2种日粮对不同生长阶段乌金猪脂肪组织脂类合成代谢相关基因表达的影响

›› 2013, Vol. 40 ›› Issue (10): 21-25.

2种日粮对不同生长阶段乌金猪脂肪组织脂类合成代谢相关基因表达的影响

黄英¹, 李永能², 杨明华¹, 潘洪彬¹, 赵素梅¹, 高士争¹

1. 云南农业大学, 云南省动物营养与饲料重点实验室, 云南昆明 650201;
2. 云南农业大学招生就业处, 云南昆明 650201

收稿日期:2013-03-26 出版日期:2013-10-20 发布日期:2013-12-19
通讯作者: 赵素梅 E-mail:zhaosm2009@126.com
作者简介:黄英(1978-),女,云南人,硕士,研究方向:动物分子营养与代谢调控。李永能(1976-),男,云南人,硕士,研究方向:动物分子营养与代谢调控。黄英与李永能对本文具有同等贡献,并列为第一作者。
基金资助:
云南省自然科学基金重点项目(2005C0008Z)。

Effect of Two Diets on the Expression Levels of Lipid Synthesis Metabolism Related Genes in Adipose Tissue of Wujin Pigs with Different Growth Stage

HUANG Ying¹, LI Yong-neng², YANG Ming-hua¹, PAN Hong-bin¹, ZHAO Su-mei¹, GAO Shi-zheng¹

1. Yunnan Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming 650201, China;
2. Admission and Employment Office, Yunnan Agricultural University, Kunming 650201, China

Received:2013-03-26 Online:2013-10-20 Published:2013-12-19

摘要/Abstract

摘要： 为探讨不同日粮对乌金猪不同生长阶段脂类合成代谢相关基因表达的影响,试验选取体重约23 kg乌金猪36头,公、母各半,随机分为2个处理组,每个处理组3个重复,每个重复6头。处理1组饲喂乌金猪日粮,处理2组饲喂NRC日粮。分别于30、60和100 kg体重时屠宰,通过Real-time PCR比较研究3个脂肪合成代谢相关基因在脂肪组织中表达的差异。结果显示,体重30 kg时,处理1组脂肪酸合成酶(fatty acid synthase,FAS)的相对表达量显著低于处理2组(P<0.05),60和100 kg时,处理1组FAS基因的相对表达量高于处理2组,60 kg时达到显著水平(P<0.05);在各个体重时,处理1组固醇调节元件结合蛋白1c(sterol regulator element binding protein-1c,SREBP-1c)的相对表达量均高于处理2组,30和60 kg时达到显著水平(P<0.05);在各个体重时,处理1组二酰基甘油酰基转移酶1(diacylglycerol acyltransferase-1,DGAT-1)的相对表达量低于处理2组,100 kg时达到显著水平(P<0.05)。提示,不同日粮调控不同体重乌金猪脂类合成代谢相关基因表达存在着差异。

关键词: 乌金猪; 脂肪组织; 脂类合成代谢; 基因表达

Abstract: The aim of the study was to investigate the effects of different diets on expression levels of lipid synthesis metabolism related genes in Wujin pigs. Thirty-six Wujin pigs with the body weights of 23 kg (female and male half and half) were randomly assigned into two groups with three replicates per group and six heads in each replicate. The treatment 1 group was fed with the Wujin diet, and the treatment 2 group was fed with the NRC diet. At the body weight of 30, 60 and 100 kg, pigs were slaughtered and adipose tissue samples were taken for analyzing the expression levels of three lipid metabolism genes using Real-time PCR technology. The results showed that at the body weight of 30 kg, the expression levels of FAS gene in treatment 1 group were dramatically lower than that in the treatment 2 group (P<0.05). At the body weights of 60 and 100 kg, the expression levels of FAS gene in treatment 1 group were higher than that in the treatment 2 group, but it was significantly different at the body weight of 60 kg (P<0.05). At the body weights of 30, 60 and 100 kg, the expression levels of SREBP-1c gene in treatment 1 group were higher than the treatment 2 group, but the difference was significant at the body weights of 30 and 60 kg (P<0.05). At the body weights of 30, 60 and 100 kg, the expression levels of DGAT-1 gene in treatment 1 group were lower than the treatment 2 group, but the difference was significant at the body weight of 100 kg (P<0.05).The results indicated that the diets could differently regulate the expression of lipid synthesis metabolism related genes in porcines with various body weights.

