鼠卵母细胞操作的快速、简单、实用的去核方法。

关键词: 小鼠; 改进的表面张力辅助去核法; 去核

Abstract: To study the reliability of the new enucleation method for mouse oocyte:modified surface tension assisted method. Modified surface tension assisted method is significantly better than STA’s method in the enucleated rates of time(P<0.05). Rate of Enucleation is similar between two groups. Modified surface tension assisted method is a efficient and simple enucleation method with the advantage of saving manipulating time and nondamage to mouse oocyte.

Key words: mouse; modified surface tension assisted method; enucleation

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