Key words: Wujin pigs; adipose tissue; lipid synthesis metabolism; gene expression

中图分类号:

Q344⁺.13

黄英, 李永能, 杨明华, 潘洪彬, 赵素梅, 高士争. 2种日粮对不同生长阶段乌金猪脂肪组织脂类合成代谢相关基因表达的影响[J]. , 2013, 40(10): 21-25.

HUANG Ying, LI Yong-neng, YANG Ming-hua, PAN Hong-bin, ZHAO Su-mei, GAO Shi-zheng. Effect of Two Diets on the Expression Levels of Lipid Synthesis Metabolism Related Genes in Adipose Tissue of Wujin Pigs with Different Growth Stage[J]. , 2013, 40(10): 21-25.

参考文献

1 王忠庆,荣耀先.大河乌猪与大河猪(乌金猪)肉质性状研究[J].云南农业科技,2004(z1):41~44.
2 杨明华,李永能,黄英,等.日粮蛋白质水平对乌金猪肝脏组织脂类代谢相关基因表达的影响[J].中国畜牧兽医,2013,40(2):108~112.
3 葛长荣,赵素梅,张曦,等.不同日粮蛋白质水平对乌金猪肉品质的影响[J].畜牧兽医学报,2008,39(12):1692~1700.
4 潘洪彬,赵素梅,黄英,等.饲粮能量水平对乌金猪脂肪组织脂类合成代谢相关基因表达的影响[J].动物营养学报,2011a,23(5):781~788.
5 潘洪彬,王静,黄英,等.饲粮能量水平对乌金猪脂肪组织脂类分解代谢相关基因表达的影响[J].动物营养学报,2011b,23(11):1946~1952.
6 Assaf S,Hazard D,Pitel F,et al. Cloning of cDNA encoding the nuclear form of chicken sterol response element binding protein-2 (srebp-2),chromosomal localization,and tissue expression of chicken SREBP-1 and -2 genes[J].Poultry Science,2003,1(82):54~61.
7 Brown M S,Goldstein J L. A proteolytic pathway that controls the cholesterol content of membranes,cells,and blood[J].Proceedings of the National Academy of Sciences of the United States of America,1999,96(20):11041~11048.
8 Cases S,Smith S J,Zheng Y W,et al. Identification of a gene encoding an acyl CoA: Diacylglycerol acyltransferase,a key enzyme in triacylglycerol synthesis[J].Proceedings of the National Academy of Sciences of the United States of America,1998,95(22):13018~13023.
9 Liu C Y,Grant A L,Kim K H,et al. Porcine somatotropin decreases acetyl-CoA carboxylase gene expression in porcine adipose tissue[J].Domestic Animal Endocrinology,1994,11(1):125~132.
10 Semenkovich C F. Regulation of fatty acid synthase(FAS)[J].Progress in Lipid Research,1997,36(1):43~53.
11 Smith S J,Cases S,Jensen D R,et al. Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking DGAT[J].Nature Genrtics,2000,25:87~90.
12 Stoeckman A K,Towle H C. The role of SREBP-1c in nutritional regulation of lipogenic enzyme gene expression[J].The Journal of Biological Chemistry,2002,277: 27029~27035.
13 Sul H S,Wang D. Nutritional and hormonal regulation of enzymes in fat synthesis:Studies of fatty acid synthase and mitochondrial glycerol-3-phosphate acyltransferase gene transcription[J].Annual Review of Nutrition,1998,18:331~351.
14 Wang J,Zhao S M,Song X L,et al. Low protein diet up-regulate intramuscular lipogenic gene expression and down-regulate lipolytic gene expression in growth-finishing pigs[J].Livestock Science,2012,148:119~128.
15 Yu Y H,Ginsberg H N. The role of acyl-CoA: Diacylglycerol acyltransferase (DGAT) in energy metabolism[J].Annals of Medicine,2004,36(4):252~261.
16 Zhang X,Zhao S M,Ge C R,et al. Effects of dietary digestible energy levels on meat quality in Wujin pig[J].Chinese Journal of Animal Nutrition,2008a,20(4):338~377.
17 Zhang X,Zhao S M,Ge C R,et al. Effects of dietary energy levels on growth performance and carcass composition of Wujin pigs[J]. Chinese Journal of Animal Nutrition,2008b,20(5):450~489.
18 Zhao S M,Wang J,Song X L,et al. Impact of dietary protein on lipid metabolism-related gene expression in porcine adipose tissue[J].Nutrition & Metabolism,2010,7(6):6~18.
19 Zhao S,Ma H,Zou S,et al. Hepatic lipogenesis in broiler chickens with different fat deposition during embryonic development[J]. Journal of Veterinary Medicine,2007,54(1):1~6.

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